Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis
The effect of lipoxygenase inhibition, leukotriene agonists and antagonists, and 5-hydroxy-6, 8, l 1, 14-eicosate-traenoic acid (5-HF.TE) was examined in the rat pineal gland in organ culture. To study melatonin secretion pineal explants were incubated for 6 h in tissue culture medium 199 with the d...
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todo:paper_00283835_v46_n5_p412_Vacas2023-10-03T14:38:52Z Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis Vacas, M.I. Keller-Sarmiento, M.I. Etchegoyen, G.S. Pereyra, E.N. Gimeno, M.F. Cardinali, D.P. Leukotriene antagonists Leukotrienes Lipoxygenase inhibition Melatonin Pineal organ cultures 5 hydroxyicosatetraenoic acid leukotriene leukotriene c4 lipoxygenase melatonin noradrenalin nordihydroguaiaretic acid animal cell cell culture central nervous system drug antagonism drug comparison endocrine system in vitro study nonhuman pineal body rat Acetophenones Animals Arachidonate 5-Lipoxygenase Arachidonate Lipoxygenases Chromones Hydroxyeicosatetraenoic Acids Male Melatonin Nordihydroguaiaretic Acid Norepinephrine Organ Culture Techniques Pineal Gland Rats Rats, Inbred Strains SRS-A Tetrazoles The effect of lipoxygenase inhibition, leukotriene agonists and antagonists, and 5-hydroxy-6, 8, l 1, 14-eicosate-traenoic acid (5-HF.TE) was examined in the rat pineal gland in organ culture. To study melatonin secretion pineal explants were incubated for 6 h in tissue culture medium 199 with the different drugs. Melatonin concentration in the pineal gland and the medium was measured by RIA. Exposure of explants to norepinephrine (NE) brought about a 2-to 5-fold increase in both parameters, an effect that was reduced but not abolished, by the lipoxygenase inhibitor nordihydro-guaiaretic acid (NDGA: I0-5M). Lilly 171883 (10-5M) or FPL 55712 (10 5 M: Both antagonists of leukotrienes) reduced NE-induced melatonin production. Neither NDGA nor Lilly 171883 affected melatonin production in the absence of NE. Leukotrienes C4 and D4 increased melatonin release to the media at all concentrations tested (1-1, 000 n M) with a maximum effect at I nM(leukotriene C4) and 10 nM(leukotriene D4). Significantly higher tissue melatonin concentrations as compared to controls were observed after exposure of pineal explants to I and 100 nM of leukotriene C4, or 100 nM of leukotriene D4. Another 5-lipoxygenase metabolite, 5-HETE, increased pineal melatonin content at concentrations of 1, 10 and 100 nMwhereas only 1, 000 nM stimulated melatonin release. These results suggest that the 5-lipoxygenase pathway plays a significant role in NE-stimulated melatonin production by the rat pineal gland. © 1987 S. Karger AG, Basel. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00283835_v46_n5_p412_Vacas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Leukotriene antagonists Leukotrienes Lipoxygenase inhibition Melatonin Pineal organ cultures 5 hydroxyicosatetraenoic acid leukotriene leukotriene c4 lipoxygenase melatonin noradrenalin nordihydroguaiaretic acid animal cell cell culture central nervous system drug antagonism drug comparison endocrine system in vitro study nonhuman pineal body rat Acetophenones Animals Arachidonate 5-Lipoxygenase Arachidonate Lipoxygenases Chromones Hydroxyeicosatetraenoic Acids Male Melatonin Nordihydroguaiaretic Acid Norepinephrine Organ Culture Techniques Pineal Gland Rats Rats, Inbred Strains SRS-A Tetrazoles |
| spellingShingle |
Leukotriene antagonists Leukotrienes Lipoxygenase inhibition Melatonin Pineal organ cultures 5 hydroxyicosatetraenoic acid leukotriene leukotriene c4 lipoxygenase melatonin noradrenalin nordihydroguaiaretic acid animal cell cell culture central nervous system drug antagonism drug comparison endocrine system in vitro study nonhuman pineal body rat Acetophenones Animals Arachidonate 5-Lipoxygenase Arachidonate Lipoxygenases Chromones Hydroxyeicosatetraenoic Acids Male Melatonin Nordihydroguaiaretic Acid Norepinephrine Organ Culture Techniques Pineal Gland Rats Rats, Inbred Strains SRS-A Tetrazoles Vacas, M.I. Keller-Sarmiento, M.I. Etchegoyen, G.S. Pereyra, E.N. Gimeno, M.F. Cardinali, D.P. Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis |
| topic_facet |
Leukotriene antagonists Leukotrienes Lipoxygenase inhibition Melatonin Pineal organ cultures 5 hydroxyicosatetraenoic acid leukotriene leukotriene c4 lipoxygenase melatonin noradrenalin nordihydroguaiaretic acid animal cell cell culture central nervous system drug antagonism drug comparison endocrine system in vitro study nonhuman pineal body rat Acetophenones Animals Arachidonate 5-Lipoxygenase Arachidonate Lipoxygenases Chromones Hydroxyeicosatetraenoic Acids Male Melatonin Nordihydroguaiaretic Acid Norepinephrine Organ Culture Techniques Pineal Gland Rats Rats, Inbred Strains SRS-A Tetrazoles |
| description |
The effect of lipoxygenase inhibition, leukotriene agonists and antagonists, and 5-hydroxy-6, 8, l 1, 14-eicosate-traenoic acid (5-HF.TE) was examined in the rat pineal gland in organ culture. To study melatonin secretion pineal explants were incubated for 6 h in tissue culture medium 199 with the different drugs. Melatonin concentration in the pineal gland and the medium was measured by RIA. Exposure of explants to norepinephrine (NE) brought about a 2-to 5-fold increase in both parameters, an effect that was reduced but not abolished, by the lipoxygenase inhibitor nordihydro-guaiaretic acid (NDGA: I0-5M). Lilly 171883 (10-5M) or FPL 55712 (10 5 M: Both antagonists of leukotrienes) reduced NE-induced melatonin production. Neither NDGA nor Lilly 171883 affected melatonin production in the absence of NE. Leukotrienes C4 and D4 increased melatonin release to the media at all concentrations tested (1-1, 000 n M) with a maximum effect at I nM(leukotriene C4) and 10 nM(leukotriene D4). Significantly higher tissue melatonin concentrations as compared to controls were observed after exposure of pineal explants to I and 100 nM of leukotriene C4, or 100 nM of leukotriene D4. Another 5-lipoxygenase metabolite, 5-HETE, increased pineal melatonin content at concentrations of 1, 10 and 100 nMwhereas only 1, 000 nM stimulated melatonin release. These results suggest that the 5-lipoxygenase pathway plays a significant role in NE-stimulated melatonin production by the rat pineal gland. © 1987 S. Karger AG, Basel. |
| format |
JOUR |
| author |
Vacas, M.I. Keller-Sarmiento, M.I. Etchegoyen, G.S. Pereyra, E.N. Gimeno, M.F. Cardinali, D.P. |
| author_facet |
Vacas, M.I. Keller-Sarmiento, M.I. Etchegoyen, G.S. Pereyra, E.N. Gimeno, M.F. Cardinali, D.P. |
| author_sort |
Vacas, M.I. |
| title |
Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis |
| title_short |
Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis |
| title_full |
Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis |
| title_fullStr |
Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis |
| title_full_unstemmed |
Involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis |
| title_sort |
involvement of 5-lipoxygenase pathway in norepinephrine stimulation of rat pineal melatonin synthesis |
| url |
http://hdl.handle.net/20.500.12110/paper_00283835_v46_n5_p412_Vacas |
| work_keys_str_mv |
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