Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus
Preincubation of synaptosome-rich homogenates of rat hypothalamus with pentoxifylline [3,7-dimethyl-1(5-oxo-hexyl)-xanthine] or theophylline resulted in significant decreases of norepinephrine, dopamine, serotonin and glutamate uptake. Methylxanthine inhibition was non-competitive; apparent Ki'...
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todo:paper_00283908_v16_n11_p785_Cardinali2023-10-03T14:39:02Z Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus Cardinali, D.P. adenosine-3',5'-monophosphate amino acid uptake dopamine uptake and release hypothalamic synaptosomes l-glutamate uptake and release methylxanthines norepinephrine uptake and release pentoxifylline [3,7-dimethyl-1-(5-oxo-hexyl)-xanthine] serotonin uptake and release theophylline bucladesine dopamine glutamic acid methylxanthine neurotransmitter noradrenalin pentoxifylline potassium radioisotope serotonin theophylline dopamine h 3 dose response drug comparison drug response glutamic acid h 3 hypothalamus in vitro study michaelis constant noradrenalin h 3 rat serotonin h 3 synaptosome theoretical study Animal Bucladesine Dopamine Glutamates Hypothalamus Male Neurotransmitters Norepinephrine Pentoxifylline Potassium Rats Serotonin Synaptosomes Theobromine Theophylline Xanthines Preincubation of synaptosome-rich homogenates of rat hypothalamus with pentoxifylline [3,7-dimethyl-1(5-oxo-hexyl)-xanthine] or theophylline resulted in significant decreases of norepinephrine, dopamine, serotonin and glutamate uptake. Methylxanthine inhibition was non-competitive; apparent Ki's ± S.E.M. for pentoxifylline and theophylline were 0.48 ± 0.03 and 0.84 ± 0.10 mM (norepinephrine), 0.20 ± 0.02 and 0.35 ± 0.05 mM (dopamine), 0.22 ± 0.03 and 0.98 ± 0.12 mM (serotonin) and 0.69 ± 0.08 and 1.13 ± 0.14 mM (glutamate). Methylxanthines did not affect the synaptosomal uptake of leucine, phenylalanine or alanine. Transmitter release evoked by increasing [K+] in the medium was augmented by pentoxifylline and theophylline in a dose-dependent manner. Dibutyryl cyclic AMP (1.0 mM) caused a significant increase of transmitter release following elevation of [K + ] in the medium but did not affect neurotransmitter uptake by hypothalamic synaptosomes. © 1977. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00283908_v16_n11_p785_Cardinali |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
adenosine-3',5'-monophosphate amino acid uptake dopamine uptake and release hypothalamic synaptosomes l-glutamate uptake and release methylxanthines norepinephrine uptake and release pentoxifylline [3,7-dimethyl-1-(5-oxo-hexyl)-xanthine] serotonin uptake and release theophylline bucladesine dopamine glutamic acid methylxanthine neurotransmitter noradrenalin pentoxifylline potassium radioisotope serotonin theophylline dopamine h 3 dose response drug comparison drug response glutamic acid h 3 hypothalamus in vitro study michaelis constant noradrenalin h 3 rat serotonin h 3 synaptosome theoretical study Animal Bucladesine Dopamine Glutamates Hypothalamus Male Neurotransmitters Norepinephrine Pentoxifylline Potassium Rats Serotonin Synaptosomes Theobromine Theophylline Xanthines |
| spellingShingle |
adenosine-3',5'-monophosphate amino acid uptake dopamine uptake and release hypothalamic synaptosomes l-glutamate uptake and release methylxanthines norepinephrine uptake and release pentoxifylline [3,7-dimethyl-1-(5-oxo-hexyl)-xanthine] serotonin uptake and release theophylline bucladesine dopamine glutamic acid methylxanthine neurotransmitter noradrenalin pentoxifylline potassium radioisotope serotonin theophylline dopamine h 3 dose response drug comparison drug response glutamic acid h 3 hypothalamus in vitro study michaelis constant noradrenalin h 3 rat serotonin h 3 synaptosome theoretical study Animal Bucladesine Dopamine Glutamates Hypothalamus Male Neurotransmitters Norepinephrine Pentoxifylline Potassium Rats Serotonin Synaptosomes Theobromine Theophylline Xanthines Cardinali, D.P. Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus |
| topic_facet |
adenosine-3',5'-monophosphate amino acid uptake dopamine uptake and release hypothalamic synaptosomes l-glutamate uptake and release methylxanthines norepinephrine uptake and release pentoxifylline [3,7-dimethyl-1-(5-oxo-hexyl)-xanthine] serotonin uptake and release theophylline bucladesine dopamine glutamic acid methylxanthine neurotransmitter noradrenalin pentoxifylline potassium radioisotope serotonin theophylline dopamine h 3 dose response drug comparison drug response glutamic acid h 3 hypothalamus in vitro study michaelis constant noradrenalin h 3 rat serotonin h 3 synaptosome theoretical study Animal Bucladesine Dopamine Glutamates Hypothalamus Male Neurotransmitters Norepinephrine Pentoxifylline Potassium Rats Serotonin Synaptosomes Theobromine Theophylline Xanthines |
| description |
Preincubation of synaptosome-rich homogenates of rat hypothalamus with pentoxifylline [3,7-dimethyl-1(5-oxo-hexyl)-xanthine] or theophylline resulted in significant decreases of norepinephrine, dopamine, serotonin and glutamate uptake. Methylxanthine inhibition was non-competitive; apparent Ki's ± S.E.M. for pentoxifylline and theophylline were 0.48 ± 0.03 and 0.84 ± 0.10 mM (norepinephrine), 0.20 ± 0.02 and 0.35 ± 0.05 mM (dopamine), 0.22 ± 0.03 and 0.98 ± 0.12 mM (serotonin) and 0.69 ± 0.08 and 1.13 ± 0.14 mM (glutamate). Methylxanthines did not affect the synaptosomal uptake of leucine, phenylalanine or alanine. Transmitter release evoked by increasing [K+] in the medium was augmented by pentoxifylline and theophylline in a dose-dependent manner. Dibutyryl cyclic AMP (1.0 mM) caused a significant increase of transmitter release following elevation of [K + ] in the medium but did not affect neurotransmitter uptake by hypothalamic synaptosomes. © 1977. |
| format |
JOUR |
| author |
Cardinali, D.P. |
| author_facet |
Cardinali, D.P. |
| author_sort |
Cardinali, D.P. |
| title |
Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus |
| title_short |
Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus |
| title_full |
Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus |
| title_fullStr |
Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus |
| title_full_unstemmed |
Effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus |
| title_sort |
effects of pentoxifylline and theophylline on neurotransmitter uptake and release by synaptosome-rich homogenates of the rat hypothalamus |
| url |
http://hdl.handle.net/20.500.12110/paper_00283908_v16_n11_p785_Cardinali |
| work_keys_str_mv |
AT cardinalidp effectsofpentoxifyllineandtheophyllineonneurotransmitteruptakeandreleasebysynaptosomerichhomogenatesoftherathypothalamus |
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1807320315120844800 |