On-line detection of low naphthalene concentrations with a bioluminescent sensor

On-line tests with Pseudomonas fluorescens HK44 were carried out using a biosensor system with a flow-through cell and sample injections to detect low concentrations of naphthalene. Kinetic experiments permitted a 19 min response time and definition of a 50 min induction period for bioluminescence a...

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Autores principales:	Valdman, E., Valdman, B., Battaglini, F., Leite, S.G.F.
Formato:	JOUR
Materias:	Bioluminescence Naphthalene On-line detection Ps. fluorescens HK44 Pseudomonas Pseudomonas fluorescens
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00329592_v39_n10_p1217_Valdman
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00329592_v39_n10_p1217_Valdman2023-10-03T14:45:29Z On-line detection of low naphthalene concentrations with a bioluminescent sensor Valdman, E. Valdman, B. Battaglini, F. Leite, S.G.F. Bioluminescence Naphthalene On-line detection Ps. fluorescens HK44 Pseudomonas Pseudomonas fluorescens On-line tests with Pseudomonas fluorescens HK44 were carried out using a biosensor system with a flow-through cell and sample injections to detect low concentrations of naphthalene. Kinetic experiments permitted a 19 min response time and definition of a 50 min induction period for bioluminescence assays. A mathematical model showed that 0.2 g/l is the best cell concentration to be used in the detection of low naphthalene concentrations, with the highest acceleration rate of bioluminescence sensitivity increase (0.46 nA l/(g min 2)). Calibration curves with different concentrations of naphthalene showed a linear correlation up to 0.4 mg/l (3 μM) corresponding to 211 nA output signal. The lower limit of naphthalene detection by strain HK44 was in the region of 0.02 mg/l (0.16 μM), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA. © 2003 Elsevier Ltd. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00329592_v39_n10_p1217_Valdman
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Bioluminescence Naphthalene On-line detection Ps. fluorescens HK44 Pseudomonas Pseudomonas fluorescens
spellingShingle	Bioluminescence Naphthalene On-line detection Ps. fluorescens HK44 Pseudomonas Pseudomonas fluorescens Valdman, E. Valdman, B. Battaglini, F. Leite, S.G.F. On-line detection of low naphthalene concentrations with a bioluminescent sensor
topic_facet	Bioluminescence Naphthalene On-line detection Ps. fluorescens HK44 Pseudomonas Pseudomonas fluorescens
description	On-line tests with Pseudomonas fluorescens HK44 were carried out using a biosensor system with a flow-through cell and sample injections to detect low concentrations of naphthalene. Kinetic experiments permitted a 19 min response time and definition of a 50 min induction period for bioluminescence assays. A mathematical model showed that 0.2 g/l is the best cell concentration to be used in the detection of low naphthalene concentrations, with the highest acceleration rate of bioluminescence sensitivity increase (0.46 nA l/(g min 2)). Calibration curves with different concentrations of naphthalene showed a linear correlation up to 0.4 mg/l (3 μM) corresponding to 211 nA output signal. The lower limit of naphthalene detection by strain HK44 was in the region of 0.02 mg/l (0.16 μM), below the naphthalene health advisory limit for drinking water consumed over a lifetime suggested by EPA. © 2003 Elsevier Ltd. All rights reserved.
format	JOUR
author	Valdman, E. Valdman, B. Battaglini, F. Leite, S.G.F.
author_facet	Valdman, E. Valdman, B. Battaglini, F. Leite, S.G.F.
author_sort	Valdman, E.
title	On-line detection of low naphthalene concentrations with a bioluminescent sensor
title_short	On-line detection of low naphthalene concentrations with a bioluminescent sensor
title_full	On-line detection of low naphthalene concentrations with a bioluminescent sensor
title_fullStr	On-line detection of low naphthalene concentrations with a bioluminescent sensor
title_full_unstemmed	On-line detection of low naphthalene concentrations with a bioluminescent sensor
title_sort	on-line detection of low naphthalene concentrations with a bioluminescent sensor
url	http://hdl.handle.net/20.500.12110/paper_00329592_v39_n10_p1217_Valdman
work_keys_str_mv	AT valdmane onlinedetectionoflownaphthaleneconcentrationswithabioluminescentsensor AT valdmanb onlinedetectionoflownaphthaleneconcentrationswithabioluminescentsensor AT battaglinif onlinedetectionoflownaphthaleneconcentrationswithabioluminescentsensor AT leitesgf onlinedetectionoflownaphthaleneconcentrationswithabioluminescentsensor
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On-line detection of low naphthalene concentrations with a bioluminescent sensor

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