Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats

Recent work from our laboratory has demonstrated that the activation of GABA B adenohypophyseal receptors by baclofen inhibits pituitary hormone secretion under basal (PRL) or stimulated conditions (PRL and LH) in adult female rats, suggesting a hypophyseal site of action in addition to the central...

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Autores principales:	Rey-Roldan, E.B., Lux-Lantos, V.A.R., González-Iglesias, A.E., Becú-Villalobos, D., Libertun, C.
Formato:	JOUR
Materias:	Baclofen Infantile female rats Pituitary cells 4 aminobutyric acid B receptor 4 aminobutyric acid B receptor stimulating agent baclofen follitropin gonadorelin hypophysis hormone luteinizing hormone prolactin protirelin thyrotropin adenohypophysis animal cell article cell culture cell type concentration response controlled study development female hormone release hypophysis cell immaturity neuroendocrine system nonhuman rat Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00243205_v58_n13_p1059_ReyRoldan
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00243205_v58_n13_p1059_ReyRoldan2023-10-03T14:34:39Z Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats Rey-Roldan, E.B. Lux-Lantos, V.A.R. González-Iglesias, A.E. Becú-Villalobos, D. Libertun, C. Baclofen Infantile female rats Pituitary cells 4 aminobutyric acid B receptor 4 aminobutyric acid B receptor stimulating agent baclofen follitropin gonadorelin hypophysis hormone luteinizing hormone prolactin protirelin thyrotropin adenohypophysis animal cell article cell culture cell type concentration response controlled study development female hormone release hypophysis cell immaturity neuroendocrine system nonhuman rat Animalia Recent work from our laboratory has demonstrated that the activation of GABA B adenohypophyseal receptors by baclofen inhibits pituitary hormone secretion under basal (PRL) or stimulated conditions (PRL and LH) in adult female rats, suggesting a hypophyseal site of action in addition to the central site previously described. Since different patterns of hormone secretion are observed in infantile and adult rats, the purpose of the present study was to determine whether GABA B pituitary receptors were involved in endocrine responses at early stages of development. Pituitary cells of 12 day-old female rats were cultured in vitro and the effect of baclofen was determined in the presence or absence of stimulatory factors. Baclofen (1.10-9, 1.10-7 and 1.10-5 M) did not alter basal LH or FSH secretion but significantly inhibited the LHRH induced gonadotropins release after 30 or 60 minutes of incubation (after 60 minutes of incubation LH (%): control: 100 ± 5.6; BACL(1.10-7): 134.5 ± 25.8; LHRH(1.10-7): 596.7 ± 85.9; LHRH(1.10-7)-BACL(1.10-7): 374.7 ± 48.0; p < 0.01. FSH (%): control: 100 ± 6.5; BACL(1.10-7): 103.7 ± 6.5; LHRH(1.10-7): 283.9 ± 29.3; LHRH(1.10-7)-BACL(1.10-7): 183.0 ± 20.0; p < 0.01). Baclofen did not significantly modify either basal or TRH-stimulated PRL or TSH secretion. These results show that baclofen has direct effects on the secretion of adenohypophyseal cells of immature rats and that such effects are different from those observed in adult rats, and depend on the stage of development of the neuroendocrine controls of each cellular type. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00243205_v58_n13_p1059_ReyRoldan
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Baclofen Infantile female rats Pituitary cells 4 aminobutyric acid B receptor 4 aminobutyric acid B receptor stimulating agent baclofen follitropin gonadorelin hypophysis hormone luteinizing hormone prolactin protirelin thyrotropin adenohypophysis animal cell article cell culture cell type concentration response controlled study development female hormone release hypophysis cell immaturity neuroendocrine system nonhuman rat Animalia
spellingShingle	Baclofen Infantile female rats Pituitary cells 4 aminobutyric acid B receptor 4 aminobutyric acid B receptor stimulating agent baclofen follitropin gonadorelin hypophysis hormone luteinizing hormone prolactin protirelin thyrotropin adenohypophysis animal cell article cell culture cell type concentration response controlled study development female hormone release hypophysis cell immaturity neuroendocrine system nonhuman rat Animalia Rey-Roldan, E.B. Lux-Lantos, V.A.R. González-Iglesias, A.E. Becú-Villalobos, D. Libertun, C. Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats
topic_facet	Baclofen Infantile female rats Pituitary cells 4 aminobutyric acid B receptor 4 aminobutyric acid B receptor stimulating agent baclofen follitropin gonadorelin hypophysis hormone luteinizing hormone prolactin protirelin thyrotropin adenohypophysis animal cell article cell culture cell type concentration response controlled study development female hormone release hypophysis cell immaturity neuroendocrine system nonhuman rat Animalia
description	Recent work from our laboratory has demonstrated that the activation of GABA B adenohypophyseal receptors by baclofen inhibits pituitary hormone secretion under basal (PRL) or stimulated conditions (PRL and LH) in adult female rats, suggesting a hypophyseal site of action in addition to the central site previously described. Since different patterns of hormone secretion are observed in infantile and adult rats, the purpose of the present study was to determine whether GABA B pituitary receptors were involved in endocrine responses at early stages of development. Pituitary cells of 12 day-old female rats were cultured in vitro and the effect of baclofen was determined in the presence or absence of stimulatory factors. Baclofen (1.10-9, 1.10-7 and 1.10-5 M) did not alter basal LH or FSH secretion but significantly inhibited the LHRH induced gonadotropins release after 30 or 60 minutes of incubation (after 60 minutes of incubation LH (%): control: 100 ± 5.6; BACL(1.10-7): 134.5 ± 25.8; LHRH(1.10-7): 596.7 ± 85.9; LHRH(1.10-7)-BACL(1.10-7): 374.7 ± 48.0; p < 0.01. FSH (%): control: 100 ± 6.5; BACL(1.10-7): 103.7 ± 6.5; LHRH(1.10-7): 283.9 ± 29.3; LHRH(1.10-7)-BACL(1.10-7): 183.0 ± 20.0; p < 0.01). Baclofen did not significantly modify either basal or TRH-stimulated PRL or TSH secretion. These results show that baclofen has direct effects on the secretion of adenohypophyseal cells of immature rats and that such effects are different from those observed in adult rats, and depend on the stage of development of the neuroendocrine controls of each cellular type.
format	JOUR
author	Rey-Roldan, E.B. Lux-Lantos, V.A.R. González-Iglesias, A.E. Becú-Villalobos, D. Libertun, C.
author_facet	Rey-Roldan, E.B. Lux-Lantos, V.A.R. González-Iglesias, A.E. Becú-Villalobos, D. Libertun, C.
author_sort	Rey-Roldan, E.B.
title	Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats
title_short	Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats
title_full	Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats
title_fullStr	Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats
title_full_unstemmed	Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats
title_sort	baclofen, a gamma-aminobutyric acid b agonist, modifies hormonal secretion in pituitary cells from infantile female rats
url	http://hdl.handle.net/20.500.12110/paper_00243205_v58_n13_p1059_ReyRoldan
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Baclofen, a gamma-aminobutyric acid B agonist, modifies hormonal secretion in pituitary cells from infantile female rats

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