Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating...

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Autores principales: Saborowski, R., Thatje, S., Calcagno, J.A., Lovrich, G.A., Anger, K.
Formato: JOUR
Materias:
crab
enzyme activity
food availability
juvenile
larval development
low temperature
ontogeny
Antarctica
Chaceon affinis
Decapoda (Crustacea)
Limulus
Lithodes santolla
Proteidae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00253162_v149_n4_p865_Saborowski
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00253162_v149_n4_p865_Saborowski
record_format dspace
spelling todo:paper_00253162_v149_n4_p865_Saborowski2023-10-03T14:35:55Z Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla Saborowski, R. Thatje, S. Calcagno, J.A. Lovrich, G.A. Anger, K. crab enzyme activity food availability juvenile larval development low temperature ontogeny Antarctica Chaceon affinis Decapoda (Crustacea) Limulus Lithodes santolla Proteidae The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating the enzymatic ability to utilize endogeneous yolk reserves. SDS-PAGE showed a continuous decrease of proteins or proteids in the range of 59-81 kDa during ontogenetic development from the eggs through the zoeal stages to the first juvenile crab stage, CI. This reduction reflects the degradation of storage compounds during lecithotrophic larval development. Activities of the endopeptidases, trypsin and chymotrypsin, were low in eggs and larvae but increased significantly in the first juvenile crab stage. These enzymes typically facilitate the first steps of proteolysis in the extra-cellular spaces of the midgut gland and in the stomach. Their scarcity indicates that the larvae of L. santolla are physiologically not prepared to digest external food. This ability seems to appear first in the CI stage. Extracts of juvenile midgut glands and the gastric fluids of adults showed high activities of a variety of digestive enzymes including phosphatases, carbohydrases, as well as endo- and exopeptidases. High activities of digestive enzymes in adults may compensate for scarce food supply and rate-limiting low temperatures in the predominantly sub-Antarctic habitats of L. santolla. © Springer-Verlag 2006. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00253162_v149_n4_p865_Saborowski
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic crab
enzyme activity
food availability
juvenile
larval development
low temperature
ontogeny
Antarctica
Chaceon affinis
Decapoda (Crustacea)
Limulus
Lithodes santolla
Proteidae
spellingShingle crab
enzyme activity
food availability
juvenile
larval development
low temperature
ontogeny
Antarctica
Chaceon affinis
Decapoda (Crustacea)
Limulus
Lithodes santolla
Proteidae
Saborowski, R.
Thatje, S.
Calcagno, J.A.
Lovrich, G.A.
Anger, K.
Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla
topic_facet crab
enzyme activity
food availability
juvenile
larval development
low temperature
ontogeny
Antarctica
Chaceon affinis
Decapoda (Crustacea)
Limulus
Lithodes santolla
Proteidae
description The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating the enzymatic ability to utilize endogeneous yolk reserves. SDS-PAGE showed a continuous decrease of proteins or proteids in the range of 59-81 kDa during ontogenetic development from the eggs through the zoeal stages to the first juvenile crab stage, CI. This reduction reflects the degradation of storage compounds during lecithotrophic larval development. Activities of the endopeptidases, trypsin and chymotrypsin, were low in eggs and larvae but increased significantly in the first juvenile crab stage. These enzymes typically facilitate the first steps of proteolysis in the extra-cellular spaces of the midgut gland and in the stomach. Their scarcity indicates that the larvae of L. santolla are physiologically not prepared to digest external food. This ability seems to appear first in the CI stage. Extracts of juvenile midgut glands and the gastric fluids of adults showed high activities of a variety of digestive enzymes including phosphatases, carbohydrases, as well as endo- and exopeptidases. High activities of digestive enzymes in adults may compensate for scarce food supply and rate-limiting low temperatures in the predominantly sub-Antarctic habitats of L. santolla. © Springer-Verlag 2006.
format JOUR
author Saborowski, R.
Thatje, S.
Calcagno, J.A.
Lovrich, G.A.
Anger, K.
author_facet Saborowski, R.
Thatje, S.
Calcagno, J.A.
Lovrich, G.A.
Anger, K.
author_sort Saborowski, R.
title Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla
title_short Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla
title_full Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla
title_fullStr Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla
title_full_unstemmed Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla
title_sort digestive enzymes in the ontogenetic stages of the southern king crab, lithodes santolla
url http://hdl.handle.net/20.500.12110/paper_00253162_v149_n4_p865_Saborowski
work_keys_str_mv AT saborowskir digestiveenzymesintheontogeneticstagesofthesouthernkingcrablithodessantolla
AT thatjes digestiveenzymesintheontogeneticstagesofthesouthernkingcrablithodessantolla
AT calcagnoja digestiveenzymesintheontogeneticstagesofthesouthernkingcrablithodessantolla
AT lovrichga digestiveenzymesintheontogeneticstagesofthesouthernkingcrablithodessantolla
AT angerk digestiveenzymesintheontogeneticstagesofthesouthernkingcrablithodessantolla
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