Biosynthesis of digitoxin in Digitalis purpurea
Simultaneous administration of a mixture of [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid and [21-14C]-3β-hydroxy-5β-pregnan-20-one to Digitalis purpurea intact plants produced double labelled digitoxin. The 3H: 14C ratio of the cardenolide was considerably higher than the 3H: 14C ratio of the...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00319422_v28_n1_p109_Deluca |
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todo:paper_00319422_v28_n1_p109_Deluca2023-10-03T14:44:07Z Biosynthesis of digitoxin in Digitalis purpurea Deluca, M.E. Seldes, A.M. Gros, E.G. biosynthesis cardenolide Digitalis purpurea digitoxin Scrophulariceae [21-14C]-3β-hydroxy-5β-pregnan-20-one. [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid Simultaneous administration of a mixture of [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid and [21-14C]-3β-hydroxy-5β-pregnan-20-one to Digitalis purpurea intact plants produced double labelled digitoxin. The 3H: 14C ratio of the cardenolide was considerably higher than the 3H: 14C ratio of the administered substrates. This result indicates that in the main biosynthetic pathway from a 20-keto-pregnane to a cardenolide the introduction of an acetate unit at C-20 should occur before the hydroxylation at C-21. © 1989. Fil:Deluca, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Seldes, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gros, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00319422_v28_n1_p109_Deluca |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
biosynthesis cardenolide Digitalis purpurea digitoxin Scrophulariceae [21-14C]-3β-hydroxy-5β-pregnan-20-one. [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid |
| spellingShingle |
biosynthesis cardenolide Digitalis purpurea digitoxin Scrophulariceae [21-14C]-3β-hydroxy-5β-pregnan-20-one. [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid Deluca, M.E. Seldes, A.M. Gros, E.G. Biosynthesis of digitoxin in Digitalis purpurea |
| topic_facet |
biosynthesis cardenolide Digitalis purpurea digitoxin Scrophulariceae [21-14C]-3β-hydroxy-5β-pregnan-20-one. [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid |
| description |
Simultaneous administration of a mixture of [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid and [21-14C]-3β-hydroxy-5β-pregnan-20-one to Digitalis purpurea intact plants produced double labelled digitoxin. The 3H: 14C ratio of the cardenolide was considerably higher than the 3H: 14C ratio of the administered substrates. This result indicates that in the main biosynthetic pathway from a 20-keto-pregnane to a cardenolide the introduction of an acetate unit at C-20 should occur before the hydroxylation at C-21. © 1989. |
| format |
JOUR |
| author |
Deluca, M.E. Seldes, A.M. Gros, E.G. |
| author_facet |
Deluca, M.E. Seldes, A.M. Gros, E.G. |
| author_sort |
Deluca, M.E. |
| title |
Biosynthesis of digitoxin in Digitalis purpurea |
| title_short |
Biosynthesis of digitoxin in Digitalis purpurea |
| title_full |
Biosynthesis of digitoxin in Digitalis purpurea |
| title_fullStr |
Biosynthesis of digitoxin in Digitalis purpurea |
| title_full_unstemmed |
Biosynthesis of digitoxin in Digitalis purpurea |
| title_sort |
biosynthesis of digitoxin in digitalis purpurea |
| url |
http://hdl.handle.net/20.500.12110/paper_00319422_v28_n1_p109_Deluca |
| work_keys_str_mv |
AT delucame biosynthesisofdigitoxinindigitalispurpurea AT seldesam biosynthesisofdigitoxinindigitalispurpurea AT groseg biosynthesisofdigitoxinindigitalispurpurea |
| _version_ |
1782030978401697792 |