Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves

Tuber formation in potato (Solanum tuberosum L.) is regulated by hormonal and environmental signals that are thought to be integrated in the leaves. The molecular mechanisms that mediate the responses to tuberization-related signals in leaves remain largely unknown. In this study we analyzed the rol...

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Autores principales:	País, S.M., García, M.N.M., Téllez-Iñón, M.T., Capiati, D.A.
Formato:	JOUR
Materias:	Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00320935_v232_n1_p37_Pais
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00320935_v232_n1_p37_Pais
record_format	dspace
spelling	todo:paper_00320935_v232_n1_p37_Pais2023-10-03T14:45:02Z Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves País, S.M. García, M.N.M. Téllez-Iñón, M.T. Capiati, D.A. Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Solanum tuberosum Tuber formation in potato (Solanum tuberosum L.) is regulated by hormonal and environmental signals that are thought to be integrated in the leaves. The molecular mechanisms that mediate the responses to tuberization-related signals in leaves remain largely unknown. In this study we analyzed the roles of protein phosphatase type 2A catalytic subunits (PP2Ac) in the leaf responses to conditions that affect tuberization. The responses were monitored by analyzing the expression of the "tuber-specific" genes Patatin and Pin2, which are induced in tubers and leaves during tuber induction. Experiments using PP2A inhibitors, together with PP2Ac expression profiles under conditions that affect tuberization indicate that high sucrose/nitrogen ratio, which promotes tuber formation, increases the transcript levels of Patatin and Pin2, by increasing the activity of PP2As without affecting PP2Ac mRNA or protein levels. Gibberellic acid (GA), a negative regulator of tuberization, down-regulates the transcription of catalytic subunits of PP2As from the subfamily I and decreases their enzyme levels. In addition, GA inhibits the expression of Patatin and Pin2 possibly by a PP2A-independent mechanism. PP2Ac down-regulation by GA may inhibit tuberization signaling downstream of the inductive effects of high sucrose/nitrogen ratio. These results are consistent with the hypothesis that PP2As of the subfamily I may positively modulate the signaling pathways that lead to the transcriptional activation of "tuber-specific" genes in leaves, and act as molecular switches regulated by both positive and negative modulators of tuberization. © 2010 Springer-Verlag. Fil:País, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00320935_v232_n1_p37_Pais
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Solanum tuberosum
spellingShingle	Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Solanum tuberosum País, S.M. García, M.N.M. Téllez-Iñón, M.T. Capiati, D.A. Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves
topic_facet	Gibberellic acid Patatin Pin2 PP2A Sucrose Tuberization phosphoprotein phosphatase 2 primer DNA amino acid sequence article chemistry enzyme active site enzymology metabolism molecular genetics nucleotide sequence plant leaf potato reverse transcription polymerase chain reaction sequence homology signal transduction Western blotting Amino Acid Sequence Base Sequence Blotting, Western Catalytic Domain DNA Primers Molecular Sequence Data Plant Leaves Protein Phosphatase 2 Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Signal Transduction Solanum tuberosum Solanum tuberosum
description	Tuber formation in potato (Solanum tuberosum L.) is regulated by hormonal and environmental signals that are thought to be integrated in the leaves. The molecular mechanisms that mediate the responses to tuberization-related signals in leaves remain largely unknown. In this study we analyzed the roles of protein phosphatase type 2A catalytic subunits (PP2Ac) in the leaf responses to conditions that affect tuberization. The responses were monitored by analyzing the expression of the "tuber-specific" genes Patatin and Pin2, which are induced in tubers and leaves during tuber induction. Experiments using PP2A inhibitors, together with PP2Ac expression profiles under conditions that affect tuberization indicate that high sucrose/nitrogen ratio, which promotes tuber formation, increases the transcript levels of Patatin and Pin2, by increasing the activity of PP2As without affecting PP2Ac mRNA or protein levels. Gibberellic acid (GA), a negative regulator of tuberization, down-regulates the transcription of catalytic subunits of PP2As from the subfamily I and decreases their enzyme levels. In addition, GA inhibits the expression of Patatin and Pin2 possibly by a PP2A-independent mechanism. PP2Ac down-regulation by GA may inhibit tuberization signaling downstream of the inductive effects of high sucrose/nitrogen ratio. These results are consistent with the hypothesis that PP2As of the subfamily I may positively modulate the signaling pathways that lead to the transcriptional activation of "tuber-specific" genes in leaves, and act as molecular switches regulated by both positive and negative modulators of tuberization. © 2010 Springer-Verlag.
format	JOUR
author	País, S.M. García, M.N.M. Téllez-Iñón, M.T. Capiati, D.A.
author_facet	País, S.M. García, M.N.M. Téllez-Iñón, M.T. Capiati, D.A.
author_sort	País, S.M.
title	Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves
title_short	Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves
title_full	Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves
title_fullStr	Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves
title_full_unstemmed	Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves
title_sort	protein phosphatases type 2a mediate tuberization signaling in solanum tuberosum l. leaves
url	http://hdl.handle.net/20.500.12110/paper_00320935_v232_n1_p37_Pais
work_keys_str_mv	AT paissm proteinphosphatasestype2amediatetuberizationsignalinginsolanumtuberosumlleaves AT garciamnm proteinphosphatasestype2amediatetuberizationsignalinginsolanumtuberosumlleaves AT tellezinonmt proteinphosphatasestype2amediatetuberizationsignalinginsolanumtuberosumlleaves AT capiatida proteinphosphatasestype2amediatetuberizationsignalinginsolanumtuberosumlleaves
_version_	1807323231695142912

Protein phosphatases type 2A mediate tuberization signaling in Solanum tuberosum L. leaves

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