Catabolite translational effects on the lac messenger RNA of Escherichia coli K12

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The transcriptional and translational events occurring during the induction of the lac operon, were separated by blocking the translational step, either by aminoacid starvation or by addition of chloramphenicol. It was found that the carbon source used during the subsequent translation, affected the...

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Autores principales: de Rivas, C.S., Méndez, B.S.
Formato: JOUR
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00268925_v148_n1_p99_deRivas
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00268925_v148_n1_p99_deRivas2023-10-03T14:37:08Z Catabolite translational effects on the lac messenger RNA of Escherichia coli K12 de Rivas, C.S. Méndez, B.S. The transcriptional and translational events occurring during the induction of the lac operon, were separated by blocking the translational step, either by aminoacid starvation or by addition of chloramphenicol. It was found that the carbon source used during the subsequent translation, affected the rate of β-galactosidase synthesis. A decoordination effect on the production of enzymes of the lac system was also observed in high catabolite repression media, as well as in nitrogen limiting conditions. These findings suggested a similarity with the polarity phenomenon. In order to test this similarity, polarity suppressors of a Z- polar mutant were isolated. In one of these mutants, probably suA like, no carbon source effect was observed during the translational step. The induction kinetics in different media, after distinct pregrowth conditions, supported the idea that this mutant could be considered catabolite repression resistant only in certain restrictive conditions. © 1976 Springer-Verlag. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00268925_v148_n1_p99_deRivas
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description The transcriptional and translational events occurring during the induction of the lac operon, were separated by blocking the translational step, either by aminoacid starvation or by addition of chloramphenicol. It was found that the carbon source used during the subsequent translation, affected the rate of β-galactosidase synthesis. A decoordination effect on the production of enzymes of the lac system was also observed in high catabolite repression media, as well as in nitrogen limiting conditions. These findings suggested a similarity with the polarity phenomenon. In order to test this similarity, polarity suppressors of a Z- polar mutant were isolated. In one of these mutants, probably suA like, no carbon source effect was observed during the translational step. The induction kinetics in different media, after distinct pregrowth conditions, supported the idea that this mutant could be considered catabolite repression resistant only in certain restrictive conditions. © 1976 Springer-Verlag.
format JOUR
author de Rivas, C.S.
Méndez, B.S.
spellingShingle de Rivas, C.S.
Méndez, B.S.
Catabolite translational effects on the lac messenger RNA of Escherichia coli K12
author_facet de Rivas, C.S.
Méndez, B.S.
author_sort de Rivas, C.S.
title Catabolite translational effects on the lac messenger RNA of Escherichia coli K12
title_short Catabolite translational effects on the lac messenger RNA of Escherichia coli K12
title_full Catabolite translational effects on the lac messenger RNA of Escherichia coli K12
title_fullStr Catabolite translational effects on the lac messenger RNA of Escherichia coli K12
title_full_unstemmed Catabolite translational effects on the lac messenger RNA of Escherichia coli K12
title_sort catabolite translational effects on the lac messenger rna of escherichia coli k12
url http://hdl.handle.net/20.500.12110/paper_00268925_v148_n1_p99_deRivas
work_keys_str_mv AT derivascs catabolitetranslationaleffectsonthelacmessengerrnaofescherichiacolik12
AT mendezbs catabolitetranslationaleffectsonthelacmessengerrnaofescherichiacolik12
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