Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)

Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed ba...

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Autores principales:	Pavan, M.E., Robles, C., Cairó, F.M., Marcellino, R., Pettinari, M.J.
Formato:	JOUR
Materias:	Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Capra Corynebacterium Ovis Ovis aries
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00345288_v92_n2_p202_Pavan
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00345288_v92_n2_p202_Pavan
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spelling	todo:paper_00345288_v92_n2_p202_Pavan2023-10-03T14:45:56Z Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB) Pavan, M.E. Robles, C. Cairó, F.M. Marcellino, R. Pettinari, M.J. Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium pseudotuberculosis Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections Corynebacterium pseudotuberculosis DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Arcanobacterium pyogenes Capra Corynebacterium Corynebacterium pseudotuberculosis Ovis Ovis aries Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase β-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR-RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies. © 2011 Elsevier Ltd. Fil:Pettinari, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00345288_v92_n2_p202_Pavan
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium pseudotuberculosis Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections Corynebacterium pseudotuberculosis DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Arcanobacterium pyogenes Capra Corynebacterium Corynebacterium pseudotuberculosis Ovis Ovis aries
spellingShingle	Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium pseudotuberculosis Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections Corynebacterium pseudotuberculosis DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Arcanobacterium pyogenes Capra Corynebacterium Corynebacterium pseudotuberculosis Ovis Ovis aries Pavan, M.E. Robles, C. Cairó, F.M. Marcellino, R. Pettinari, M.J. Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
topic_facet	Caseous lymphadenitis Corynebacterium pseudotuberculosis RpoB PCR-RFLP endonuclease protein MseI protein StuI unclassified drug analytic method Arcanobacterium pyogenes article bacterial gene bacterium identification bacterium isolation computer analysis controlled study Corynebacterium pseudotuberculosis Corynebacterium ulcerans molecular typing nonhuman nucleotide sequence polymerase chain reaction reliability restriction fragment length polymorphism RNA polymerase beta subunit gene rpoB gene sequence analysis sheep species differentiation unindexed sequence wild type Animals Corynebacterium Infections Corynebacterium pseudotuberculosis DNA-Directed RNA Polymerases Polymorphism, Restriction Fragment Length Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Sheep Sheep Diseases Animalia Arcanobacterium pyogenes Capra Corynebacterium Corynebacterium pseudotuberculosis Ovis Ovis aries
description	Caseous lymphadenitis, caused by Corynebacterium pseudotuberculosis, has a high prevalence in many regions of the world, including Argentina and Brazil. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for the identification of this microorganism was designed based on the hypervariable region of the polymorphic RNA polymerase β-subunit gene (rpoB). All available Corynebacterium rpoB sequences were analyzed by computer-assisted restriction analysis. The rpoB PCR-RFLP pattern predicted by using endonucleases MseI and StuI clearly differentiated C. pseudotuberculosis from sixty-one other Corynebacterium species. This method was successfully applied to identify twelve wild C. pseudotuberculosis ovine isolates and one caprine isolate. It was also used to differentiate C. pseudotuberculosis from Arcanobacterium pyogenes, an ovine pathogen with similar clinical characteristics. These results indicate that this new molecular method can be used for the reliable identification of the pathogen, essential for the timely detection of infected animals and for epidemiological studies. © 2011 Elsevier Ltd.
format	JOUR
author	Pavan, M.E. Robles, C. Cairó, F.M. Marcellino, R. Pettinari, M.J.
author_facet	Pavan, M.E. Robles, C. Cairó, F.M. Marcellino, R. Pettinari, M.J.
author_sort	Pavan, M.E.
title	Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_short	Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_full	Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_fullStr	Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_full_unstemmed	Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)
title_sort	identification of corynebacterium pseudotuberculosis from sheep by pcr-restriction analysis using the rna polymerase β-subunit gene (rpob)
url	http://hdl.handle.net/20.500.12110/paper_00345288_v92_n2_p202_Pavan
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Identification of Corynebacterium pseudotuberculosis from sheep by PCR-restriction analysis using the RNA polymerase β-subunit gene (rpoB)

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