Kinetics, subcellular localization, and contribution to parasite virulence of a Trypanosoma cruzi hybrid type A heme peroxidase (TcAPx-CcP)

The Trypanosoma cruzi ascorbate peroxidase is, by sequence analysis, a hybrid type A member of class I heme peroxidases [TcAPx-cytochrome c peroxidase (CcP)], suggesting both ascorbate (Asc) and cytochrome c (Cc) peroxidase activity. Here, we show that the enzyme reacts fast with H2O2 (k = 2.9 × 107...

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Detalles Bibliográficos
Autores principales: Hugo, M., Martínez, A., Trujillo, M., Estrada, D., Mastrogiovanni, M., Linares, E., Augusto, O., Issoglio, F., Zeida, A., Estrín, D.A., Heijnen, H.F.G., Piacenza, L., Radi, R.
Formato: JOUR
Materias:
Heme peroxidase
Kinetics
Oxidants
Trypanosoma cruzi
Virulence
ascorbate peroxidase
cytochrome c peroxidase
heme
hydrogen peroxide
phenylalanine
cytochrome c
peroxidase
tryptophan
absorption spectroscopy
amastigote
animal cell
animal experiment
Article
cell kinetics
cell membrane
cellular distribution
controlled study
electron spin resonance
electron transport
enzyme activity
enzyme mechanism
enzyme substrate
host parasite interaction
mass spectrometry
mitochondrial membrane
molecular dynamics
mouse
nonhuman
parasite virulence
priority journal
protein expression
spin trapping
animal
Bagg albino mouse
C57BL mouse
Chagas disease
female
kinetics
male
metabolism
oxidation reduction reaction
parasite
parasitology
pathogenicity
physiology
procedures
site directed mutagenesis
virulence
Animals
Chagas Disease
Cytochrome c Group
Electron Spin Resonance Spectroscopy
Electron Transport
Female
Heme
Hydrogen Peroxide
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mutagenesis, Site-Directed
Oxidation-Reduction
Parasites
Peroxidase
Phenylalanine
Tryptophan
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00278424_v114_n8_pE1326_Hugo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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