Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism
Cryptochromes are widespread blue-light absorbing flavoproteins with important signaling roles. In plants they mediate de-etiolation, developmental and stress responses resulting from interaction with downstream signaling partners such as transcription factors and components of the proteasome. Recen...
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todo:paper_0028646X_v206_n4_p1450_Consentino2023-10-03T14:39:09Z Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism Consentino, L. Lambert, S. Martino, C. Jourdan, N. Bouchet, P..-E. Witczak, J. Castello, P. El-Esawi, M. Corbineau, F. d'Harlingue, A. Ahmad, M. Arabidopsis thaliana Cryptochrome Oxidative stress Photomorphogenesis Photoreceptor Reactive oxygen species (ROS) signaling bioassay dicotyledon gene expression light effect morphogenesis oxygen photoreception physiological response signaling Arabidopsis Arabidopsis thaliana Hexapoda Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome oxygen reactive oxygen metabolite Arabidopsis cell death cell fractionation cell survival drug effects evolution genetic recombination genetics light metabolism protoplast radiation response SF9 cell line signal transduction Arabidopsis Arabidopsis Proteins Biological Evolution Cell Death Cell Survival Cryptochromes Light Oxygen Protoplasts Reactive Oxygen Species Recombination, Genetic Sf9 Cells Signal Transduction Subcellular Fractions Cryptochromes are widespread blue-light absorbing flavoproteins with important signaling roles. In plants they mediate de-etiolation, developmental and stress responses resulting from interaction with downstream signaling partners such as transcription factors and components of the proteasome. Recently, it has been shown that Arabidopsis cry1 activation by blue light also results in direct enzymatic conversion of molecular oxygen (O<inf>2</inf>) to reactive oxygen species (ROS) and hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>) in vitro. Here we explored whether direct enzymatic synthesis of ROS by Arabidopsis cry1 can play a physiological role in vivo. ROS formation resulting from cry1 expression was measured by fluorescence assay in insect cell cultures and in Arabidopsis protoplasts from cryptochrome mutant seedlings. Cell death was determined by colorimetric assay. We found that ROS formation results from cry1 activation and induces cell death in insect cell cultures. In plant protoplasts, cryptochrome activation results in rapid increase in ROS formation and cell death. We conclude that ROS formation by cryptochromes may indeed be of physiological relevance and could represent a novel paradigm for cryptochrome signaling. © 2015 New Phytologist Trust. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0028646X_v206_n4_p1450_Consentino |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arabidopsis thaliana Cryptochrome Oxidative stress Photomorphogenesis Photoreceptor Reactive oxygen species (ROS) signaling bioassay dicotyledon gene expression light effect morphogenesis oxygen photoreception physiological response signaling Arabidopsis Arabidopsis thaliana Hexapoda Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome oxygen reactive oxygen metabolite Arabidopsis cell death cell fractionation cell survival drug effects evolution genetic recombination genetics light metabolism protoplast radiation response SF9 cell line signal transduction Arabidopsis Arabidopsis Proteins Biological Evolution Cell Death Cell Survival Cryptochromes Light Oxygen Protoplasts Reactive Oxygen Species Recombination, Genetic Sf9 Cells Signal Transduction Subcellular Fractions |
| spellingShingle |
Arabidopsis thaliana Cryptochrome Oxidative stress Photomorphogenesis Photoreceptor Reactive oxygen species (ROS) signaling bioassay dicotyledon gene expression light effect morphogenesis oxygen photoreception physiological response signaling Arabidopsis Arabidopsis thaliana Hexapoda Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome oxygen reactive oxygen metabolite Arabidopsis cell death cell fractionation cell survival drug effects evolution genetic recombination genetics light metabolism protoplast radiation response SF9 cell line signal transduction Arabidopsis Arabidopsis Proteins Biological Evolution Cell Death Cell Survival Cryptochromes Light Oxygen Protoplasts Reactive Oxygen Species Recombination, Genetic Sf9 Cells Signal Transduction Subcellular Fractions Consentino, L. Lambert, S. Martino, C. Jourdan, N. Bouchet, P..-E. Witczak, J. Castello, P. El-Esawi, M. Corbineau, F. d'Harlingue, A. Ahmad, M. Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism |
| topic_facet |
Arabidopsis thaliana Cryptochrome Oxidative stress Photomorphogenesis Photoreceptor Reactive oxygen species (ROS) signaling bioassay dicotyledon gene expression light effect morphogenesis oxygen photoreception physiological response signaling Arabidopsis Arabidopsis thaliana Hexapoda Arabidopsis protein CRY1 protein, Arabidopsis cryptochrome oxygen reactive oxygen metabolite Arabidopsis cell death cell fractionation cell survival drug effects evolution genetic recombination genetics light metabolism protoplast radiation response SF9 cell line signal transduction Arabidopsis Arabidopsis Proteins Biological Evolution Cell Death Cell Survival Cryptochromes Light Oxygen Protoplasts Reactive Oxygen Species Recombination, Genetic Sf9 Cells Signal Transduction Subcellular Fractions |
| description |
Cryptochromes are widespread blue-light absorbing flavoproteins with important signaling roles. In plants they mediate de-etiolation, developmental and stress responses resulting from interaction with downstream signaling partners such as transcription factors and components of the proteasome. Recently, it has been shown that Arabidopsis cry1 activation by blue light also results in direct enzymatic conversion of molecular oxygen (O<inf>2</inf>) to reactive oxygen species (ROS) and hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>) in vitro. Here we explored whether direct enzymatic synthesis of ROS by Arabidopsis cry1 can play a physiological role in vivo. ROS formation resulting from cry1 expression was measured by fluorescence assay in insect cell cultures and in Arabidopsis protoplasts from cryptochrome mutant seedlings. Cell death was determined by colorimetric assay. We found that ROS formation results from cry1 activation and induces cell death in insect cell cultures. In plant protoplasts, cryptochrome activation results in rapid increase in ROS formation and cell death. We conclude that ROS formation by cryptochromes may indeed be of physiological relevance and could represent a novel paradigm for cryptochrome signaling. © 2015 New Phytologist Trust. |
| format |
JOUR |
| author |
Consentino, L. Lambert, S. Martino, C. Jourdan, N. Bouchet, P..-E. Witczak, J. Castello, P. El-Esawi, M. Corbineau, F. d'Harlingue, A. Ahmad, M. |
| author_facet |
Consentino, L. Lambert, S. Martino, C. Jourdan, N. Bouchet, P..-E. Witczak, J. Castello, P. El-Esawi, M. Corbineau, F. d'Harlingue, A. Ahmad, M. |
| author_sort |
Consentino, L. |
| title |
Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism |
| title_short |
Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism |
| title_full |
Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism |
| title_fullStr |
Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism |
| title_full_unstemmed |
Blue-light dependent reactive oxygen species formation by Arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism |
| title_sort |
blue-light dependent reactive oxygen species formation by arabidopsis cryptochrome may define a novel evolutionarily conserved signaling mechanism |
| url |
http://hdl.handle.net/20.500.12110/paper_0028646X_v206_n4_p1450_Consentino |
| work_keys_str_mv |
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