Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress

Plasma membrane proton pumps (PM H+-ATPases) are involved in several physiological processes, such as growth and development, and abiotic stress responses. The major regulators of the PM H+-ATPases are proteins of the 14-3-3 family, which stimulate its activity. In addition, a novel interaction part...

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Autores principales:	García, M.N.M., País, S.M., Téllez-Iñón, M.T., Capiati, D.A.
Formato:	JOUR
Materias:	Plasma membrane H+-ATPase Proton pump interactor Solanum tuberosum L. StPPI1 Stress Tuber development proton pump proton transporting adenosine triphosphatase recombinant protein vegetable protein amino acid sequence antibody specificity article cell membrane chemistry enzymology gene expression profiling gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence pH physiological stress plant gene plant tuber potato sequence alignment Southern blotting tomato upregulation Amino Acid Sequence Blotting, Southern Cell Membrane Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Hydrogen-Ion Concentration Lycopersicon esculentum Molecular Sequence Data Organ Specificity Plant Proteins Plant Tubers Proton Pumps Proton-Translocating ATPases Recombinant Proteins Sequence Alignment Solanum tuberosum Stress, Physiological Up-Regulation Arabidopsis Arabidopsis thaliana Tuber (truffle)
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00320935_v233_n4_p661_Garcia
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00320935_v233_n4_p661_Garcia
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spelling	todo:paper_00320935_v233_n4_p661_Garcia2023-10-03T14:45:02Z Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress García, M.N.M. País, S.M. Téllez-Iñón, M.T. Capiati, D.A. Plasma membrane H+-ATPase Proton pump interactor Solanum tuberosum L. StPPI1 Stress Tuber development proton pump proton transporting adenosine triphosphatase recombinant protein vegetable protein amino acid sequence antibody specificity article cell membrane chemistry enzymology gene expression profiling gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence pH physiological stress plant gene plant tuber potato sequence alignment Southern blotting tomato upregulation Amino Acid Sequence Blotting, Southern Cell Membrane Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Hydrogen-Ion Concentration Lycopersicon esculentum Molecular Sequence Data Organ Specificity Plant Proteins Plant Tubers Proton Pumps Proton-Translocating ATPases Recombinant Proteins Sequence Alignment Solanum tuberosum Stress, Physiological Up-Regulation Arabidopsis Arabidopsis thaliana Lycopersicon esculentum Solanum tuberosum Tuber (truffle) Plasma membrane proton pumps (PM H+-ATPases) are involved in several physiological processes, such as growth and development, and abiotic stress responses. The major regulators of the PM H+-ATPases are proteins of the 14-3-3 family, which stimulate its activity. In addition, a novel interaction partner of the AHA1 PM H+-ATPase, named PPI1 (proton pump interactor, isoform 1), was identified in Arabidopsis thaliana. This protein stimulates the activity of the proton pump in vitro. In this work, we report the characterization of an A. thaliana PPI1 homolog in potato (Solanum tuberosum L.) named StPPI1. The full-length coding sequence of StPPI1 was obtained. The open reading frame (ORF) encodes a protein of 629 amino acids showing 50% identity with A. thaliana PPI1 protein. The StPPI1 ORF is divided into seven exons split by six introns. Southern blot analysis suggests that StPPI1 belongs to a family of related genes. Recombinant StPPI1 stimulates H+-ATPase activity in vitro. Basal levels of StPPI1 transcripts are observed in all tissues, however, StPPI1 expression is higher in proliferative regions (shoot apex and flower buds), flowers and leaves than in shoots and roots. StPPI1 mRNA levels significantly increase during tuber development. StPPI1 is induced by salt stress and cold. Drought and mechanical wounding slightly increase StPPI1 transcript levels. In addition, the expression of SlPPI1, the tomato homolog of StPPI1, was determined under adverse environmental conditions in tomato plants. SlPPI1 mRNA levels are increased by drought and cold, but are unaffected by salt stress. Mechanical wounding slightly increases SlPPI1 expression. © 2010 Springer-Verlag. Fil:País, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320935_v233_n4_p661_Garcia
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Plasma membrane H+-ATPase Proton pump interactor Solanum tuberosum L. StPPI1 Stress Tuber development proton pump proton transporting adenosine triphosphatase recombinant protein vegetable protein amino acid sequence antibody specificity article cell membrane chemistry enzymology gene expression profiling gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence pH physiological stress plant gene plant tuber potato sequence alignment Southern blotting tomato upregulation Amino Acid Sequence Blotting, Southern Cell Membrane Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Hydrogen-Ion Concentration Lycopersicon esculentum Molecular Sequence Data Organ Specificity Plant Proteins Plant Tubers Proton Pumps Proton-Translocating ATPases Recombinant Proteins Sequence Alignment Solanum tuberosum Stress, Physiological Up-Regulation Arabidopsis Arabidopsis thaliana Lycopersicon esculentum Solanum tuberosum Tuber (truffle)
spellingShingle	Plasma membrane H+-ATPase Proton pump interactor Solanum tuberosum L. StPPI1 Stress Tuber development proton pump proton transporting adenosine triphosphatase recombinant protein vegetable protein amino acid sequence antibody specificity article cell membrane chemistry enzymology gene expression profiling gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence pH physiological stress plant gene plant tuber potato sequence alignment Southern blotting tomato upregulation Amino Acid Sequence Blotting, Southern Cell Membrane Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Hydrogen-Ion Concentration Lycopersicon esculentum Molecular Sequence Data Organ Specificity Plant Proteins Plant Tubers Proton Pumps Proton-Translocating ATPases Recombinant Proteins Sequence Alignment Solanum tuberosum Stress, Physiological Up-Regulation Arabidopsis Arabidopsis thaliana Lycopersicon esculentum Solanum tuberosum Tuber (truffle) García, M.N.M. País, S.M. Téllez-Iñón, M.T. Capiati, D.A. Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress
topic_facet	Plasma membrane H+-ATPase Proton pump interactor Solanum tuberosum L. StPPI1 Stress Tuber development proton pump proton transporting adenosine triphosphatase recombinant protein vegetable protein amino acid sequence antibody specificity article cell membrane chemistry enzymology gene expression profiling gene expression regulation genetics growth, development and aging metabolism molecular genetics nucleotide sequence pH physiological stress plant gene plant tuber potato sequence alignment Southern blotting tomato upregulation Amino Acid Sequence Blotting, Southern Cell Membrane Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Hydrogen-Ion Concentration Lycopersicon esculentum Molecular Sequence Data Organ Specificity Plant Proteins Plant Tubers Proton Pumps Proton-Translocating ATPases Recombinant Proteins Sequence Alignment Solanum tuberosum Stress, Physiological Up-Regulation Arabidopsis Arabidopsis thaliana Lycopersicon esculentum Solanum tuberosum Tuber (truffle)
description	Plasma membrane proton pumps (PM H+-ATPases) are involved in several physiological processes, such as growth and development, and abiotic stress responses. The major regulators of the PM H+-ATPases are proteins of the 14-3-3 family, which stimulate its activity. In addition, a novel interaction partner of the AHA1 PM H+-ATPase, named PPI1 (proton pump interactor, isoform 1), was identified in Arabidopsis thaliana. This protein stimulates the activity of the proton pump in vitro. In this work, we report the characterization of an A. thaliana PPI1 homolog in potato (Solanum tuberosum L.) named StPPI1. The full-length coding sequence of StPPI1 was obtained. The open reading frame (ORF) encodes a protein of 629 amino acids showing 50% identity with A. thaliana PPI1 protein. The StPPI1 ORF is divided into seven exons split by six introns. Southern blot analysis suggests that StPPI1 belongs to a family of related genes. Recombinant StPPI1 stimulates H+-ATPase activity in vitro. Basal levels of StPPI1 transcripts are observed in all tissues, however, StPPI1 expression is higher in proliferative regions (shoot apex and flower buds), flowers and leaves than in shoots and roots. StPPI1 mRNA levels significantly increase during tuber development. StPPI1 is induced by salt stress and cold. Drought and mechanical wounding slightly increase StPPI1 transcript levels. In addition, the expression of SlPPI1, the tomato homolog of StPPI1, was determined under adverse environmental conditions in tomato plants. SlPPI1 mRNA levels are increased by drought and cold, but are unaffected by salt stress. Mechanical wounding slightly increases SlPPI1 expression. © 2010 Springer-Verlag.
format	JOUR
author	García, M.N.M. País, S.M. Téllez-Iñón, M.T. Capiati, D.A.
author_facet	García, M.N.M. País, S.M. Téllez-Iñón, M.T. Capiati, D.A.
author_sort	García, M.N.M.
title	Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress
title_short	Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress
title_full	Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress
title_fullStr	Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress
title_full_unstemmed	Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress
title_sort	characterization of stppi1, a proton pump interactor from solanum tuberosum l. that is up-regulated during tuber development and by abiotic stress
url	http://hdl.handle.net/20.500.12110/paper_00320935_v233_n4_p661_Garcia
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Characterization of StPPI1, a proton pump interactor from Solanum tuberosum L. that is up-regulated during tuber development and by abiotic stress

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