Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi

A nutritional characteristic of trypanosomatid protozoa is that in vitro they need a haem-compound as a growth factor, which is supplied as haemoglobin, haematin or haemin. Because haemin and related porphyrins are an important source of oxidative stress in biological systems, the effect of haemin o...

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Autores principales:	Ciccarelli, A., Araujo, L., Batlle, A., Lombardo, E.
Formato:	JOUR
Materias:	Antioxidant enzymes Cell transformation Haeme oxygenase Haemin Trypanosoma cruzi antioxidant ascorbate peroxidase heme oxygenase 1 hemin protozoal protein superoxide dismutase thiol trypanothione reductase amastigote article concentration (parameters) controlled study culture medium epimastigote growth rate morphology nonhuman parasite cultivation parasite development priority journal protein content protein function protein synthesis Animals Culture Media Gene Expression Heme Oxygenase-1 Hemin NADH, NADPH Oxidoreductases Oxidative Stress Peroxidases Proteins Sulfhydryl Compounds Superoxide Dismutase Time Factors Protozoa Trypanosomatidae
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00311820_v134_n7_p959_Ciccarelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00311820_v134_n7_p959_Ciccarelli
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spelling	todo:paper_00311820_v134_n7_p959_Ciccarelli2023-10-03T14:41:14Z Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi Ciccarelli, A. Araujo, L. Batlle, A. Lombardo, E. Antioxidant enzymes Cell transformation Haeme oxygenase Haemin Trypanosoma cruzi antioxidant ascorbate peroxidase heme oxygenase 1 hemin protozoal protein superoxide dismutase thiol trypanothione reductase amastigote article concentration (parameters) controlled study culture medium epimastigote growth rate morphology nonhuman parasite cultivation parasite development priority journal protein content protein function protein synthesis Trypanosoma cruzi Animals Culture Media Gene Expression Heme Oxygenase-1 Hemin NADH, NADPH Oxidoreductases Oxidative Stress Peroxidases Proteins Sulfhydryl Compounds Superoxide Dismutase Time Factors Trypanosoma cruzi Protozoa Trypanosoma cruzi Trypanosomatidae A nutritional characteristic of trypanosomatid protozoa is that in vitro they need a haem-compound as a growth factor, which is supplied as haemoglobin, haematin or haemin. Because haemin and related porphyrins are an important source of oxidative stress in biological systems, the effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi was evaluated. We have observed that, in epimastigotes grown under different haemin concentrations in the culture medium (0-30 mg/l), 5 mg/l was the haemin concentration yielding optimum growth. Above 15 mg/l there was a clear decrease in growth rate, producing the epimastigote to amastigote transformation. Such morphological change was observed together with a marked injury of the enzymatic machinery of the parasite, leading to diminished protein synthesis as well as lower activity of the antioxidant enzymes (superoxide dismutase, ascorbate peroxidase and trypanothione reductase), reduced total thiol content and a marked increase in the HaemOx-1 activity and expression. The current work demonstrates that there is a correlation between higher haemin concentrations in the culture medium and oxidative damage in the cells. Under these conditions induction of HaemOx-1 would indicate the important role of this enzyme as an antioxidant defence response in Trypanosoma cruzi. © 2007 Cambridge University Press. Fil:Ciccarelli, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Araujo, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00311820_v134_n7_p959_Ciccarelli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Antioxidant enzymes Cell transformation Haeme oxygenase Haemin Trypanosoma cruzi antioxidant ascorbate peroxidase heme oxygenase 1 hemin protozoal protein superoxide dismutase thiol trypanothione reductase amastigote article concentration (parameters) controlled study culture medium epimastigote growth rate morphology nonhuman parasite cultivation parasite development priority journal protein content protein function protein synthesis Trypanosoma cruzi Animals Culture Media Gene Expression Heme Oxygenase-1 Hemin NADH, NADPH Oxidoreductases Oxidative Stress Peroxidases Proteins Sulfhydryl Compounds Superoxide Dismutase Time Factors Trypanosoma cruzi Protozoa Trypanosoma cruzi Trypanosomatidae
spellingShingle	Antioxidant enzymes Cell transformation Haeme oxygenase Haemin Trypanosoma cruzi antioxidant ascorbate peroxidase heme oxygenase 1 hemin protozoal protein superoxide dismutase thiol trypanothione reductase amastigote article concentration (parameters) controlled study culture medium epimastigote growth rate morphology nonhuman parasite cultivation parasite development priority journal protein content protein function protein synthesis Trypanosoma cruzi Animals Culture Media Gene Expression Heme Oxygenase-1 Hemin NADH, NADPH Oxidoreductases Oxidative Stress Peroxidases Proteins Sulfhydryl Compounds Superoxide Dismutase Time Factors Trypanosoma cruzi Protozoa Trypanosoma cruzi Trypanosomatidae Ciccarelli, A. Araujo, L. Batlle, A. Lombardo, E. Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi
topic_facet	Antioxidant enzymes Cell transformation Haeme oxygenase Haemin Trypanosoma cruzi antioxidant ascorbate peroxidase heme oxygenase 1 hemin protozoal protein superoxide dismutase thiol trypanothione reductase amastigote article concentration (parameters) controlled study culture medium epimastigote growth rate morphology nonhuman parasite cultivation parasite development priority journal protein content protein function protein synthesis Trypanosoma cruzi Animals Culture Media Gene Expression Heme Oxygenase-1 Hemin NADH, NADPH Oxidoreductases Oxidative Stress Peroxidases Proteins Sulfhydryl Compounds Superoxide Dismutase Time Factors Trypanosoma cruzi Protozoa Trypanosoma cruzi Trypanosomatidae
description	A nutritional characteristic of trypanosomatid protozoa is that in vitro they need a haem-compound as a growth factor, which is supplied as haemoglobin, haematin or haemin. Because haemin and related porphyrins are an important source of oxidative stress in biological systems, the effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi was evaluated. We have observed that, in epimastigotes grown under different haemin concentrations in the culture medium (0-30 mg/l), 5 mg/l was the haemin concentration yielding optimum growth. Above 15 mg/l there was a clear decrease in growth rate, producing the epimastigote to amastigote transformation. Such morphological change was observed together with a marked injury of the enzymatic machinery of the parasite, leading to diminished protein synthesis as well as lower activity of the antioxidant enzymes (superoxide dismutase, ascorbate peroxidase and trypanothione reductase), reduced total thiol content and a marked increase in the HaemOx-1 activity and expression. The current work demonstrates that there is a correlation between higher haemin concentrations in the culture medium and oxidative damage in the cells. Under these conditions induction of HaemOx-1 would indicate the important role of this enzyme as an antioxidant defence response in Trypanosoma cruzi. © 2007 Cambridge University Press.
format	JOUR
author	Ciccarelli, A. Araujo, L. Batlle, A. Lombardo, E.
author_facet	Ciccarelli, A. Araujo, L. Batlle, A. Lombardo, E.
author_sort	Ciccarelli, A.
title	Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi
title_short	Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi
title_full	Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi
title_fullStr	Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi
title_full_unstemmed	Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi
title_sort	effect of haemin on growth, protein content and the antioxidant defence system in trypanosoma cruzi
url	http://hdl.handle.net/20.500.12110/paper_00311820_v134_n7_p959_Ciccarelli
work_keys_str_mv	AT ciccarellia effectofhaeminongrowthproteincontentandtheantioxidantdefencesystemintrypanosomacruzi AT araujol effectofhaeminongrowthproteincontentandtheantioxidantdefencesystemintrypanosomacruzi AT batllea effectofhaeminongrowthproteincontentandtheantioxidantdefencesystemintrypanosomacruzi AT lombardoe effectofhaeminongrowthproteincontentandtheantioxidantdefencesystemintrypanosomacruzi
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Effect of haemin on growth, protein content and the antioxidant defence system in Trypanosoma cruzi

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