Screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase
In order to examine inhibitory effects of hexachlorobenzene metabolites on the hepatic porphyrinogen carboxylyase activity, rat liver cytosol was incubated with uroporphyrinogen III and chlorinated phenols, thiophenols, thioanisoles and benzenes. Then, the occurrence of hepta-, hexa-, penta- and tet...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00345164_v51_n3_p325_Billi |
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todo:paper_00345164_v51_n3_p325_Billi2023-10-03T14:45:55Z Screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase Billi, S.C. Koss, G. San Martin de Viale, L.C. hexachlorobenzene pentachlorophenol tetrachlorohydroquinone uroporphyrinogen decarboxylase animal experiment enzyme inhibition liver metabolite nonhuman pentachlorobenzenethiol priority journal tetrachlorobenzene Animal Carboxy-Lyases Chlorobenzenes Chlorophenols Cytosol Female Hexachlorobenzene Liver Rats Support, Non-U.S. Gov't Uroporphyrinogens In order to examine inhibitory effects of hexachlorobenzene metabolites on the hepatic porphyrinogen carboxylyase activity, rat liver cytosol was incubated with uroporphyrinogen III and chlorinated phenols, thiophenols, thioanisoles and benzenes. Then, the occurrence of hepta-, hexa-, penta- and tetracarboxyporphyrinogen = coproporphyrinogen (measured as porphyrins) was determined. - Inhibitory effects were exerted by tetrachlorohydroquinone, pentachlorophenol, pentachlorothiophenol, 1,2,3,5- and 1,2,4,5- tetrachlorobenzene. Other compounds including hexachlorobenzene which was tested for comparative reasons did not impair uroporphyrinogen decarboxylation. - In the presence of tetrachlorohydroquinone, uroporphyrinogen merely was decarboxylated to hepta- and hexacarboxyporphyrinogen. Under the influence of the other 4 compounds with inhibitory effects, pentacarboxyporphyrinogen and coproporphyrinogen were formed additionally. - Coproporphyrinogen formation was inhibited completely by tetrachlorohydroquinone, while pentachlorophenol diminished its formation by about 50% and pentachlorothiophenol, 1,2,3,5- and 1,2,4,5-tetrachlorobenzene by less than 10%. Fil:Billi, S.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:San Martin de Viale, L.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00345164_v51_n3_p325_Billi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
hexachlorobenzene pentachlorophenol tetrachlorohydroquinone uroporphyrinogen decarboxylase animal experiment enzyme inhibition liver metabolite nonhuman pentachlorobenzenethiol priority journal tetrachlorobenzene Animal Carboxy-Lyases Chlorobenzenes Chlorophenols Cytosol Female Hexachlorobenzene Liver Rats Support, Non-U.S. Gov't Uroporphyrinogens |
| spellingShingle |
hexachlorobenzene pentachlorophenol tetrachlorohydroquinone uroporphyrinogen decarboxylase animal experiment enzyme inhibition liver metabolite nonhuman pentachlorobenzenethiol priority journal tetrachlorobenzene Animal Carboxy-Lyases Chlorobenzenes Chlorophenols Cytosol Female Hexachlorobenzene Liver Rats Support, Non-U.S. Gov't Uroporphyrinogens Billi, S.C. Koss, G. San Martin de Viale, L.C. Screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase |
| topic_facet |
hexachlorobenzene pentachlorophenol tetrachlorohydroquinone uroporphyrinogen decarboxylase animal experiment enzyme inhibition liver metabolite nonhuman pentachlorobenzenethiol priority journal tetrachlorobenzene Animal Carboxy-Lyases Chlorobenzenes Chlorophenols Cytosol Female Hexachlorobenzene Liver Rats Support, Non-U.S. Gov't Uroporphyrinogens |
| description |
In order to examine inhibitory effects of hexachlorobenzene metabolites on the hepatic porphyrinogen carboxylyase activity, rat liver cytosol was incubated with uroporphyrinogen III and chlorinated phenols, thiophenols, thioanisoles and benzenes. Then, the occurrence of hepta-, hexa-, penta- and tetracarboxyporphyrinogen = coproporphyrinogen (measured as porphyrins) was determined. - Inhibitory effects were exerted by tetrachlorohydroquinone, pentachlorophenol, pentachlorothiophenol, 1,2,3,5- and 1,2,4,5- tetrachlorobenzene. Other compounds including hexachlorobenzene which was tested for comparative reasons did not impair uroporphyrinogen decarboxylation. - In the presence of tetrachlorohydroquinone, uroporphyrinogen merely was decarboxylated to hepta- and hexacarboxyporphyrinogen. Under the influence of the other 4 compounds with inhibitory effects, pentacarboxyporphyrinogen and coproporphyrinogen were formed additionally. - Coproporphyrinogen formation was inhibited completely by tetrachlorohydroquinone, while pentachlorophenol diminished its formation by about 50% and pentachlorothiophenol, 1,2,3,5- and 1,2,4,5-tetrachlorobenzene by less than 10%. |
| format |
JOUR |
| author |
Billi, S.C. Koss, G. San Martin de Viale, L.C. |
| author_facet |
Billi, S.C. Koss, G. San Martin de Viale, L.C. |
| author_sort |
Billi, S.C. |
| title |
Screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase |
| title_short |
Screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase |
| title_full |
Screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase |
| title_fullStr |
Screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase |
| title_full_unstemmed |
Screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase |
| title_sort |
screening for the ability of hexachlorobenzene metabolites to decrease rat liver porphyrinogen carboxylyase |
| url |
http://hdl.handle.net/20.500.12110/paper_00345164_v51_n3_p325_Billi |
| work_keys_str_mv |
AT billisc screeningfortheabilityofhexachlorobenzenemetabolitestodecreaseratliverporphyrinogencarboxylyase AT kossg screeningfortheabilityofhexachlorobenzenemetabolitestodecreaseratliverporphyrinogencarboxylyase AT sanmartindevialelc screeningfortheabilityofhexachlorobenzenemetabolitestodecreaseratliverporphyrinogencarboxylyase |
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