Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry

Flow cytometry (FCM) is a powerful tool for analyzing physiological characteristics of microorganisms on a single-cell basis and identifying heterogeneities within population. This work analyzed the UV-C induced damage on Escherichia coli ATCC 11229; Listeria innocua ATCC 33090 and Saccharomyces cer...

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Autores principales: Schenk, M., Raffellini, S., Guerrero, S., Blanco, G.A., Alzamora, S.M.
Formato: JOUR
Materias:
E. coli
Flow cytometry
Inactivation
L. innocua
S. cerevisiae
UV-C light
S.cerevisiae
Diseases
Listeria
Meteorological instruments
Microorganisms
Ultraviolet radiation
Yeast
Escherichia coli
Listeria innocua
Saccharomyces cerevisiae
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00236438_v44_n1_p191_Schenk
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00236438_v44_n1_p191_Schenk
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spelling todo:paper_00236438_v44_n1_p191_Schenk2023-10-03T14:34:09Z Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry Schenk, M. Raffellini, S. Guerrero, S. Blanco, G.A. Alzamora, S.M. E. coli Flow cytometry Inactivation L. innocua S. cerevisiae UV-C light E. coli Inactivation L. innocua S.cerevisiae UV-C light Diseases Flow cytometry Listeria Meteorological instruments Microorganisms Ultraviolet radiation Yeast Escherichia coli Escherichia coli Listeria innocua Saccharomyces cerevisiae Flow cytometry (FCM) is a powerful tool for analyzing physiological characteristics of microorganisms on a single-cell basis and identifying heterogeneities within population. This work analyzed the UV-C induced damage on Escherichia coli ATCC 11229; Listeria innocua ATCC 33090 and Saccharomyces cerevisiae KE162 cells by applying flow cytometry technique. The UV-C doses, obtained by altering the exposure time and measured by the iodide-iodate chemical actinometer, ranged between 0 and 5 kJ/m2. E. coli; L. innocua and S. cerevisiae populations were quantified by plate count technique. For flow cytometry studies, cells were labeled with fluorescein diacetate (FDA) for detecting membrane integrity and esterase activity, and with propidium iodide (PI) for monitoring membrane integrity. The results showed that mechanisms of cellular damage differed according to time of exposure to ultraviolet radiation and the organism tested. E. coli and S. cerevisiae sub-populations with PI increased within the first minutes of UV-C treatment, without much change afterwards. On the contrary, FCM was used to detect the inactivation of those L. innocua sub-populations of viable microorganisms (maintaining metabolic activity) which were non-culturable due to membrane rupture and thus not detectable by viable plate count technique. © 2010 Elsevier Ltd. Fil:Schenk, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Raffellini, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerrero, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Alzamora, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00236438_v44_n1_p191_Schenk
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic E. coli
Flow cytometry
Inactivation
L. innocua
S. cerevisiae
UV-C light
E. coli
Inactivation
L. innocua
S.cerevisiae
UV-C light
Diseases
Flow cytometry
Listeria
Meteorological instruments
Microorganisms
Ultraviolet radiation
Yeast
Escherichia coli
Escherichia coli
Listeria innocua
Saccharomyces cerevisiae
spellingShingle E. coli
Flow cytometry
Inactivation
L. innocua
S. cerevisiae
UV-C light
E. coli
Inactivation
L. innocua
S.cerevisiae
UV-C light
Diseases
Flow cytometry
Listeria
Meteorological instruments
Microorganisms
Ultraviolet radiation
Yeast
Escherichia coli
Escherichia coli
Listeria innocua
Saccharomyces cerevisiae
Schenk, M.
Raffellini, S.
Guerrero, S.
Blanco, G.A.
Alzamora, S.M.
Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
topic_facet E. coli
Flow cytometry
Inactivation
L. innocua
S. cerevisiae
UV-C light
E. coli
Inactivation
L. innocua
S.cerevisiae
UV-C light
Diseases
Flow cytometry
Listeria
Meteorological instruments
Microorganisms
Ultraviolet radiation
Yeast
Escherichia coli
Escherichia coli
Listeria innocua
Saccharomyces cerevisiae
description Flow cytometry (FCM) is a powerful tool for analyzing physiological characteristics of microorganisms on a single-cell basis and identifying heterogeneities within population. This work analyzed the UV-C induced damage on Escherichia coli ATCC 11229; Listeria innocua ATCC 33090 and Saccharomyces cerevisiae KE162 cells by applying flow cytometry technique. The UV-C doses, obtained by altering the exposure time and measured by the iodide-iodate chemical actinometer, ranged between 0 and 5 kJ/m2. E. coli; L. innocua and S. cerevisiae populations were quantified by plate count technique. For flow cytometry studies, cells were labeled with fluorescein diacetate (FDA) for detecting membrane integrity and esterase activity, and with propidium iodide (PI) for monitoring membrane integrity. The results showed that mechanisms of cellular damage differed according to time of exposure to ultraviolet radiation and the organism tested. E. coli and S. cerevisiae sub-populations with PI increased within the first minutes of UV-C treatment, without much change afterwards. On the contrary, FCM was used to detect the inactivation of those L. innocua sub-populations of viable microorganisms (maintaining metabolic activity) which were non-culturable due to membrane rupture and thus not detectable by viable plate count technique. © 2010 Elsevier Ltd.
format JOUR
author Schenk, M.
Raffellini, S.
Guerrero, S.
Blanco, G.A.
Alzamora, S.M.
author_facet Schenk, M.
Raffellini, S.
Guerrero, S.
Blanco, G.A.
Alzamora, S.M.
author_sort Schenk, M.
title Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_short Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_full Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_fullStr Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_full_unstemmed Inactivation of Escherichia coli, Listeria innocua and Saccharomyces cerevisiae by UV-C light: Study of cell injury by flow cytometry
title_sort inactivation of escherichia coli, listeria innocua and saccharomyces cerevisiae by uv-c light: study of cell injury by flow cytometry
url http://hdl.handle.net/20.500.12110/paper_00236438_v44_n1_p191_Schenk
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AT raffellinis inactivationofescherichiacolilisteriainnocuaandsaccharomycescerevisiaebyuvclightstudyofcellinjurybyflowcytometry
AT guerreros inactivationofescherichiacolilisteriainnocuaandsaccharomycescerevisiaebyuvclightstudyofcellinjurybyflowcytometry
AT blancoga inactivationofescherichiacolilisteriainnocuaandsaccharomycescerevisiaebyuvclightstudyofcellinjurybyflowcytometry
AT alzamorasm inactivationofescherichiacolilisteriainnocuaandsaccharomycescerevisiaebyuvclightstudyofcellinjurybyflowcytometry
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