Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum

The effect of jasmonic acid (JA) on plant growth and on calcium-dependent protein kinase (CDPK) activity and expression was studied in non-photoperiodic potato plants, Solanum tuberosum L. var. Spunta, grown in vitro. Stem cuttings were grown for 45 days (long treatment, LT) in MS medium with increa...

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Autores principales: Ulloa, R.M., Raíces, M., MacIntosh, G.C., Maldonado, S., Téllez-Iñón, M.T.
Formato: JOUR
Materias:
Calcium
Cells
Enzymes
Hormones
Optical microscopy
RNA
Tissue
Transmission electron microscopy
Shoot elongation
Plants (botany)
Solanum
Solanum tuberosum
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00319317_v115_n3_p417_Ulloa
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00319317_v115_n3_p417_Ulloa2023-10-03T14:43:37Z Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum Ulloa, R.M. Raíces, M. MacIntosh, G.C. Maldonado, S. Téllez-Iñón, M.T. Calcium Cells Enzymes Hormones Optical microscopy RNA Tissue Transmission electron microscopy Shoot elongation Plants (botany) Solanum Solanum tuberosum The effect of jasmonic acid (JA) on plant growth and on calcium-dependent protein kinase (CDPK) activity and expression was studied in non-photoperiodic potato plants, Solanum tuberosum L. var. Spunta, grown in vitro. Stem cuttings were grown for 45 days (long treatment, LT) in MS medium with increasing concentrations of JA. For short treatments (ST) adult plants grown in MS were transferred for 1, 4 and 20 h to JA containing media. During the LT, low concentrations of JA promoted cell expansion and shoot elongation while higher concentrations caused growth inhibition. Under these conditions, treated plants showed root shortening and tuber formation was not induced. Morphological and histochemical studies using light microscopy and TEM analysis of leaves from treated plants revealed that JA also affected subcellular organelles of mesophyll cells. Peroxisomes increased in size and number, and an autophagic process was triggered in response to high concentrations of the hormone. CDPK activity, determined in crude extracts of treated plants (LT), was inhibited (up to 80%). Plant growth and CDPK inhibition were reverted upon transfer of the plants to hormone-free medium. Soluble CDPK activity decreased in response to JA short treatment. Concomitantly, a decline in the steady state levels of StCDPK2 mRNA, a potato CDPK isoform that is expressed in leaves, was observed. These data suggest that the phytohormone down-regulated the expression and activity of the kinase. Fil:Raíces, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00319317_v115_n3_p417_Ulloa
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Calcium
Cells
Enzymes
Hormones
Optical microscopy
RNA
Tissue
Transmission electron microscopy
Shoot elongation
Plants (botany)
Solanum
Solanum tuberosum
spellingShingle Calcium
Cells
Enzymes
Hormones
Optical microscopy
RNA
Tissue
Transmission electron microscopy
Shoot elongation
Plants (botany)
Solanum
Solanum tuberosum
Ulloa, R.M.
Raíces, M.
MacIntosh, G.C.
Maldonado, S.
Téllez-Iñón, M.T.
Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum
topic_facet Calcium
Cells
Enzymes
Hormones
Optical microscopy
RNA
Tissue
Transmission electron microscopy
Shoot elongation
Plants (botany)
Solanum
Solanum tuberosum
description The effect of jasmonic acid (JA) on plant growth and on calcium-dependent protein kinase (CDPK) activity and expression was studied in non-photoperiodic potato plants, Solanum tuberosum L. var. Spunta, grown in vitro. Stem cuttings were grown for 45 days (long treatment, LT) in MS medium with increasing concentrations of JA. For short treatments (ST) adult plants grown in MS were transferred for 1, 4 and 20 h to JA containing media. During the LT, low concentrations of JA promoted cell expansion and shoot elongation while higher concentrations caused growth inhibition. Under these conditions, treated plants showed root shortening and tuber formation was not induced. Morphological and histochemical studies using light microscopy and TEM analysis of leaves from treated plants revealed that JA also affected subcellular organelles of mesophyll cells. Peroxisomes increased in size and number, and an autophagic process was triggered in response to high concentrations of the hormone. CDPK activity, determined in crude extracts of treated plants (LT), was inhibited (up to 80%). Plant growth and CDPK inhibition were reverted upon transfer of the plants to hormone-free medium. Soluble CDPK activity decreased in response to JA short treatment. Concomitantly, a decline in the steady state levels of StCDPK2 mRNA, a potato CDPK isoform that is expressed in leaves, was observed. These data suggest that the phytohormone down-regulated the expression and activity of the kinase.
format JOUR
author Ulloa, R.M.
Raíces, M.
MacIntosh, G.C.
Maldonado, S.
Téllez-Iñón, M.T.
author_facet Ulloa, R.M.
Raíces, M.
MacIntosh, G.C.
Maldonado, S.
Téllez-Iñón, M.T.
author_sort Ulloa, R.M.
title Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum
title_short Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum
title_full Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum
title_fullStr Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum
title_full_unstemmed Jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum
title_sort jasmonic acid affects plant morphology and calcium-dependent protein kinase expression and activity in solanum tuberosum
url http://hdl.handle.net/20.500.12110/paper_00319317_v115_n3_p417_Ulloa
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