Blood parameters variation in Calomys musculinus infected with Junin virus, strain XJCl3
The aim of this study was to analyze the alterations in homeostasis induced by Junin virus during acute and persistent infection of C. musculinus. Virus presence in brain, hematological response and glycemia levels were evaluated. Newborn C. musculinus inoculated with 4000 DL50 of Junin virus, strai...
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todo:paper_00257680_v50_n4_p335_Steyerthal2023-10-03T14:36:29Z Blood parameters variation in Calomys musculinus infected with Junin virus, strain XJCl3 Steyerthal, N.L. Lampuri, J.S. Coto, C.E. analysis of variance animal Arenavirus article Arvicolinae blood glucose blood level isolation and purification microbiology mouse serodiagnosis virus hemorrhagic fever Analysis of Variance Animals Arenaviruses, New World Arvicolinae Blood Glucose Hemorrhagic Fever, American Mice Neutralization Tests The aim of this study was to analyze the alterations in homeostasis induced by Junin virus during acute and persistent infection of C. musculinus. Virus presence in brain, hematological response and glycemia levels were evaluated. Newborn C. musculinus inoculated with 4000 DL50 of Junin virus, strain XJCl3 by intraperitoneal route developed a typical acute disease, with 50-70% mortality. Virus was isolated from brain starting day 6 post-infection (Fig. 1) and the peak titer (10(8) DL50/ml) was reached at 12 days post-infection. Neutralizing anti-Junin virus antibodies were detected from day 11 post-infection and all chronically infected animals developed persistent levels of neutralizing antibodies. In the acute stage of infection, 40% of the animals developed lymphopenia and neutrophilia (Fig. 2) while a slight variation was observed in the monocyte population. An important hypoglycemia was also seen in the acute infection (mean = 3.52 mmol/l) in comparison with control values (mean = 6.21 mmol/l), p less than 0.01 (Fig. 3). By contrast during the chronic stage of infection, neither hematological parameters (Table 2) varied between infected and control animals. Fil:Steyerthal, N.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lampuri, J.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00257680_v50_n4_p335_Steyerthal |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
analysis of variance animal Arenavirus article Arvicolinae blood glucose blood level isolation and purification microbiology mouse serodiagnosis virus hemorrhagic fever Analysis of Variance Animals Arenaviruses, New World Arvicolinae Blood Glucose Hemorrhagic Fever, American Mice Neutralization Tests |
| spellingShingle |
analysis of variance animal Arenavirus article Arvicolinae blood glucose blood level isolation and purification microbiology mouse serodiagnosis virus hemorrhagic fever Analysis of Variance Animals Arenaviruses, New World Arvicolinae Blood Glucose Hemorrhagic Fever, American Mice Neutralization Tests Steyerthal, N.L. Lampuri, J.S. Coto, C.E. Blood parameters variation in Calomys musculinus infected with Junin virus, strain XJCl3 |
| topic_facet |
analysis of variance animal Arenavirus article Arvicolinae blood glucose blood level isolation and purification microbiology mouse serodiagnosis virus hemorrhagic fever Analysis of Variance Animals Arenaviruses, New World Arvicolinae Blood Glucose Hemorrhagic Fever, American Mice Neutralization Tests |
| description |
The aim of this study was to analyze the alterations in homeostasis induced by Junin virus during acute and persistent infection of C. musculinus. Virus presence in brain, hematological response and glycemia levels were evaluated. Newborn C. musculinus inoculated with 4000 DL50 of Junin virus, strain XJCl3 by intraperitoneal route developed a typical acute disease, with 50-70% mortality. Virus was isolated from brain starting day 6 post-infection (Fig. 1) and the peak titer (10(8) DL50/ml) was reached at 12 days post-infection. Neutralizing anti-Junin virus antibodies were detected from day 11 post-infection and all chronically infected animals developed persistent levels of neutralizing antibodies. In the acute stage of infection, 40% of the animals developed lymphopenia and neutrophilia (Fig. 2) while a slight variation was observed in the monocyte population. An important hypoglycemia was also seen in the acute infection (mean = 3.52 mmol/l) in comparison with control values (mean = 6.21 mmol/l), p less than 0.01 (Fig. 3). By contrast during the chronic stage of infection, neither hematological parameters (Table 2) varied between infected and control animals. |
| format |
JOUR |
| author |
Steyerthal, N.L. Lampuri, J.S. Coto, C.E. |
| author_facet |
Steyerthal, N.L. Lampuri, J.S. Coto, C.E. |
| author_sort |
Steyerthal, N.L. |
| title |
Blood parameters variation in Calomys musculinus infected with Junin virus, strain XJCl3 |
| title_short |
Blood parameters variation in Calomys musculinus infected with Junin virus, strain XJCl3 |
| title_full |
Blood parameters variation in Calomys musculinus infected with Junin virus, strain XJCl3 |
| title_fullStr |
Blood parameters variation in Calomys musculinus infected with Junin virus, strain XJCl3 |
| title_full_unstemmed |
Blood parameters variation in Calomys musculinus infected with Junin virus, strain XJCl3 |
| title_sort |
blood parameters variation in calomys musculinus infected with junin virus, strain xjcl3 |
| url |
http://hdl.handle.net/20.500.12110/paper_00257680_v50_n4_p335_Steyerthal |
| work_keys_str_mv |
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| _version_ |
1807314467041574912 |