A simple method to obtain endophytic microorganisms from field-collected roots

We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and...

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Autores principales:	Silvani, V.A., Fracchia, S., Fernández, L., Pérgola, M., Godeas, A.
Formato:	JOUR
Materias:	AMF Bacteria DSE Field roots In vitro culture Root endophytes DNA sequences Fungi Plant cell culture agar arbuscular mycorrhiza bacterium endophyte fungus methodology quantitative analysis rhizosphere root root vegetable Arbuscular Bacteria (microorganisms) Daucus carota
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00380717_v40_n5_p1259_Silvani
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00380717_v40_n5_p1259_Silvani
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spelling	todo:paper_00380717_v40_n5_p1259_Silvani2023-10-03T14:48:21Z A simple method to obtain endophytic microorganisms from field-collected roots Silvani, V.A. Fracchia, S. Fernández, L. Pérgola, M. Godeas, A. AMF Bacteria DSE Field roots In vitro culture Root endophytes Bacteria DNA sequences Fungi Field roots In vitro culture Root endophytes Plant cell culture agar arbuscular mycorrhiza bacterium endophyte fungus methodology quantitative analysis rhizosphere root root vegetable Arbuscular Bacteria (microorganisms) Daucus carota Fungi We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and those with bacteria and dark septate endophytic (DSE) fungi were plated onto tryptic soy agar (TSA) and malt extract agar (MEA), respectively. When arbuscular mycorrhizal fungi (AMF) grew from root segments, they were inoculated into Ri T-DNA-transformed carrot root cultures. Eight different species of bacteria and five dark septate endophytes were isolated. Thirty AMF isolates were identified and continuous pure cultures established. This easy and inexpensive approach allowed us to culture various endophytic microorganisms in an in vitro system saving time and space. © 2007 Elsevier Ltd. All rights reserved. Fil:Silvani, V.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fracchia, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernández, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pérgola, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Godeas, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00380717_v40_n5_p1259_Silvani
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	AMF Bacteria DSE Field roots In vitro culture Root endophytes Bacteria DNA sequences Fungi Field roots In vitro culture Root endophytes Plant cell culture agar arbuscular mycorrhiza bacterium endophyte fungus methodology quantitative analysis rhizosphere root root vegetable Arbuscular Bacteria (microorganisms) Daucus carota Fungi
spellingShingle	AMF Bacteria DSE Field roots In vitro culture Root endophytes Bacteria DNA sequences Fungi Field roots In vitro culture Root endophytes Plant cell culture agar arbuscular mycorrhiza bacterium endophyte fungus methodology quantitative analysis rhizosphere root root vegetable Arbuscular Bacteria (microorganisms) Daucus carota Fungi Silvani, V.A. Fracchia, S. Fernández, L. Pérgola, M. Godeas, A. A simple method to obtain endophytic microorganisms from field-collected roots
topic_facet	AMF Bacteria DSE Field roots In vitro culture Root endophytes Bacteria DNA sequences Fungi Field roots In vitro culture Root endophytes Plant cell culture agar arbuscular mycorrhiza bacterium endophyte fungus methodology quantitative analysis rhizosphere root root vegetable Arbuscular Bacteria (microorganisms) Daucus carota Fungi
description	We describe a simple technique for obtaining endophytic microorganisms from field-collected roots. Roots of plant species were surfaced-sterilized, cut into pieces, and then each root piece was transferred to drops of Gel-Gro medium. The number of segments with bacteria and fungi was quantified and those with bacteria and dark septate endophytic (DSE) fungi were plated onto tryptic soy agar (TSA) and malt extract agar (MEA), respectively. When arbuscular mycorrhizal fungi (AMF) grew from root segments, they were inoculated into Ri T-DNA-transformed carrot root cultures. Eight different species of bacteria and five dark septate endophytes were isolated. Thirty AMF isolates were identified and continuous pure cultures established. This easy and inexpensive approach allowed us to culture various endophytic microorganisms in an in vitro system saving time and space. © 2007 Elsevier Ltd. All rights reserved.
format	JOUR
author	Silvani, V.A. Fracchia, S. Fernández, L. Pérgola, M. Godeas, A.
author_facet	Silvani, V.A. Fracchia, S. Fernández, L. Pérgola, M. Godeas, A.
author_sort	Silvani, V.A.
title	A simple method to obtain endophytic microorganisms from field-collected roots
title_short	A simple method to obtain endophytic microorganisms from field-collected roots
title_full	A simple method to obtain endophytic microorganisms from field-collected roots
title_fullStr	A simple method to obtain endophytic microorganisms from field-collected roots
title_full_unstemmed	A simple method to obtain endophytic microorganisms from field-collected roots
title_sort	simple method to obtain endophytic microorganisms from field-collected roots
url	http://hdl.handle.net/20.500.12110/paper_00380717_v40_n5_p1259_Silvani
work_keys_str_mv	AT silvaniva asimplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT fracchias asimplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT fernandezl asimplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT pergolam asimplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT godeasa asimplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT silvaniva simplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT fracchias simplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT fernandezl simplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT pergolam simplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots AT godeasa simplemethodtoobtainendophyticmicroorganismsfromfieldcollectedroots
_version_	1807314949227151360

A simple method to obtain endophytic microorganisms from field-collected roots

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