The antioxidant enzymatic blood profile in Alzheimer's and vascular diseases. Their association and a possible assay to differentiate demented subjects and controls
A study of several elements of the antioxidative system: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione system (GLU), chemiluminescence (CHE), and antioxidant capacity (AOX), was conducted in 20 demented probable Alzheimer's (DAT), and 15 vascular demented (VD) patients, 19 contr...
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todo:paper_0022510X_v141_n1-2_p69_Famulari2023-10-03T14:33:25Z The antioxidant enzymatic blood profile in Alzheimer's and vascular diseases. Their association and a possible assay to differentiate demented subjects and controls Famulari, A.L. Marschoff, E.R. Llesuy, S.F. Kohan, S. Serra, J.A. Dominguez, R.O. Repetto, M. Reides, C. De Lustig, E.S. Antioxidant enzyme Catalse Cu-Zn superoxide dismutase Dementia of Alzheimer's type Glutathione system Oxygen free radical Periphral marker Vascular dementia catalase copper zinc superoxide dismutase free radical glutathione hydrogen peroxide adult aged Alzheimer disease antioxidant activity article chemoluminescence controlled study female human major clinical study male multiinfarct dementia priority journal A study of several elements of the antioxidative system: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione system (GLU), chemiluminescence (CHE), and antioxidant capacity (AOX), was conducted in 20 demented probable Alzheimer's (DAT), and 15 vascular demented (VD) patients, 19 control (C) subjects, and 11 relatives (F) of one DAT patient. A significant association was found between the variables of the antioxidant system, measured in blood samples, and the neurological pathologies VD and DAT: Kruskal-WalIis test; p = 0.0006 (p = 0.014 when the analysis did not include SOD). This demonstrated that VD and DAT diseases are accompanied by oxidative disorders, The VD and DAT diseases are differentially distinguishable by changes in blood profiles. A graphical method for classification, the Principal Components Analysis (PCA), distinguished between demented and non-demented subjects on the basis of their laboratory variables. A numerical method, Discriminant Functions (DF), constructed to separate the clinical groups on the basis of the same variables, obtained relatively high percentages of success: 92% of demented were detected against healthy subjects; of the latter 82% have been correctly identified as non-demented. Discrimination between VD and DAT patients was achieved for 100% of VD and 86% of DAT patients, DF were similarly successful in detecting the healthy condition of DAT relatives. Possible different mechanisms involved in H2O2 elimination in DAT and VD patients are proposed, where CAT is the responsible enzyme of this reaction in DAT patients, while in VD this function would be achieved mainly through the action of GLU. It seems that SOD levels are stable, at least, within one year. Variations appear to be linked with clinical changes. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0022510X_v141_n1-2_p69_Famulari |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antioxidant enzyme Catalse Cu-Zn superoxide dismutase Dementia of Alzheimer's type Glutathione system Oxygen free radical Periphral marker Vascular dementia catalase copper zinc superoxide dismutase free radical glutathione hydrogen peroxide adult aged Alzheimer disease antioxidant activity article chemoluminescence controlled study female human major clinical study male multiinfarct dementia priority journal |
| spellingShingle |
Antioxidant enzyme Catalse Cu-Zn superoxide dismutase Dementia of Alzheimer's type Glutathione system Oxygen free radical Periphral marker Vascular dementia catalase copper zinc superoxide dismutase free radical glutathione hydrogen peroxide adult aged Alzheimer disease antioxidant activity article chemoluminescence controlled study female human major clinical study male multiinfarct dementia priority journal Famulari, A.L. Marschoff, E.R. Llesuy, S.F. Kohan, S. Serra, J.A. Dominguez, R.O. Repetto, M. Reides, C. De Lustig, E.S. The antioxidant enzymatic blood profile in Alzheimer's and vascular diseases. Their association and a possible assay to differentiate demented subjects and controls |
| topic_facet |
Antioxidant enzyme Catalse Cu-Zn superoxide dismutase Dementia of Alzheimer's type Glutathione system Oxygen free radical Periphral marker Vascular dementia catalase copper zinc superoxide dismutase free radical glutathione hydrogen peroxide adult aged Alzheimer disease antioxidant activity article chemoluminescence controlled study female human major clinical study male multiinfarct dementia priority journal |
| description |
A study of several elements of the antioxidative system: Cu-Zn superoxide dismutase (SOD), catalase (CAT), glutathione system (GLU), chemiluminescence (CHE), and antioxidant capacity (AOX), was conducted in 20 demented probable Alzheimer's (DAT), and 15 vascular demented (VD) patients, 19 control (C) subjects, and 11 relatives (F) of one DAT patient. A significant association was found between the variables of the antioxidant system, measured in blood samples, and the neurological pathologies VD and DAT: Kruskal-WalIis test; p = 0.0006 (p = 0.014 when the analysis did not include SOD). This demonstrated that VD and DAT diseases are accompanied by oxidative disorders, The VD and DAT diseases are differentially distinguishable by changes in blood profiles. A graphical method for classification, the Principal Components Analysis (PCA), distinguished between demented and non-demented subjects on the basis of their laboratory variables. A numerical method, Discriminant Functions (DF), constructed to separate the clinical groups on the basis of the same variables, obtained relatively high percentages of success: 92% of demented were detected against healthy subjects; of the latter 82% have been correctly identified as non-demented. Discrimination between VD and DAT patients was achieved for 100% of VD and 86% of DAT patients, DF were similarly successful in detecting the healthy condition of DAT relatives. Possible different mechanisms involved in H2O2 elimination in DAT and VD patients are proposed, where CAT is the responsible enzyme of this reaction in DAT patients, while in VD this function would be achieved mainly through the action of GLU. It seems that SOD levels are stable, at least, within one year. Variations appear to be linked with clinical changes. |
| format |
JOUR |
| author |
Famulari, A.L. Marschoff, E.R. Llesuy, S.F. Kohan, S. Serra, J.A. Dominguez, R.O. Repetto, M. Reides, C. De Lustig, E.S. |
| author_facet |
Famulari, A.L. Marschoff, E.R. Llesuy, S.F. Kohan, S. Serra, J.A. Dominguez, R.O. Repetto, M. Reides, C. De Lustig, E.S. |
| author_sort |
Famulari, A.L. |
| title |
The antioxidant enzymatic blood profile in Alzheimer's and vascular diseases. Their association and a possible assay to differentiate demented subjects and controls |
| title_short |
The antioxidant enzymatic blood profile in Alzheimer's and vascular diseases. Their association and a possible assay to differentiate demented subjects and controls |
| title_full |
The antioxidant enzymatic blood profile in Alzheimer's and vascular diseases. Their association and a possible assay to differentiate demented subjects and controls |
| title_fullStr |
The antioxidant enzymatic blood profile in Alzheimer's and vascular diseases. Their association and a possible assay to differentiate demented subjects and controls |
| title_full_unstemmed |
The antioxidant enzymatic blood profile in Alzheimer's and vascular diseases. Their association and a possible assay to differentiate demented subjects and controls |
| title_sort |
antioxidant enzymatic blood profile in alzheimer's and vascular diseases. their association and a possible assay to differentiate demented subjects and controls |
| url |
http://hdl.handle.net/20.500.12110/paper_0022510X_v141_n1-2_p69_Famulari |
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