Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone

Metabolic properties and subcellular localization of the biosynthesis of SM, a saponifiable 18-OH-B (18-Hydroxycorticosterone) derivative, were investigated. Homogenates biosynthesized SM at a nearly constant rate of 463 pmol/50 mg tissue during 30 min. This biosynthesis was more efficient at pH 7.4...

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Autores principales:	Cozza, E.N., Ceballos, N.R., del Carmen Vila, M., Lantos, C.P.
Formato:	JOUR
Materias:	18 hydroxycorticosterone corticosterone drug derivative adrenal gland animal article cell fractionation cell nucleus cytosol kinetics metabolism microsome mitochondrion rat rat strain solubility 18-Hydroxycorticosterone Adrenal Glands Animal Cell Nucleus Corticosterone Cytosol Kinetics Microsomes Mitochondria Rats Rats, Inbred Strains Solubility Subcellular Fractions Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00224731_v28_n5_p543_Cozza
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00224731_v28_n5_p543_Cozza
record_format	dspace
spelling	todo:paper_00224731_v28_n5_p543_Cozza2023-10-03T14:33:07Z Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone Cozza, E.N. Ceballos, N.R. del Carmen Vila, M. Lantos, C.P. 18 hydroxycorticosterone corticosterone drug derivative adrenal gland animal article cell fractionation cell nucleus cytosol kinetics metabolism microsome mitochondrion rat rat strain solubility 18-Hydroxycorticosterone Adrenal Glands Animal Cell Nucleus Corticosterone Cytosol Kinetics Microsomes Mitochondria Rats Rats, Inbred Strains Solubility Subcellular Fractions Support, Non-U.S. Gov't Metabolic properties and subcellular localization of the biosynthesis of SM, a saponifiable 18-OH-B (18-Hydroxycorticosterone) derivative, were investigated. Homogenates biosynthesized SM at a nearly constant rate of 463 pmol/50 mg tissue during 30 min. This biosynthesis was more efficient at pH 7.4 than at pH 4.8. Not only 18-OH-B but also its less polar anhydride 18-DAL (18-Deoxyaldosterone) were good precursors. SM was reverted to these precursors both enzymatically and spontaneously, 4.8 being a more suitable pH for this reversion than 7.4. Trapping experiments demonstrated a sequence comprising, in this order, the following echelons: SM, 18-OH-B, 18-DAL, Aldosterone. The first two steps are reversible and the last two ones depend on proton concentrations. It is postulated that SM could be on a dead-end to which 18-OH-B could be deviated if Aldosterone biosynthesis became temporarily unnecessary. Also, that 18-OH-B may convert to either 18-DAL or SM for selective membrane transports, according to homeostatic requirements. © 1987. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ceballos, N.R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Carmen Vila, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224731_v28_n5_p543_Cozza
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	18 hydroxycorticosterone corticosterone drug derivative adrenal gland animal article cell fractionation cell nucleus cytosol kinetics metabolism microsome mitochondrion rat rat strain solubility 18-Hydroxycorticosterone Adrenal Glands Animal Cell Nucleus Corticosterone Cytosol Kinetics Microsomes Mitochondria Rats Rats, Inbred Strains Solubility Subcellular Fractions Support, Non-U.S. Gov't
spellingShingle	18 hydroxycorticosterone corticosterone drug derivative adrenal gland animal article cell fractionation cell nucleus cytosol kinetics metabolism microsome mitochondrion rat rat strain solubility 18-Hydroxycorticosterone Adrenal Glands Animal Cell Nucleus Corticosterone Cytosol Kinetics Microsomes Mitochondria Rats Rats, Inbred Strains Solubility Subcellular Fractions Support, Non-U.S. Gov't Cozza, E.N. Ceballos, N.R. del Carmen Vila, M. Lantos, C.P. Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
topic_facet	18 hydroxycorticosterone corticosterone drug derivative adrenal gland animal article cell fractionation cell nucleus cytosol kinetics metabolism microsome mitochondrion rat rat strain solubility 18-Hydroxycorticosterone Adrenal Glands Animal Cell Nucleus Corticosterone Cytosol Kinetics Microsomes Mitochondria Rats Rats, Inbred Strains Solubility Subcellular Fractions Support, Non-U.S. Gov't
description	Metabolic properties and subcellular localization of the biosynthesis of SM, a saponifiable 18-OH-B (18-Hydroxycorticosterone) derivative, were investigated. Homogenates biosynthesized SM at a nearly constant rate of 463 pmol/50 mg tissue during 30 min. This biosynthesis was more efficient at pH 7.4 than at pH 4.8. Not only 18-OH-B but also its less polar anhydride 18-DAL (18-Deoxyaldosterone) were good precursors. SM was reverted to these precursors both enzymatically and spontaneously, 4.8 being a more suitable pH for this reversion than 7.4. Trapping experiments demonstrated a sequence comprising, in this order, the following echelons: SM, 18-OH-B, 18-DAL, Aldosterone. The first two steps are reversible and the last two ones depend on proton concentrations. It is postulated that SM could be on a dead-end to which 18-OH-B could be deviated if Aldosterone biosynthesis became temporarily unnecessary. Also, that 18-OH-B may convert to either 18-DAL or SM for selective membrane transports, according to homeostatic requirements. © 1987.
format	JOUR
author	Cozza, E.N. Ceballos, N.R. del Carmen Vila, M. Lantos, C.P.
author_facet	Cozza, E.N. Ceballos, N.R. del Carmen Vila, M. Lantos, C.P.
author_sort	Cozza, E.N.
title	Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_short	Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_full	Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_fullStr	Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_full_unstemmed	Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
title_sort	convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone
url	http://hdl.handle.net/20.500.12110/paper_00224731_v28_n5_p543_Cozza
work_keys_str_mv	AT cozzaen convertibilityofasaponifiablelipoidalderivativeof18hydroxycorticosterone AT ceballosnr convertibilityofasaponifiablelipoidalderivativeof18hydroxycorticosterone AT delcarmenvilam convertibilityofasaponifiablelipoidalderivativeof18hydroxycorticosterone AT lantoscp convertibilityofasaponifiablelipoidalderivativeof18hydroxycorticosterone
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Convertibility of a saponifiable lipoidal derivative of 18-hydroxycorticosterone

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