StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling
Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome lo...
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todo:paper_00320935_v233_n3_p593_Giammaria2023-10-03T14:45:02Z StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling Giammaria, V. Grandellis, C. Bachmann, S. Gargantini, P.R. Feingold, S.E. Bryan, G. Ulloa, R.M. Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Light Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Up-Regulation Solanum tuberosum Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome located in chromosome 7. StCDPK2 encodes an active CDPK of 515 aminoacids, with an apparent MW of 57 kDa, which presents myristoylation and palmitoylation consensus in its N-terminus. StCDPK2 is highly expressed in leaves and green sprouts; enhanced expression was detected under light treatment, which corresponds well with light responsive cis-acting elements found in its promoter sequence. Antibodies against the recombinant StCDPK2::6xHis protein detected this isoform in soluble and particulate fractions from leaves. StCDPK2 autophosphorylation and kinase activity are both calcium dependent reaching half maximal activation at 0.6 μM calcium. The active kinase is autophosphorylated on serine and tyrosine residues and its activity is negatively modulated by phosphatidic acid (PA). Our results reveal StCDPK2 as a signalling element involved in plant growth and development and show that its activity is tightly regulated. © 2010 Springer-Verlag. Fil:Giammaria, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grandellis, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bachmann, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gargantini, P.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Feingold, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320935_v233_n3_p593_Giammaria |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Light Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Up-Regulation Solanum tuberosum |
| spellingShingle |
Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Light Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Up-Regulation Solanum tuberosum Giammaria, V. Grandellis, C. Bachmann, S. Gargantini, P.R. Feingold, S.E. Bryan, G. Ulloa, R.M. StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling |
| topic_facet |
Autophosphorylation CDPK Light Phospholipids Solanum tuberosum calcium calcium binding protein calcium protein kinase calcium-dependent protein kinase isoprotein protein kinase recombinant protein vegetable protein amino acid sequence article chemistry enzymology gene expression regulation genetics growth, development and aging light metabolism molecular cloning molecular genetics nucleotide sequence phosphorylation potato promoter region sequence homology signal transduction upregulation Amino Acid Sequence Calcium Calcium-Binding Proteins Cloning, Molecular Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Light Molecular Sequence Data Phosphorylation Plant Proteins Promoter Regions, Genetic Protein Isoforms Protein Kinases Recombinant Proteins Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Up-Regulation Solanum tuberosum |
| description |
Calcium-dependent protein kinases (CDPKs) are essential calcium sensors. In this work, we have studied StCDPK2 isoform from potato both at gene and protein level. StCdpk2 genomic sequence contains eight exons and seven introns, as was observed for StCdpk1. There is one copy of the gene per genome located in chromosome 7. StCDPK2 encodes an active CDPK of 515 aminoacids, with an apparent MW of 57 kDa, which presents myristoylation and palmitoylation consensus in its N-terminus. StCDPK2 is highly expressed in leaves and green sprouts; enhanced expression was detected under light treatment, which corresponds well with light responsive cis-acting elements found in its promoter sequence. Antibodies against the recombinant StCDPK2::6xHis protein detected this isoform in soluble and particulate fractions from leaves. StCDPK2 autophosphorylation and kinase activity are both calcium dependent reaching half maximal activation at 0.6 μM calcium. The active kinase is autophosphorylated on serine and tyrosine residues and its activity is negatively modulated by phosphatidic acid (PA). Our results reveal StCDPK2 as a signalling element involved in plant growth and development and show that its activity is tightly regulated. © 2010 Springer-Verlag. |
| format |
JOUR |
| author |
Giammaria, V. Grandellis, C. Bachmann, S. Gargantini, P.R. Feingold, S.E. Bryan, G. Ulloa, R.M. |
| author_facet |
Giammaria, V. Grandellis, C. Bachmann, S. Gargantini, P.R. Feingold, S.E. Bryan, G. Ulloa, R.M. |
| author_sort |
Giammaria, V. |
| title |
StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling |
| title_short |
StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling |
| title_full |
StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling |
| title_fullStr |
StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling |
| title_full_unstemmed |
StCDPK2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling |
| title_sort |
stcdpk2 expression and activity reveal a highly responsive potato calcium-dependent protein kinase involved in light signalling |
| url |
http://hdl.handle.net/20.500.12110/paper_00320935_v233_n3_p593_Giammaria |
| work_keys_str_mv |
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