The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs

Mostrar todas las versiones(2)

Calcium-dependent protein kinases (CDPKs) are key components of calcium regulated signaling cascades in plants. In this work, isoform StCDPK3 from Solanum tuberosum was studied and fully described. StCDPK3 encodes a 63 kDa protein with an N-terminal variable domain (NTV), rich in prolines and glutam...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Grandellis, C., Giammaria, V., Bialer, M., Santin, F., Lin, T., Hannapel, D.J., Ulloa, R.M.
Formato:	JOUR
Materias:	Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Stress, Physiological Agrobacterium tumefaciens
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00320935_v236_n6_p1831_Grandellis
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00320935_v236_n6_p1831_Grandellis
record_format	dspace
spelling	todo:paper_00320935_v236_n6_p1831_Grandellis2023-10-03T14:45:04Z The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs Grandellis, C. Giammaria, V. Bialer, M. Santin, F. Lin, T. Hannapel, D.J. Ulloa, R.M. Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Solanum tuberosum Stress, Physiological Agrobacterium tumefaciens Solanum tuberosum Calcium-dependent protein kinases (CDPKs) are key components of calcium regulated signaling cascades in plants. In this work, isoform StCDPK3 from Solanum tuberosum was studied and fully described. StCDPK3 encodes a 63 kDa protein with an N-terminal variable domain (NTV), rich in prolines and glutamines, which presents myristoylation and palmitoylation consensus sites and a PEST sequence indicative of rapid protein degradation. StCDPK3 gene (circa 11 kb) is localized in chromosome 3, shares the eight exons and seven introns structure with other isoforms from subgroup IIa and contains an additional intron in the 5′UTR region. StCDPK3 expression is ubiquitous being transcripts more abundant in early elongating stolons (ES), leaves and roots, however isoform specific antibodies only detected the protein in leaf particulate extracts. The recombinant 6xHis-StCDPK3 is an active kinase that differs in its kinetic parameters and calcium requirements from StCDPK1 and 2 isoforms. In vitro, StCDPK3 undergoes autophosphorylation regardless of the addition of calcium. The StCDPK3 promoter region (circa 1,800 bp) was subcloned by genome walking and fused to GUS. Light and ABRE responsive elements were identified in the promoter region as well as elements associated to expression in roots. StCDPK3 expression was enhanced by ABA while GA decreased it. Potato transgenic lines harboring StCDPK3 promoter{proportion}GUS construct were generated by Agrobacterium tumefaciens mediated plant transformation. Promoter activity was detected in leaves, root tips and branching points, early ES, tuber eyes and developing sprouts indicating that StCDPK3 is expressed in actively growing organs. © 2012 Springer-Verlag. Fil:Grandellis, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Giammaria, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320935_v236_n6_p1831_Grandellis
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Solanum tuberosum Stress, Physiological Agrobacterium tumefaciens Solanum tuberosum
spellingShingle	Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Solanum tuberosum Stress, Physiological Agrobacterium tumefaciens Solanum tuberosum Grandellis, C. Giammaria, V. Bialer, M. Santin, F. Lin, T. Hannapel, D.J. Ulloa, R.M. The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs
topic_facet	Calcium dependent protein kinases Phytohormone responses Signaling Solanum tuberosum calcium protein kinase calcium-dependent protein kinase hybrid protein isoprotein plant RNA protein kinase vegetable protein amino acid sequence antibody specificity article DNA sequence enzymology gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence phosphorylation phylogeny physiological stress physiology plant leaf plant tuber potato promoter region sequence alignment signal transduction transgenic plant Amino Acid Sequence Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Molecular Sequence Data Organ Specificity Phosphorylation Phylogeny Plant Leaves Plant Proteins Plant Tubers Plants, Genetically Modified Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Fusion Proteins RNA, Plant Sequence Alignment Sequence Analysis, DNA Signal Transduction Solanum tuberosum Stress, Physiological Agrobacterium tumefaciens Solanum tuberosum
description	Calcium-dependent protein kinases (CDPKs) are key components of calcium regulated signaling cascades in plants. In this work, isoform StCDPK3 from Solanum tuberosum was studied and fully described. StCDPK3 encodes a 63 kDa protein with an N-terminal variable domain (NTV), rich in prolines and glutamines, which presents myristoylation and palmitoylation consensus sites and a PEST sequence indicative of rapid protein degradation. StCDPK3 gene (circa 11 kb) is localized in chromosome 3, shares the eight exons and seven introns structure with other isoforms from subgroup IIa and contains an additional intron in the 5′UTR region. StCDPK3 expression is ubiquitous being transcripts more abundant in early elongating stolons (ES), leaves and roots, however isoform specific antibodies only detected the protein in leaf particulate extracts. The recombinant 6xHis-StCDPK3 is an active kinase that differs in its kinetic parameters and calcium requirements from StCDPK1 and 2 isoforms. In vitro, StCDPK3 undergoes autophosphorylation regardless of the addition of calcium. The StCDPK3 promoter region (circa 1,800 bp) was subcloned by genome walking and fused to GUS. Light and ABRE responsive elements were identified in the promoter region as well as elements associated to expression in roots. StCDPK3 expression was enhanced by ABA while GA decreased it. Potato transgenic lines harboring StCDPK3 promoter{proportion}GUS construct were generated by Agrobacterium tumefaciens mediated plant transformation. Promoter activity was detected in leaves, root tips and branching points, early ES, tuber eyes and developing sprouts indicating that StCDPK3 is expressed in actively growing organs. © 2012 Springer-Verlag.
format	JOUR
author	Grandellis, C. Giammaria, V. Bialer, M. Santin, F. Lin, T. Hannapel, D.J. Ulloa, R.M.
author_facet	Grandellis, C. Giammaria, V. Bialer, M. Santin, F. Lin, T. Hannapel, D.J. Ulloa, R.M.
author_sort	Grandellis, C.
title	The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs
title_short	The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs
title_full	The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs
title_fullStr	The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs
title_full_unstemmed	The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs
title_sort	novel solanum tuberosum calcium dependent protein kinase, stcdpk3, is expressed in actively growing organs
url	http://hdl.handle.net/20.500.12110/paper_00320935_v236_n6_p1831_Grandellis
work_keys_str_mv	AT grandellisc thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT giammariav thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT bialerm thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT santinf thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT lint thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT hannapeldj thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT ulloarm thenovelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT grandellisc novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT giammariav novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT bialerm novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT santinf novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT lint novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT hannapeldj novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans AT ulloarm novelsolanumtuberosumcalciumdependentproteinkinasestcdpk3isexpressedinactivelygrowingorgans
_version_	1807316063592906752

The novel Solanum tuberosum calcium dependent protein kinase, StCDPK3, is expressed in actively growing organs

Ejemplares similares