Storage reserves and cellular water in mature seeds of Araucaria angustifolia

Seed tissues of Araucaria angustifolia (Bertol.) Kuntze were investigated using histochemistry, transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) analysis. Moisture content and water status in tissues were also evaluated. In the embryo, TEM studies revealed the presence of one...

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Autores principales: Panza, V., Láinez, V., Maroder, H., Prego, I., Maldonado, S.
Formato: JOUR
Materias:
Lipid bodies
Mineral nutrients
Mitochondria
Phytoferritin
Protein bodies
Protein storage vacuoles
Seed moisture content
Seed ultrastructure
Vacuole
storage
Araucaria
Araucaria angustifolia
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00244074_v140_n3_p273_Panza
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00244074_v140_n3_p273_Panza2023-10-03T14:35:02Z Storage reserves and cellular water in mature seeds of Araucaria angustifolia Panza, V. Láinez, V. Maroder, H. Prego, I. Maldonado, S. Lipid bodies Mineral nutrients Mitochondria Phytoferritin Protein bodies Protein storage vacuoles Seed moisture content Seed ultrastructure Vacuole storage Araucaria Araucaria angustifolia Seed tissues of Araucaria angustifolia (Bertol.) Kuntze were investigated using histochemistry, transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) analysis. Moisture content and water status in tissues were also evaluated. In the embryo, TEM studies revealed the presence of one to several central vacuoles and a peripheral layer of cytoplasm in cells from different tissues of the cotyledons and axis. In the cytoplasm, lipid bodies, starch grains, mitochondria and a nucleus are evident. In most tissues, vacuoles contain proteins, indicating that the storage proteins are highly hydrated. In cells of the root cap, proteins are stored in discrete protein bodies. Both protein storage vacuoles and discrete protein bodies have inclusions of crystal globoids. EDX analysis of globoids revealed the presence of P, K and Mg as the main constituents and traces of S, Ca and Fe. In the root and shoot meristems, deposits of phytoferritin are present in the stroma of proplastids. The gametophyte consists of cells characterized by relatively thin cell walls and one to several nuclei per cell. Protein and lipid bodies are present, although starch is the most conspicuous reserve. Immediately after shedding, moisture content is approximately 145% (dry weight) for the embryo and 95% (dry weight) for the gametophyte. Calorimetric studies reveal that axes and cotyledons have a very high content of freezable water, corresponding to types 5 and 4, i.e. dilute and concentrated (or capillary) solution, respectively. The results are discussed in relation to the behaviour of the species, which has been categorized as recalcitrant. © 2002 The Linnean Society of London. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00244074_v140_n3_p273_Panza
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Lipid bodies
Mineral nutrients
Mitochondria
Phytoferritin
Protein bodies
Protein storage vacuoles
Seed moisture content
Seed ultrastructure
Vacuole
storage
Araucaria
Araucaria angustifolia
spellingShingle Lipid bodies
Mineral nutrients
Mitochondria
Phytoferritin
Protein bodies
Protein storage vacuoles
Seed moisture content
Seed ultrastructure
Vacuole
storage
Araucaria
Araucaria angustifolia
Panza, V.
Láinez, V.
Maroder, H.
Prego, I.
Maldonado, S.
Storage reserves and cellular water in mature seeds of Araucaria angustifolia
topic_facet Lipid bodies
Mineral nutrients
Mitochondria
Phytoferritin
Protein bodies
Protein storage vacuoles
Seed moisture content
Seed ultrastructure
Vacuole
storage
Araucaria
Araucaria angustifolia
description Seed tissues of Araucaria angustifolia (Bertol.) Kuntze were investigated using histochemistry, transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) analysis. Moisture content and water status in tissues were also evaluated. In the embryo, TEM studies revealed the presence of one to several central vacuoles and a peripheral layer of cytoplasm in cells from different tissues of the cotyledons and axis. In the cytoplasm, lipid bodies, starch grains, mitochondria and a nucleus are evident. In most tissues, vacuoles contain proteins, indicating that the storage proteins are highly hydrated. In cells of the root cap, proteins are stored in discrete protein bodies. Both protein storage vacuoles and discrete protein bodies have inclusions of crystal globoids. EDX analysis of globoids revealed the presence of P, K and Mg as the main constituents and traces of S, Ca and Fe. In the root and shoot meristems, deposits of phytoferritin are present in the stroma of proplastids. The gametophyte consists of cells characterized by relatively thin cell walls and one to several nuclei per cell. Protein and lipid bodies are present, although starch is the most conspicuous reserve. Immediately after shedding, moisture content is approximately 145% (dry weight) for the embryo and 95% (dry weight) for the gametophyte. Calorimetric studies reveal that axes and cotyledons have a very high content of freezable water, corresponding to types 5 and 4, i.e. dilute and concentrated (or capillary) solution, respectively. The results are discussed in relation to the behaviour of the species, which has been categorized as recalcitrant. © 2002 The Linnean Society of London.
format JOUR
author Panza, V.
Láinez, V.
Maroder, H.
Prego, I.
Maldonado, S.
author_facet Panza, V.
Láinez, V.
Maroder, H.
Prego, I.
Maldonado, S.
author_sort Panza, V.
title Storage reserves and cellular water in mature seeds of Araucaria angustifolia
title_short Storage reserves and cellular water in mature seeds of Araucaria angustifolia
title_full Storage reserves and cellular water in mature seeds of Araucaria angustifolia
title_fullStr Storage reserves and cellular water in mature seeds of Araucaria angustifolia
title_full_unstemmed Storage reserves and cellular water in mature seeds of Araucaria angustifolia
title_sort storage reserves and cellular water in mature seeds of araucaria angustifolia
url http://hdl.handle.net/20.500.12110/paper_00244074_v140_n3_p273_Panza
work_keys_str_mv AT panzav storagereservesandcellularwaterinmatureseedsofaraucariaangustifolia
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AT maroderh storagereservesandcellularwaterinmatureseedsofaraucariaangustifolia
AT pregoi storagereservesandcellularwaterinmatureseedsofaraucariaangustifolia
AT maldonados storagereservesandcellularwaterinmatureseedsofaraucariaangustifolia
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