Corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects

Stereoselective competition was used to determine (3H)-aldosterone binding to type I corticosteroid receptors, and (3H)-dexamethasone binding to type II receptors in punches obtained from 11 brain regions of short-term adrenalectomized (ADX) rats. It was observed that type I receptor binding was alm...

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Autores principales: Magarinos, A.M., Ferrini, M., De Nicola, A.F.
Formato: JOUR
Materias:
aldosterone
corticosteroid receptor
corticosterone
dexamethasone
radioisotope
animal cell
hippocampus
hypothalamus
male
nonhuman
priority journal
rat
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00283835_v50_n6_p673_Magarinos
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00283835_v50_n6_p673_Magarinos
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spelling todo:paper_00283835_v50_n6_p673_Magarinos2023-10-03T14:38:52Z Corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects Magarinos, A.M. Ferrini, M. De Nicola, A.F. aldosterone corticosteroid receptor corticosterone dexamethasone radioisotope animal cell hippocampus hypothalamus male nonhuman priority journal rat Stereoselective competition was used to determine (3H)-aldosterone binding to type I corticosteroid receptors, and (3H)-dexamethasone binding to type II receptors in punches obtained from 11 brain regions of short-term adrenalectomized (ADX) rats. It was observed that type I receptor binding was almost exclusive of the hippocampus (HIPPO), while type II receptor binding was more generally distributed among HIPPO, cerebral cortex, lateral septum, ventromedial and arcuate hypothalamic nuclei, with lower levels in 6 additional regions studied. We determined corticosterone (CORT) in brain punches from ADX rats, ADX rats receiving CORT for 5 days, intact rats and intact rats receiving ACTH for 5 days. We correlated (3H)-ligand binding with CORT content in punches obtained from identical brain regions and showed a significant positive correlation in the case of the ADX plus CORT group, for type II corticosteroid receptors. Similarly, a significant correlation emerged with type II sites, when binding capacity was correlated with percentage increases of CORT in brain areas of rats receiving ACTH. It is suggested that in situations where CORT levels are elevated, changes in CORT retention throughout the brain occur as a function of the type II glucocorticoid receptor, although at the level of the HIPPO, both receptors may provide appropriate control of the CNS-pituitary-adrenal axis, according to the physiological or stress levels of circulating hormone. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00283835_v50_n6_p673_Magarinos
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic aldosterone
corticosteroid receptor
corticosterone
dexamethasone
radioisotope
animal cell
hippocampus
hypothalamus
male
nonhuman
priority journal
rat
spellingShingle aldosterone
corticosteroid receptor
corticosterone
dexamethasone
radioisotope
animal cell
hippocampus
hypothalamus
male
nonhuman
priority journal
rat
Magarinos, A.M.
Ferrini, M.
De Nicola, A.F.
Corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects
topic_facet aldosterone
corticosteroid receptor
corticosterone
dexamethasone
radioisotope
animal cell
hippocampus
hypothalamus
male
nonhuman
priority journal
rat
description Stereoselective competition was used to determine (3H)-aldosterone binding to type I corticosteroid receptors, and (3H)-dexamethasone binding to type II receptors in punches obtained from 11 brain regions of short-term adrenalectomized (ADX) rats. It was observed that type I receptor binding was almost exclusive of the hippocampus (HIPPO), while type II receptor binding was more generally distributed among HIPPO, cerebral cortex, lateral septum, ventromedial and arcuate hypothalamic nuclei, with lower levels in 6 additional regions studied. We determined corticosterone (CORT) in brain punches from ADX rats, ADX rats receiving CORT for 5 days, intact rats and intact rats receiving ACTH for 5 days. We correlated (3H)-ligand binding with CORT content in punches obtained from identical brain regions and showed a significant positive correlation in the case of the ADX plus CORT group, for type II corticosteroid receptors. Similarly, a significant correlation emerged with type II sites, when binding capacity was correlated with percentage increases of CORT in brain areas of rats receiving ACTH. It is suggested that in situations where CORT levels are elevated, changes in CORT retention throughout the brain occur as a function of the type II glucocorticoid receptor, although at the level of the HIPPO, both receptors may provide appropriate control of the CNS-pituitary-adrenal axis, according to the physiological or stress levels of circulating hormone.
format JOUR
author Magarinos, A.M.
Ferrini, M.
De Nicola, A.F.
author_facet Magarinos, A.M.
Ferrini, M.
De Nicola, A.F.
author_sort Magarinos, A.M.
title Corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects
title_short Corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects
title_full Corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects
title_fullStr Corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects
title_full_unstemmed Corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects
title_sort corticosteroid receptors and glucocorticoid content in microdissected brain regions: correlative aspects
url http://hdl.handle.net/20.500.12110/paper_00283835_v50_n6_p673_Magarinos
work_keys_str_mv AT magarinosam corticosteroidreceptorsandglucocorticoidcontentinmicrodissectedbrainregionscorrelativeaspects
AT ferrinim corticosteroidreceptorsandglucocorticoidcontentinmicrodissectedbrainregionscorrelativeaspects
AT denicolaaf corticosteroidreceptorsandglucocorticoidcontentinmicrodissectedbrainregionscorrelativeaspects
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