Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia
Muscarinic receptors were assessed by [3H]-quinuclidinyl benzilate (QNB) binding in 900 xg supernatants of bovine superior cervical ganglia (SCG). At 30°C half maximal binding was reached within 3 min and equilibrium within 30 min. Scatchard analysis revealed a single population of binding sites wit...
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todo:paper_00243205_v32_n9_p965_Gejman2023-10-03T14:34:34Z Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia Gejman, P.V. Cardinali, D.P. angiotensin bradykinin estradiol follitropin gonadorelin growth hormone liothyronine luteinizing hormone muscarinic receptor prolactin protirelin radioisotope testosterone thyrotropin thyroxine animal cell autonomic nervous system cattle drug efficacy drug receptor binding nonhuman pharmacokinetics quinuclidine derivative h 3 quinuclidinyl benzilate h 3 rat superior cervical ganglion Animal Cattle Female Ganglia, Sympathetic Hormones Luteinizing Hormone Male Prolactin Protirelin Quinuclidinyl Benzilate Rats Rats, Inbred Strains Receptors, Cholinergic Receptors, Muscarinic Support, Non-U.S. Gov't Testosterone Muscarinic receptors were assessed by [3H]-quinuclidinyl benzilate (QNB) binding in 900 xg supernatants of bovine superior cervical ganglia (SCG). At 30°C half maximal binding was reached within 3 min and equilibrium within 30 min. Scatchard analysis revealed a single population of binding sites with dissociation constant (Kd)= 0.15 ± 0.01 nM and site concentration (Bmax)= 101 ± 4 fmoles/mg prot. Binding was specific for muscarinic drugs. Incubation of bovine SCG with different hormones (10-7M) indicated that LH, TRH and testosterone depressed significantly Bmax, and that prolactin decreased both Kd and Bmax of [3H]-QNB binding. Several other hormones tested (TSH, GH, FSH, LHRH, angiotensin II, bradykinin, melatonin, estradiol, thyroxine and triiodothyronine) did not affect QNB binding. Hormone effects were not due to a direct interference with radioligand binding to membrane. The injection of LH to orchidectomized rats depressed Bmax of SCG QNB binding without changing the Kd. These results suggest that muscarinic cholinergic neurotransmission in SCG may be affected by hormones. © 1983. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00243205_v32_n9_p965_Gejman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
angiotensin bradykinin estradiol follitropin gonadorelin growth hormone liothyronine luteinizing hormone muscarinic receptor prolactin protirelin radioisotope testosterone thyrotropin thyroxine animal cell autonomic nervous system cattle drug efficacy drug receptor binding nonhuman pharmacokinetics quinuclidine derivative h 3 quinuclidinyl benzilate h 3 rat superior cervical ganglion Animal Cattle Female Ganglia, Sympathetic Hormones Luteinizing Hormone Male Prolactin Protirelin Quinuclidinyl Benzilate Rats Rats, Inbred Strains Receptors, Cholinergic Receptors, Muscarinic Support, Non-U.S. Gov't Testosterone |
| spellingShingle |
angiotensin bradykinin estradiol follitropin gonadorelin growth hormone liothyronine luteinizing hormone muscarinic receptor prolactin protirelin radioisotope testosterone thyrotropin thyroxine animal cell autonomic nervous system cattle drug efficacy drug receptor binding nonhuman pharmacokinetics quinuclidine derivative h 3 quinuclidinyl benzilate h 3 rat superior cervical ganglion Animal Cattle Female Ganglia, Sympathetic Hormones Luteinizing Hormone Male Prolactin Protirelin Quinuclidinyl Benzilate Rats Rats, Inbred Strains Receptors, Cholinergic Receptors, Muscarinic Support, Non-U.S. Gov't Testosterone Gejman, P.V. Cardinali, D.P. Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia |
| topic_facet |
angiotensin bradykinin estradiol follitropin gonadorelin growth hormone liothyronine luteinizing hormone muscarinic receptor prolactin protirelin radioisotope testosterone thyrotropin thyroxine animal cell autonomic nervous system cattle drug efficacy drug receptor binding nonhuman pharmacokinetics quinuclidine derivative h 3 quinuclidinyl benzilate h 3 rat superior cervical ganglion Animal Cattle Female Ganglia, Sympathetic Hormones Luteinizing Hormone Male Prolactin Protirelin Quinuclidinyl Benzilate Rats Rats, Inbred Strains Receptors, Cholinergic Receptors, Muscarinic Support, Non-U.S. Gov't Testosterone |
| description |
Muscarinic receptors were assessed by [3H]-quinuclidinyl benzilate (QNB) binding in 900 xg supernatants of bovine superior cervical ganglia (SCG). At 30°C half maximal binding was reached within 3 min and equilibrium within 30 min. Scatchard analysis revealed a single population of binding sites with dissociation constant (Kd)= 0.15 ± 0.01 nM and site concentration (Bmax)= 101 ± 4 fmoles/mg prot. Binding was specific for muscarinic drugs. Incubation of bovine SCG with different hormones (10-7M) indicated that LH, TRH and testosterone depressed significantly Bmax, and that prolactin decreased both Kd and Bmax of [3H]-QNB binding. Several other hormones tested (TSH, GH, FSH, LHRH, angiotensin II, bradykinin, melatonin, estradiol, thyroxine and triiodothyronine) did not affect QNB binding. Hormone effects were not due to a direct interference with radioligand binding to membrane. The injection of LH to orchidectomized rats depressed Bmax of SCG QNB binding without changing the Kd. These results suggest that muscarinic cholinergic neurotransmission in SCG may be affected by hormones. © 1983. |
| format |
JOUR |
| author |
Gejman, P.V. Cardinali, D.P. |
| author_facet |
Gejman, P.V. Cardinali, D.P. |
| author_sort |
Gejman, P.V. |
| title |
Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia |
| title_short |
Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia |
| title_full |
Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia |
| title_fullStr |
Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia |
| title_full_unstemmed |
Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia |
| title_sort |
hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia |
| url |
http://hdl.handle.net/20.500.12110/paper_00243205_v32_n9_p965_Gejman |
| work_keys_str_mv |
AT gejmanpv hormoneeffectsonmuscariniccholinergicbindinginbovineandratsympatheticsuperiorcervicalganglia AT cardinalidp hormoneeffectsonmuscariniccholinergicbindinginbovineandratsympatheticsuperiorcervicalganglia |
| _version_ |
1782028516269752320 |