Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells
The phosphorylase isoenzyme composition of soluble preparations isolated from potato (Solanum tuberosum L. cv. Spunta) tuber‐derived callus has been studied by polyacrylamide gel electrophoresis and affinity electrophoresis. Native electrophoretic profiles indicate that dedifferentiated callus tissu...
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todo:paper_00319317_v76_n2_p221_Catz2023-10-03T14:43:39Z Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells Catz, D.S. Moreno, S. Tandecarz, J.S. Callus dedifferentiation isoenzymes phosphorylase potato Solanum tuberosum The phosphorylase isoenzyme composition of soluble preparations isolated from potato (Solanum tuberosum L. cv. Spunta) tuber‐derived callus has been studied by polyacrylamide gel electrophoresis and affinity electrophoresis. Native electrophoretic profiles indicate that dedifferentiated callus tissue contains a single form of phosphorylase that differs in primer requirement, charge and affinity towards branched α‐1,4/1,6‐glucans from the major phosphorylase form (phosphorylase II) in potato tuber. This latter molecular form is missing in dedifferentiated callus. However, callus phosphorylase appears to be closely related to tuber phosphorylase I, a minor form found in the original explant tissue. Copyright © 1989, Wiley Blackwell. All rights reserved Fil:Catz, D.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Moreno, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tandecarz, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00319317_v76_n2_p221_Catz |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Callus dedifferentiation isoenzymes phosphorylase potato Solanum tuberosum |
spellingShingle |
Callus dedifferentiation isoenzymes phosphorylase potato Solanum tuberosum Catz, D.S. Moreno, S. Tandecarz, J.S. Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells |
topic_facet |
Callus dedifferentiation isoenzymes phosphorylase potato Solanum tuberosum |
description |
The phosphorylase isoenzyme composition of soluble preparations isolated from potato (Solanum tuberosum L. cv. Spunta) tuber‐derived callus has been studied by polyacrylamide gel electrophoresis and affinity electrophoresis. Native electrophoretic profiles indicate that dedifferentiated callus tissue contains a single form of phosphorylase that differs in primer requirement, charge and affinity towards branched α‐1,4/1,6‐glucans from the major phosphorylase form (phosphorylase II) in potato tuber. This latter molecular form is missing in dedifferentiated callus. However, callus phosphorylase appears to be closely related to tuber phosphorylase I, a minor form found in the original explant tissue. Copyright © 1989, Wiley Blackwell. All rights reserved |
format |
JOUR |
author |
Catz, D.S. Moreno, S. Tandecarz, J.S. |
author_facet |
Catz, D.S. Moreno, S. Tandecarz, J.S. |
author_sort |
Catz, D.S. |
title |
Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells |
title_short |
Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells |
title_full |
Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells |
title_fullStr |
Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells |
title_full_unstemmed |
Change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells |
title_sort |
change in phosphorylase isoenzymes during dedifferentiation of potato tuber cells |
url |
http://hdl.handle.net/20.500.12110/paper_00319317_v76_n2_p221_Catz |
work_keys_str_mv |
AT catzds changeinphosphorylaseisoenzymesduringdedifferentiationofpotatotubercells AT morenos changeinphosphorylaseisoenzymesduringdedifferentiationofpotatotubercells AT tandecarzjs changeinphosphorylaseisoenzymesduringdedifferentiationofpotatotubercells |
_version_ |
1782030279714537472 |