Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation

Mostrar todas las versiones(2)

Escherichia coli, cultured on minimal medium and deprived of its required amino-acids, was induced for lac genes transcription. After inducer removal and restoration of growth, β-galactosidase synthesis was measured. Two different kinetics of enzyme synthesis were observed depending on the starvatio...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: de Rivas, C.S., Mendez, B.S.
Formato: JOUR
Materias:
amino acid
beta galactosidase
lactose
messenger rna
escherichia coli
in vitro study
microorganism
operon
rna synthesis
starvation
theoretical study
Chromosomes, Bacterial
Comparative Study
Escherichia coli
F Factor
Genes
Kinetics
Lactose
RNA, Messenger
Translation, Genetic
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00268925_v156_n2_p229_deRivas
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00268925_v156_n2_p229_deRivas
record_format dspace
spelling todo:paper_00268925_v156_n2_p229_deRivas2023-10-03T14:37:08Z Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation de Rivas, C.S. Mendez, B.S. amino acid beta galactosidase lactose messenger rna escherichia coli in vitro study microorganism operon rna synthesis starvation theoretical study Chromosomes, Bacterial Comparative Study Escherichia coli F Factor Genes Kinetics Lactose RNA, Messenger Translation, Genetic Escherichia coli, cultured on minimal medium and deprived of its required amino-acids, was induced for lac genes transcription. After inducer removal and restoration of growth, β-galactosidase synthesis was measured. Two different kinetics of enzyme synthesis were observed depending on the starvation conditions employed during the induction period: 1. β-galactosidase synthesis was immediately obtained and a plateau was reached, in 20 min after restoration of growth, when cells had been induced during deprivation of amino-acids and carbon source. 2. β-galactosidase displayed an unusually long rate of synthesis and plateau was not reached before two doubling times, when cells had been induced during the deprivation of the sole amino-acids. The latter result points out a problem of messenger stability during those long translation kinetics and led us to study the behaviour of strains carrying lac genetic determinants on different replicative structures: chromosomic and plasmidic. In those two situations, induction of lac messenger RNA was obtained and ratify our previous observations. However, their translation kinetics suggest a DNA linkage of this induced messenger. © 1977 Springer-Verlag. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00268925_v156_n2_p229_deRivas
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic amino acid
beta galactosidase
lactose
messenger rna
escherichia coli
in vitro study
microorganism
operon
rna synthesis
starvation
theoretical study
Chromosomes, Bacterial
Comparative Study
Escherichia coli
F Factor
Genes
Kinetics
Lactose
RNA, Messenger
Translation, Genetic
spellingShingle amino acid
beta galactosidase
lactose
messenger rna
escherichia coli
in vitro study
microorganism
operon
rna synthesis
starvation
theoretical study
Chromosomes, Bacterial
Comparative Study
Escherichia coli
F Factor
Genes
Kinetics
Lactose
RNA, Messenger
Translation, Genetic
de Rivas, C.S.
Mendez, B.S.
Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation
topic_facet amino acid
beta galactosidase
lactose
messenger rna
escherichia coli
in vitro study
microorganism
operon
rna synthesis
starvation
theoretical study
Chromosomes, Bacterial
Comparative Study
Escherichia coli
F Factor
Genes
Kinetics
Lactose
RNA, Messenger
Translation, Genetic
description Escherichia coli, cultured on minimal medium and deprived of its required amino-acids, was induced for lac genes transcription. After inducer removal and restoration of growth, β-galactosidase synthesis was measured. Two different kinetics of enzyme synthesis were observed depending on the starvation conditions employed during the induction period: 1. β-galactosidase synthesis was immediately obtained and a plateau was reached, in 20 min after restoration of growth, when cells had been induced during deprivation of amino-acids and carbon source. 2. β-galactosidase displayed an unusually long rate of synthesis and plateau was not reached before two doubling times, when cells had been induced during the deprivation of the sole amino-acids. The latter result points out a problem of messenger stability during those long translation kinetics and led us to study the behaviour of strains carrying lac genetic determinants on different replicative structures: chromosomic and plasmidic. In those two situations, induction of lac messenger RNA was obtained and ratify our previous observations. However, their translation kinetics suggest a DNA linkage of this induced messenger. © 1977 Springer-Verlag.
format JOUR
author de Rivas, C.S.
Mendez, B.S.
author_facet de Rivas, C.S.
Mendez, B.S.
author_sort de Rivas, C.S.
title Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation
title_short Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation
title_full Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation
title_fullStr Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation
title_full_unstemmed Unusual stability and translation kinetics of an Escherichia coli lac messenger RNA synthetized during amino-acids deprivation
title_sort unusual stability and translation kinetics of an escherichia coli lac messenger rna synthetized during amino-acids deprivation
url http://hdl.handle.net/20.500.12110/paper_00268925_v156_n2_p229_deRivas
work_keys_str_mv AT derivascs unusualstabilityandtranslationkineticsofanescherichiacolilacmessengerrnasynthetizedduringaminoacidsdeprivation
AT mendezbs unusualstabilityandtranslationkineticsofanescherichiacolilacmessengerrnasynthetizedduringaminoacidsdeprivation
_version_ 1807322275191455744

Ejemplares similares