Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates

Saccobolus saccoboloides, a coprophilous fungus, was grown in synthetic liquid media. Extracellular β-glucosidase, β-1,4 endoglucanase and β-1,4 exoglucanase induction and repression by carbohydrates were investigated. When endoglucanase and exoglucanase could not be measured, an alternative carboxy...

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Autores principales:	Magnelli, P., Forchiassin, F.
Formato:	JOUR
Materias:	Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00275514_v91_n2_p359_Magnelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00275514_v91_n2_p359_Magnelli
record_format	dspace
spelling	todo:paper_00275514_v91_n2_p359_Magnelli2023-10-03T14:37:43Z Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates Magnelli, P. Forchiassin, F. Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides Saccobolus saccoboloides, a coprophilous fungus, was grown in synthetic liquid media. Extracellular β-glucosidase, β-1,4 endoglucanase and β-1,4 exoglucanase induction and repression by carbohydrates were investigated. When endoglucanase and exoglucanase could not be measured, an alternative carboxymethylcellulose-clearing method was used. Crystalline cellulose was the best inducer, followed by lactose. The mixture of both inducers had an unexpected negative effect. Cellobiose or glucose repressed cellulase production in cellulose containing media. This repression was selective for some components of the cellulase complex, and was not observed using repressor concentrations below 3% w/v. Moreover, the degree of repression varied with the age of the cultures. Cellulase production by this fungus is inducible and subject to a complex repression by easily metabolized sugars. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00275514_v91_n2_p359_Magnelli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides
spellingShingle	Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides Magnelli, P. Forchiassin, F. Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates
topic_facet	Ascobolaceae Endoglucanase Exoglucanase β-glucosidase beta 1,4 endoglucanase beta 1,4 exoglucanase beta glucosidase carboxymethylcellulose cellobiose cellulase cellulose culture medium enzyme induction enzyme synthesis glucose lactose Saccobolus saccoboloides
description	Saccobolus saccoboloides, a coprophilous fungus, was grown in synthetic liquid media. Extracellular β-glucosidase, β-1,4 endoglucanase and β-1,4 exoglucanase induction and repression by carbohydrates were investigated. When endoglucanase and exoglucanase could not be measured, an alternative carboxymethylcellulose-clearing method was used. Crystalline cellulose was the best inducer, followed by lactose. The mixture of both inducers had an unexpected negative effect. Cellobiose or glucose repressed cellulase production in cellulose containing media. This repression was selective for some components of the cellulase complex, and was not observed using repressor concentrations below 3% w/v. Moreover, the degree of repression varied with the age of the cultures. Cellulase production by this fungus is inducible and subject to a complex repression by easily metabolized sugars.
format	JOUR
author	Magnelli, P. Forchiassin, F.
author_facet	Magnelli, P. Forchiassin, F.
author_sort	Magnelli, P.
title	Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates
title_short	Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates
title_full	Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates
title_fullStr	Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates
title_full_unstemmed	Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates
title_sort	regulation of the cellulase complex production by saccobolus saccoboloides: induction and repression by carbohydrates
url	http://hdl.handle.net/20.500.12110/paper_00275514_v91_n2_p359_Magnelli
work_keys_str_mv	AT magnellip regulationofthecellulasecomplexproductionbysaccobolussaccoboloidesinductionandrepressionbycarbohydrates AT forchiassinf regulationofthecellulasecomplexproductionbysaccobolussaccoboloidesinductionandrepressionbycarbohydrates
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Regulation of the cellulase complex production by Saccobolus saccoboloides: Induction and repression by carbohydrates

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