Differentiation of human embryonic stem cells into cardiomyocytes

Background: The role of stem cells in the treatment of several conditions, especially heart diseases, is under permanent investigation. Human embryonic stem cells have been successfully differentiated in vitro into cardiomyocytes. Methods of cell culture and cardiomyocyte differentiation are well es...

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Autores principales: Scassa, M.E., Fernández Espinosa, D., Questa, M., Videla Richardson, G., Losino, N., Luzzani, C., Guberman, A.S., Grancelli, H.O., Sevlever, G., Miriuka, S.G.
Formato: JOUR
Materias:
Cardiomyocyte
Embryonic stem cells
Myogenesis
structural protein
transcription factor
article
cell culture
cell differentiation
cell growth
embryonic stem cell
heart disease
heart muscle cell
human
human cell
immunohistochemistry
in vitro study
pluripotent stem cell
protein expression
protein structure
reverse transcription polymerase chain reaction
signal transduction
tissue regeneration
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00347000_v77_n6_p458_Scassa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00347000_v77_n6_p458_Scassa
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spelling todo:paper_00347000_v77_n6_p458_Scassa2023-10-03T14:46:16Z Differentiation of human embryonic stem cells into cardiomyocytes Scassa, M.E. Fernández Espinosa, D. Questa, M. Videla Richardson, G. Losino, N. Luzzani, C. Guberman, A.S. Grancelli, H.O. Sevlever, G. Miriuka, S.G. Cardiomyocyte Embryonic stem cells Myogenesis structural protein transcription factor article cell culture cell differentiation cell growth embryonic stem cell heart disease heart muscle cell human human cell immunohistochemistry in vitro study pluripotent stem cell protein expression protein structure reverse transcription polymerase chain reaction signal transduction tissue regeneration Background: The role of stem cells in the treatment of several conditions, especially heart diseases, is under permanent investigation. Human embryonic stem cells have been successfully differentiated in vitro into cardiomyocytes. Methods of cell culture and cardiomyocyte differentiation are well established; signals regulating cardiogenesis have been identified and the cardiomyocytes generated have been used in models of myocardial regeneration. However, several questions still remain and are currently under active investigation. Objective: To develop a culture system that is suitable for the induction of embryonic stem cells to cardiomyocyte differentiation. Material and Methods: Four human embryonic stem cell lines were used. The cells were cultured and differentiation was induced using methods previously described. The presence of cells in an undifferentiated state and cardiomyocyte differentiation was detected by immunohistochemical studies (fluorescent staining) and RT-PCR. Results: The methodology used allowed stem cells growth in the culture, and maintained them in an undifferentiated state. Cardiomyocyte differentiation was achieved in the four cell lines used, yet with uneven efficacy. This was confirmed by the expression of myocardial transcription factors and heart structural proteins. Conclusions: Our system allowed human embryonic stem cell growth and differentiation in the culture. These preliminary results encourage us to continue developing our methods with induced pluripotent stem cells. Fil:Scassa, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Questa, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Losino, N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Luzzani, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00347000_v77_n6_p458_Scassa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cardiomyocyte
Embryonic stem cells
Myogenesis
structural protein
transcription factor
article
cell culture
cell differentiation
cell growth
embryonic stem cell
heart disease
heart muscle cell
human
human cell
immunohistochemistry
in vitro study
pluripotent stem cell
protein expression
protein structure
reverse transcription polymerase chain reaction
signal transduction
tissue regeneration
spellingShingle Cardiomyocyte
Embryonic stem cells
Myogenesis
structural protein
transcription factor
article
cell culture
cell differentiation
cell growth
embryonic stem cell
heart disease
heart muscle cell
human
human cell
immunohistochemistry
in vitro study
pluripotent stem cell
protein expression
protein structure
reverse transcription polymerase chain reaction
signal transduction
tissue regeneration
Scassa, M.E.
Fernández Espinosa, D.
Questa, M.
Videla Richardson, G.
Losino, N.
Luzzani, C.
Guberman, A.S.
Grancelli, H.O.
Sevlever, G.
Miriuka, S.G.
Differentiation of human embryonic stem cells into cardiomyocytes
topic_facet Cardiomyocyte
Embryonic stem cells
Myogenesis
structural protein
transcription factor
article
cell culture
cell differentiation
cell growth
embryonic stem cell
heart disease
heart muscle cell
human
human cell
immunohistochemistry
in vitro study
pluripotent stem cell
protein expression
protein structure
reverse transcription polymerase chain reaction
signal transduction
tissue regeneration
description Background: The role of stem cells in the treatment of several conditions, especially heart diseases, is under permanent investigation. Human embryonic stem cells have been successfully differentiated in vitro into cardiomyocytes. Methods of cell culture and cardiomyocyte differentiation are well established; signals regulating cardiogenesis have been identified and the cardiomyocytes generated have been used in models of myocardial regeneration. However, several questions still remain and are currently under active investigation. Objective: To develop a culture system that is suitable for the induction of embryonic stem cells to cardiomyocyte differentiation. Material and Methods: Four human embryonic stem cell lines were used. The cells were cultured and differentiation was induced using methods previously described. The presence of cells in an undifferentiated state and cardiomyocyte differentiation was detected by immunohistochemical studies (fluorescent staining) and RT-PCR. Results: The methodology used allowed stem cells growth in the culture, and maintained them in an undifferentiated state. Cardiomyocyte differentiation was achieved in the four cell lines used, yet with uneven efficacy. This was confirmed by the expression of myocardial transcription factors and heart structural proteins. Conclusions: Our system allowed human embryonic stem cell growth and differentiation in the culture. These preliminary results encourage us to continue developing our methods with induced pluripotent stem cells.
format JOUR
author Scassa, M.E.
Fernández Espinosa, D.
Questa, M.
Videla Richardson, G.
Losino, N.
Luzzani, C.
Guberman, A.S.
Grancelli, H.O.
Sevlever, G.
Miriuka, S.G.
author_facet Scassa, M.E.
Fernández Espinosa, D.
Questa, M.
Videla Richardson, G.
Losino, N.
Luzzani, C.
Guberman, A.S.
Grancelli, H.O.
Sevlever, G.
Miriuka, S.G.
author_sort Scassa, M.E.
title Differentiation of human embryonic stem cells into cardiomyocytes
title_short Differentiation of human embryonic stem cells into cardiomyocytes
title_full Differentiation of human embryonic stem cells into cardiomyocytes
title_fullStr Differentiation of human embryonic stem cells into cardiomyocytes
title_full_unstemmed Differentiation of human embryonic stem cells into cardiomyocytes
title_sort differentiation of human embryonic stem cells into cardiomyocytes
url http://hdl.handle.net/20.500.12110/paper_00347000_v77_n6_p458_Scassa
work_keys_str_mv AT scassame differentiationofhumanembryonicstemcellsintocardiomyocytes
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