Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics

A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this c...

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Autores principales: Torga, J.R., Etcheverry, J.I., Marconi, M.C.
Formato: JOUR
Materias:
Brownian dynamics
Fluorescence anisotropy
Rotational depolarization
Brownian movement
Fluorescence
Molecular dynamics
Laser pulses
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00304018_v143_n4-6_p230_Torga
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00304018_v143_n4-6_p230_Torga
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spelling todo:paper_00304018_v143_n4-6_p230_Torga2023-10-03T14:40:02Z Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics Torga, J.R. Etcheverry, J.I. Marconi, M.C. Brownian dynamics Fluorescence anisotropy Rotational depolarization Brownian movement Fluorescence Molecular dynamics Rotational depolarization Laser pulses A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors. © 1997 Elsevier Science B.V. Fil:Torga, J.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Etcheverry, J.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Marconi, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00304018_v143_n4-6_p230_Torga
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Brownian dynamics
Fluorescence anisotropy
Rotational depolarization
Brownian movement
Fluorescence
Molecular dynamics
Rotational depolarization
Laser pulses
spellingShingle Brownian dynamics
Fluorescence anisotropy
Rotational depolarization
Brownian movement
Fluorescence
Molecular dynamics
Rotational depolarization
Laser pulses
Torga, J.R.
Etcheverry, J.I.
Marconi, M.C.
Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
topic_facet Brownian dynamics
Fluorescence anisotropy
Rotational depolarization
Brownian movement
Fluorescence
Molecular dynamics
Rotational depolarization
Laser pulses
description A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors. © 1997 Elsevier Science B.V.
format JOUR
author Torga, J.R.
Etcheverry, J.I.
Marconi, M.C.
author_facet Torga, J.R.
Etcheverry, J.I.
Marconi, M.C.
author_sort Torga, J.R.
title Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_short Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_full Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_fullStr Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_full_unstemmed Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics
title_sort design of a fluorescence technique using double laser pulse excitation for the measurement of molecular brownian dynamics
url http://hdl.handle.net/20.500.12110/paper_00304018_v143_n4-6_p230_Torga
work_keys_str_mv AT torgajr designofafluorescencetechniqueusingdoublelaserpulseexcitationforthemeasurementofmolecularbrowniandynamics
AT etcheverryji designofafluorescencetechniqueusingdoublelaserpulseexcitationforthemeasurementofmolecularbrowniandynamics
AT marconimc designofafluorescencetechniqueusingdoublelaserpulseexcitationforthemeasurementofmolecularbrowniandynamics
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