Role of different forms of fibronectin in in vitro bovine follicular development

This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor mRNA, play specific roles during follicular development. In particular, we analyzed the presence of the ED-I region, which is absent in the...

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Autores principales: Colman Lerner, A.A., Fischman, M.L., Lanuza, G., Cramer, P., Kornblihtt, A., Barañao, J.L.
Formato: JOUR
Materias:
fibronectin
animal
article
cattle
chemistry
female
growth, development and aging
ovary follicle
ovary follicle fluid
physiology
Animals
Cattle
Female
Fibronectins
Follicular Fluid
Ovarian Follicle
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p332_ColmanLerner
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00257680_v57_n3_p332_ColmanLerner
record_format dspace
spelling todo:paper_00257680_v57_n3_p332_ColmanLerner2023-10-03T14:36:31Z Role of different forms of fibronectin in in vitro bovine follicular development Colman Lerner, A.A. Fischman, M.L. Lanuza, G. Cramer, P. Kornblihtt, A. Barañao, J.L. fibronectin animal article cattle chemistry female growth, development and aging ovary follicle ovary follicle fluid physiology Animals Cattle Female Fibronectins Follicular Fluid Ovarian Follicle This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor mRNA, play specific roles during follicular development. In particular, we analyzed the presence of the ED-I region, which is absent in the plasma form. Analysis of FN levels in follicular fluids corresponding to different stages of development of bovine follicles revealed marked changes in the concentrations of ED-I+FN whereas total FN levels remained relatively constant. A negative correlation (P < 0.001) was detected between ED-I+FN and estradiol levels. This steroid was without effect on the alternative splicing of FN in primary cultures of bovine granulosa cells. However, cAMP produced a marked decrease in the incorporation of the ED-I region. In contrast, transforming growth factor beta (TGF-β) elicited both a stimulation on overall FN synthesis and an increase in the inclusion of ED-I. This effect was evident at the protein level (Western blots) and also in the mRNAs (Northern blots). A peptide corresponding to the ED-I region stimulated DNA synthesis in a bovine granulosa cell line (BGC-1) whereas the peptide corresponding to the flanking sequences was without effect. Data presented herein suggest a novel form of regulation by which changes in the primary structure of FN may mediate some of the effects of gonadotropin and intraovarian factors during follicular development. Fil:Colman Lerner, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lanuza, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cramer, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kornblihtt, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p332_ColmanLerner
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic fibronectin
animal
article
cattle
chemistry
female
growth, development and aging
ovary follicle
ovary follicle fluid
physiology
Animals
Cattle
Female
Fibronectins
Follicular Fluid
Ovarian Follicle
spellingShingle fibronectin
animal
article
cattle
chemistry
female
growth, development and aging
ovary follicle
ovary follicle fluid
physiology
Animals
Cattle
Female
Fibronectins
Follicular Fluid
Ovarian Follicle
Colman Lerner, A.A.
Fischman, M.L.
Lanuza, G.
Cramer, P.
Kornblihtt, A.
Barañao, J.L.
Role of different forms of fibronectin in in vitro bovine follicular development
topic_facet fibronectin
animal
article
cattle
chemistry
female
growth, development and aging
ovary follicle
ovary follicle fluid
physiology
Animals
Cattle
Female
Fibronectins
Follicular Fluid
Ovarian Follicle
description This study was aimed at testing the hypothesis that different forms of fibronectin (FN), produced as a consequence of the alternative splicing of the precursor mRNA, play specific roles during follicular development. In particular, we analyzed the presence of the ED-I region, which is absent in the plasma form. Analysis of FN levels in follicular fluids corresponding to different stages of development of bovine follicles revealed marked changes in the concentrations of ED-I+FN whereas total FN levels remained relatively constant. A negative correlation (P < 0.001) was detected between ED-I+FN and estradiol levels. This steroid was without effect on the alternative splicing of FN in primary cultures of bovine granulosa cells. However, cAMP produced a marked decrease in the incorporation of the ED-I region. In contrast, transforming growth factor beta (TGF-β) elicited both a stimulation on overall FN synthesis and an increase in the inclusion of ED-I. This effect was evident at the protein level (Western blots) and also in the mRNAs (Northern blots). A peptide corresponding to the ED-I region stimulated DNA synthesis in a bovine granulosa cell line (BGC-1) whereas the peptide corresponding to the flanking sequences was without effect. Data presented herein suggest a novel form of regulation by which changes in the primary structure of FN may mediate some of the effects of gonadotropin and intraovarian factors during follicular development.
format JOUR
author Colman Lerner, A.A.
Fischman, M.L.
Lanuza, G.
Cramer, P.
Kornblihtt, A.
Barañao, J.L.
author_facet Colman Lerner, A.A.
Fischman, M.L.
Lanuza, G.
Cramer, P.
Kornblihtt, A.
Barañao, J.L.
author_sort Colman Lerner, A.A.
title Role of different forms of fibronectin in in vitro bovine follicular development
title_short Role of different forms of fibronectin in in vitro bovine follicular development
title_full Role of different forms of fibronectin in in vitro bovine follicular development
title_fullStr Role of different forms of fibronectin in in vitro bovine follicular development
title_full_unstemmed Role of different forms of fibronectin in in vitro bovine follicular development
title_sort role of different forms of fibronectin in in vitro bovine follicular development
url http://hdl.handle.net/20.500.12110/paper_00257680_v57_n3_p332_ColmanLerner
work_keys_str_mv AT colmanlerneraa roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment
AT fischmanml roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment
AT lanuzag roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment
AT cramerp roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment
AT kornblihtta roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment
AT baranaojl roleofdifferentformsoffibronectinininvitrobovinefolliculardevelopment
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