Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)

Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed ba...

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Detalles Bibliográficos
Autores principales: Pavan, M.E., Robles, C., Cairó, F.M., Marcellino, R., Pettinari, M.J.
Formato: JOUR
Materias:
Caseous lymphadenitis
Corynebacterium pseudotuberculosis
RpoB PCR-RFLP
endonuclease
protein MseI
protein StuI
unclassified drug
analytic method
Arcanobacterium pyogenes
article
bacterial gene
bacterium identification
bacterium isolation
computer analysis
controlled study
Corynebacterium ulcerans
molecular typing
nonhuman
nucleotide sequence
polymerase chain reaction
reliability
restriction fragment length polymorphism
RNA polymerase beta subunit gene
rpoB gene
sequence analysis
sheep
species differentiation
unindexed sequence
wild type
Animals
Corynebacterium Infections
DNA-Directed RNA Polymerases
Polymorphism, Restriction Fragment Length
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis
Sheep
Sheep Diseases
Animalia
Capra
Corynebacterium
Ovis
Ovis aries
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00345288_v92_n2_p202_Pavan
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase β-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR-RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies. © 2011 Elsevier Ltd.

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