Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster
We previously reported the presence of serotonin (5-HT) in testes from golden hamster, a photoperiodic species which is a useful model for the study of states of male (in)fertility. The aims of this study were to investigate: (1) the presence of intrinsic sources of 5-HT in the testis; (2) the role...
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todo:paper_00283835_v76_n1_p35_Frungieri2023-10-03T14:38:57Z Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster Frungieri, M.B. Zitta, K. Pignataro, O.P. Gonzalez-Calvar, S.I. Calandra, R.S. Catecholamines Corticotropin-releasing hormone Gonadal steroids Hamster Immunocytochemistry Serotonin Serotonin agonists Serotonin antagonists Steroidogenesis Testis 2 dipropylamino 8 hydroxytetralin 4 iodo 2,5 dimethoxyamphetamine 4 iodo n [2 [4 (methoxyphenyl) 1 piperazinyl]ethyl] n 2 pyridinylbenzamide adrenalin alpha 1 adrenergic receptor androstanediol androstanolone benzamide derivative beta 1 adrenergic receptor bucladesine chorionic gonadotropin clozapine corticotropin releasing factor[9-41] cyclic AMP isoprenaline ketanserin metitepine metoclopramide noradrenalin oxymetazoline phenylephrine prazosin quipazine serotonin serotonin 1A receptor serotonin 2A receptor testosterone tryptophan hydroxylase unclassified drug unindexed drug zinterol androgen synthesis animal tissue article catecholaminergic system chromatin controlled study dose response drug effect hamster immunohistochemistry Leydig cell male nonhuman photoperiodicity prepuberty priority journal serotoninergic system steroidogenesis testis Animals Bucladesine Catecholamines Chorionic Gonadotropin Corticotropin-Releasing Hormone Cricetinae Cyclic AMP Drug Interactions Epinephrine Immunohistochemistry Leydig Cells Male Mesocricetus Norepinephrine Peptide Fragments Photoperiod Receptor, Serotonin, 5-HT2A Receptors, Adrenergic, alpha-1 Receptors, Adrenergic, beta-1 Receptors, Serotonin Serotonin Serotonin Antagonists Testis Testosterone Tryptophan Hydroxylase We previously reported the presence of serotonin (5-HT) in testes from golden hamster, a photoperiodic species which is a useful model for the study of states of male (in)fertility. The aims of this study were to investigate: (1) the presence of intrinsic sources of 5-HT in the testis; (2) the role of 5-HT in in vitro androgen production; (3) the serotoninergic receptor subtypes in the testis, and (4) the existence of interactions among the 5-HT receptors and the testicular catecholaminergic and corticotropin-releasing hormone (CRH) systems. Immunohistochemical studies revealed the presence of tryptophan hydroxylase, a 5-HT-biosynthetic enzyme, in interstitial cells which show the characteristic punctate chromatin pattern of Leydig cells. We describe an inhibitory action of 5-HT on testosterone, dihydrotestosterone, and androstane-3α,17β-diol production from testes of peripubertal and adult hamsters maintained in a long photoperiod (14/10 h light/dark), and adult animals exposed to a short photoperiod (6/18 h light/dark). By using several agonists and antagonists of 5-HT receptors, we characterized 5-HT1A and 5-HT2A receptor subtypes involved in the inhibitory action of this neurotransmitter on human chorionic gonadotropin stimulated cyclic adenosine monophosphate and testosterone production. CRH also produced a negative modulation of both parameters, but epinephrine and norepinephrine, through α1/β1-adrenergic receptors, exerted a stimulatory action. 5-HT1A, 5-HT2, and CRH antagonists showed that the testicular activity of the serotoninergic system, but also the α1/β1-adrenergic receptor system, is mediated by CRH. Moreover, interactions between the 5-HT2A receptor system and α1/β-adrenergic receptors have been established. Thus, these data suggest that α1/β1-adrenergic receptors are involved in the local regulatory action exerted by 5-HT on steroidogenesis through a 5-HT2-receptor-mediated response and the CRH system. Copyright © 2002 S. Karger AG, Basel. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00283835_v76_n1_p35_Frungieri |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Catecholamines Corticotropin-releasing hormone Gonadal steroids Hamster Immunocytochemistry Serotonin Serotonin agonists Serotonin antagonists Steroidogenesis Testis 2 dipropylamino 8 hydroxytetralin 4 iodo 2,5 dimethoxyamphetamine 4 iodo n [2 [4 (methoxyphenyl) 1 piperazinyl]ethyl] n 2 pyridinylbenzamide adrenalin alpha 1 adrenergic receptor androstanediol androstanolone benzamide derivative beta 1 adrenergic receptor bucladesine chorionic gonadotropin clozapine corticotropin releasing factor[9-41] cyclic AMP isoprenaline ketanserin metitepine metoclopramide noradrenalin oxymetazoline phenylephrine prazosin quipazine serotonin serotonin 1A receptor serotonin 2A receptor testosterone tryptophan hydroxylase unclassified drug unindexed drug zinterol androgen synthesis animal tissue article catecholaminergic system chromatin controlled study dose response drug effect hamster immunohistochemistry Leydig cell male nonhuman photoperiodicity prepuberty priority journal serotoninergic system steroidogenesis testis Animals Bucladesine Catecholamines Chorionic Gonadotropin Corticotropin-Releasing Hormone Cricetinae Cyclic AMP Drug Interactions Epinephrine Immunohistochemistry Leydig Cells Male Mesocricetus Norepinephrine Peptide Fragments Photoperiod Receptor, Serotonin, 5-HT2A Receptors, Adrenergic, alpha-1 Receptors, Adrenergic, beta-1 Receptors, Serotonin Serotonin Serotonin Antagonists Testis Testosterone Tryptophan Hydroxylase |
| spellingShingle |
Catecholamines Corticotropin-releasing hormone Gonadal steroids Hamster Immunocytochemistry Serotonin Serotonin agonists Serotonin antagonists Steroidogenesis Testis 2 dipropylamino 8 hydroxytetralin 4 iodo 2,5 dimethoxyamphetamine 4 iodo n [2 [4 (methoxyphenyl) 1 piperazinyl]ethyl] n 2 pyridinylbenzamide adrenalin alpha 1 adrenergic receptor androstanediol androstanolone benzamide derivative beta 1 adrenergic receptor bucladesine chorionic gonadotropin clozapine corticotropin releasing factor[9-41] cyclic AMP isoprenaline ketanserin metitepine metoclopramide noradrenalin oxymetazoline phenylephrine prazosin quipazine serotonin serotonin 1A receptor serotonin 2A receptor testosterone tryptophan hydroxylase unclassified drug unindexed drug zinterol androgen synthesis animal tissue article catecholaminergic system chromatin controlled study dose response drug effect hamster immunohistochemistry Leydig cell male nonhuman photoperiodicity prepuberty priority journal serotoninergic system steroidogenesis testis Animals Bucladesine Catecholamines Chorionic Gonadotropin Corticotropin-Releasing Hormone Cricetinae Cyclic AMP Drug Interactions Epinephrine Immunohistochemistry Leydig Cells Male Mesocricetus Norepinephrine Peptide Fragments Photoperiod Receptor, Serotonin, 5-HT2A Receptors, Adrenergic, alpha-1 Receptors, Adrenergic, beta-1 Receptors, Serotonin Serotonin Serotonin Antagonists Testis Testosterone Tryptophan Hydroxylase Frungieri, M.B. Zitta, K. Pignataro, O.P. Gonzalez-Calvar, S.I. Calandra, R.S. Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster |
| topic_facet |
Catecholamines Corticotropin-releasing hormone Gonadal steroids Hamster Immunocytochemistry Serotonin Serotonin agonists Serotonin antagonists Steroidogenesis Testis 2 dipropylamino 8 hydroxytetralin 4 iodo 2,5 dimethoxyamphetamine 4 iodo n [2 [4 (methoxyphenyl) 1 piperazinyl]ethyl] n 2 pyridinylbenzamide adrenalin alpha 1 adrenergic receptor androstanediol androstanolone benzamide derivative beta 1 adrenergic receptor bucladesine chorionic gonadotropin clozapine corticotropin releasing factor[9-41] cyclic AMP isoprenaline ketanserin metitepine metoclopramide noradrenalin oxymetazoline phenylephrine prazosin quipazine serotonin serotonin 1A receptor serotonin 2A receptor testosterone tryptophan hydroxylase unclassified drug unindexed drug zinterol androgen synthesis animal tissue article catecholaminergic system chromatin controlled study dose response drug effect hamster immunohistochemistry Leydig cell male nonhuman photoperiodicity prepuberty priority journal serotoninergic system steroidogenesis testis Animals Bucladesine Catecholamines Chorionic Gonadotropin Corticotropin-Releasing Hormone Cricetinae Cyclic AMP Drug Interactions Epinephrine Immunohistochemistry Leydig Cells Male Mesocricetus Norepinephrine Peptide Fragments Photoperiod Receptor, Serotonin, 5-HT2A Receptors, Adrenergic, alpha-1 Receptors, Adrenergic, beta-1 Receptors, Serotonin Serotonin Serotonin Antagonists Testis Testosterone Tryptophan Hydroxylase |
| description |
We previously reported the presence of serotonin (5-HT) in testes from golden hamster, a photoperiodic species which is a useful model for the study of states of male (in)fertility. The aims of this study were to investigate: (1) the presence of intrinsic sources of 5-HT in the testis; (2) the role of 5-HT in in vitro androgen production; (3) the serotoninergic receptor subtypes in the testis, and (4) the existence of interactions among the 5-HT receptors and the testicular catecholaminergic and corticotropin-releasing hormone (CRH) systems. Immunohistochemical studies revealed the presence of tryptophan hydroxylase, a 5-HT-biosynthetic enzyme, in interstitial cells which show the characteristic punctate chromatin pattern of Leydig cells. We describe an inhibitory action of 5-HT on testosterone, dihydrotestosterone, and androstane-3α,17β-diol production from testes of peripubertal and adult hamsters maintained in a long photoperiod (14/10 h light/dark), and adult animals exposed to a short photoperiod (6/18 h light/dark). By using several agonists and antagonists of 5-HT receptors, we characterized 5-HT1A and 5-HT2A receptor subtypes involved in the inhibitory action of this neurotransmitter on human chorionic gonadotropin stimulated cyclic adenosine monophosphate and testosterone production. CRH also produced a negative modulation of both parameters, but epinephrine and norepinephrine, through α1/β1-adrenergic receptors, exerted a stimulatory action. 5-HT1A, 5-HT2, and CRH antagonists showed that the testicular activity of the serotoninergic system, but also the α1/β1-adrenergic receptor system, is mediated by CRH. Moreover, interactions between the 5-HT2A receptor system and α1/β-adrenergic receptors have been established. Thus, these data suggest that α1/β1-adrenergic receptors are involved in the local regulatory action exerted by 5-HT on steroidogenesis through a 5-HT2-receptor-mediated response and the CRH system. Copyright © 2002 S. Karger AG, Basel. |
| format |
JOUR |
| author |
Frungieri, M.B. Zitta, K. Pignataro, O.P. Gonzalez-Calvar, S.I. Calandra, R.S. |
| author_facet |
Frungieri, M.B. Zitta, K. Pignataro, O.P. Gonzalez-Calvar, S.I. Calandra, R.S. |
| author_sort |
Frungieri, M.B. |
| title |
Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster |
| title_short |
Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster |
| title_full |
Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster |
| title_fullStr |
Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster |
| title_full_unstemmed |
Interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating cAMP and testosterone production in the golden hamster |
| title_sort |
interactions between testicular serotoninergic, catecholaminergic, and corticotropin-releasing hormone systems modulating camp and testosterone production in the golden hamster |
| url |
http://hdl.handle.net/20.500.12110/paper_00283835_v76_n1_p35_Frungieri |
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