O-glycosylated cell wall proteins are essential in root hair growth
Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early...
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todo:paper_00368075_v332_n6036_p1401_Velasquez2023-10-03T14:47:52Z O-glycosylated cell wall proteins are essential in root hair growth Velasquez, S.M. Ricardi, M.M. Dorosz, J.G. Fernandez, P.V. Nadra, A.D. Pol-Fachin, L. Egelund, J. Gille, S. Harholt, J. Ciancia, M. Verli, H. Pauly, M. Bacic, A. Olsen, C.E. Ulvskov, P. Petersen, B.L. Somerville, C. Iusem, N.D. Estevez, J.M. arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana. Fil:Velasquez, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ricardi, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Nadra, A.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Iusem, N.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00368075_v332_n6036_p1401_Velasquez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana |
spellingShingle |
arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana Velasquez, S.M. Ricardi, M.M. Dorosz, J.G. Fernandez, P.V. Nadra, A.D. Pol-Fachin, L. Egelund, J. Gille, S. Harholt, J. Ciancia, M. Verli, H. Pauly, M. Bacic, A. Olsen, C.E. Ulvskov, P. Petersen, B.L. Somerville, C. Iusem, N.D. Estevez, J.M. O-glycosylated cell wall proteins are essential in root hair growth |
topic_facet |
arabinogalactan arabinosyltransferase cell membrane protein extensin glycan glycoprotein green fluorescent protein hydroxyproline rich glycoprotein procollagen proline 2 oxoglutarate 4 dioxygenase proline rich protein transferase unclassified drug adsorption dicotyledon enzyme activity hair inhibition polysaccharide protein Arabidopsis arabinosylation article cell growth cell wall chemical analysis chemical reaction glycosylation hydroxylation nonhuman priority journal protein expression protein function root hair Arabidopsis Arabidopsis Proteins Arabinose Carbohydrate Conformation Cell Wall Gene Expression Regulation, Plant Genes, Plant Glycoproteins Glycosylation Glycosyltransferases Hydroxylation Hydroxyproline Models, Biological Mutation Pentosyltransferases Phenotype Plant Proteins Plant Roots Polysaccharides Procollagen-Proline Dioxygenase Proline Protein Conformation Protein Processing, Post-Translational Protein Structure, Secondary Arabidopsis thaliana |
description |
Root hairs are single cells that develop by tip growth and are specialized in the absorption of nutrients. Their cell walls are composed of polysaccharides and hydroxyproline-rich glycoproteins (HRGPs) that include extensins (EXTs) and arabinogalactan-proteins (AGPs). Proline hydroxylation, an early posttranslational modification of HRGPs that is catalyzed by prolyl 4-hydroxylases (P4Hs), defines the subsequent O-glycosylation sites in EXTs (which are mainly arabinosylated) and AGPs (which are mainly arabinogalactosylated). We explored the biological function of P4Hs, arabinosyltransferases, and EXTs in root hair cell growth. Biochemical inhibition or genetic disruption resulted in the blockage of polarized growth in root hairs and reduced arabinosylation of EXTs. Our results demonstrate that correct O-glycosylation on EXTs is essential for cell-wall self-assembly and, hence, root hair elongation in Arabidopsis thaliana. |
format |
JOUR |
author |
Velasquez, S.M. Ricardi, M.M. Dorosz, J.G. Fernandez, P.V. Nadra, A.D. Pol-Fachin, L. Egelund, J. Gille, S. Harholt, J. Ciancia, M. Verli, H. Pauly, M. Bacic, A. Olsen, C.E. Ulvskov, P. Petersen, B.L. Somerville, C. Iusem, N.D. Estevez, J.M. |
author_facet |
Velasquez, S.M. Ricardi, M.M. Dorosz, J.G. Fernandez, P.V. Nadra, A.D. Pol-Fachin, L. Egelund, J. Gille, S. Harholt, J. Ciancia, M. Verli, H. Pauly, M. Bacic, A. Olsen, C.E. Ulvskov, P. Petersen, B.L. Somerville, C. Iusem, N.D. Estevez, J.M. |
author_sort |
Velasquez, S.M. |
title |
O-glycosylated cell wall proteins are essential in root hair growth |
title_short |
O-glycosylated cell wall proteins are essential in root hair growth |
title_full |
O-glycosylated cell wall proteins are essential in root hair growth |
title_fullStr |
O-glycosylated cell wall proteins are essential in root hair growth |
title_full_unstemmed |
O-glycosylated cell wall proteins are essential in root hair growth |
title_sort |
o-glycosylated cell wall proteins are essential in root hair growth |
url |
http://hdl.handle.net/20.500.12110/paper_00368075_v332_n6036_p1401_Velasquez |
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