Antibodies against epididymal glycoproteins block fertilizing ability in rat

Antiserum against rat androgen-dependent secretory epididymal protein DE (raised in rabbit) was added to suspensions of rat spermatozoa from the cauda epididymidis which were used for artificial insemination. While control spermatozoa fertilized 41.6% of oocytes, those exposed to antiserum to protei...

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Autores principales: Cuasnicu, P.S., Gonzalez Echeverria, F., Piazza, A.D., Cameo, M.S., Blaquier, J.A.
Formato: JOUR
Materias:
antibody
glycoprotein
sperm antibody
animal model
epididymis
fertilization
male genital system
nonhuman
priority journal
rat
Animalia
Oryctolagus cuniculus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00224251_v72_n2_p467_Cuasnicu
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00224251_v72_n2_p467_Cuasnicu
record_format dspace
spelling todo:paper_00224251_v72_n2_p467_Cuasnicu2023-10-03T14:32:53Z Antibodies against epididymal glycoproteins block fertilizing ability in rat Cuasnicu, P.S. Gonzalez Echeverria, F. Piazza, A.D. Cameo, M.S. Blaquier, J.A. antibody glycoprotein sperm antibody animal model epididymis fertilization male genital system nonhuman priority journal rat Animalia Oryctolagus cuniculus Antiserum against rat androgen-dependent secretory epididymal protein DE (raised in rabbit) was added to suspensions of rat spermatozoa from the cauda epididymidis which were used for artificial insemination. While control spermatozoa fertilized 41.6% of oocytes, those exposed to antiserum to protein DE fertilized only 6.6% (P < 0.01). An equal amount of normal rabbit serum (NRS) did not cause inhibition (33.1%). To study the entry of antibodies into the epididymis, caudal tubules were cultured for 24 h and the fertility of the contained spermatozoa was assessed by artificial insemination. Culture in Medium 199 alone or with NRS resulted in spermatozoa which fertilized 52% of oocytes while the presence of antiserum to protein DE in the culture medium yielded spermatozoa which fertilized only 16.6% of oocytes (P < 0.01). These results suggest (1) that the epididymal protein DE might be part of a sperm structure involved in the fertilization process, and (2) that, at least under the present culture conditions, immunoglobulins penetrate the epididymal epithelium in sufficient numbers to reduce fertility significantly. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00224251_v72_n2_p467_Cuasnicu
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic antibody
glycoprotein
sperm antibody
animal model
epididymis
fertilization
male genital system
nonhuman
priority journal
rat
Animalia
Oryctolagus cuniculus
spellingShingle antibody
glycoprotein
sperm antibody
animal model
epididymis
fertilization
male genital system
nonhuman
priority journal
rat
Animalia
Oryctolagus cuniculus
Cuasnicu, P.S.
Gonzalez Echeverria, F.
Piazza, A.D.
Cameo, M.S.
Blaquier, J.A.
Antibodies against epididymal glycoproteins block fertilizing ability in rat
topic_facet antibody
glycoprotein
sperm antibody
animal model
epididymis
fertilization
male genital system
nonhuman
priority journal
rat
Animalia
Oryctolagus cuniculus
description Antiserum against rat androgen-dependent secretory epididymal protein DE (raised in rabbit) was added to suspensions of rat spermatozoa from the cauda epididymidis which were used for artificial insemination. While control spermatozoa fertilized 41.6% of oocytes, those exposed to antiserum to protein DE fertilized only 6.6% (P < 0.01). An equal amount of normal rabbit serum (NRS) did not cause inhibition (33.1%). To study the entry of antibodies into the epididymis, caudal tubules were cultured for 24 h and the fertility of the contained spermatozoa was assessed by artificial insemination. Culture in Medium 199 alone or with NRS resulted in spermatozoa which fertilized 52% of oocytes while the presence of antiserum to protein DE in the culture medium yielded spermatozoa which fertilized only 16.6% of oocytes (P < 0.01). These results suggest (1) that the epididymal protein DE might be part of a sperm structure involved in the fertilization process, and (2) that, at least under the present culture conditions, immunoglobulins penetrate the epididymal epithelium in sufficient numbers to reduce fertility significantly.
format JOUR
author Cuasnicu, P.S.
Gonzalez Echeverria, F.
Piazza, A.D.
Cameo, M.S.
Blaquier, J.A.
author_facet Cuasnicu, P.S.
Gonzalez Echeverria, F.
Piazza, A.D.
Cameo, M.S.
Blaquier, J.A.
author_sort Cuasnicu, P.S.
title Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_short Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_full Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_fullStr Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_full_unstemmed Antibodies against epididymal glycoproteins block fertilizing ability in rat
title_sort antibodies against epididymal glycoproteins block fertilizing ability in rat
url http://hdl.handle.net/20.500.12110/paper_00224251_v72_n2_p467_Cuasnicu
work_keys_str_mv AT cuasnicups antibodiesagainstepididymalglycoproteinsblockfertilizingabilityinrat
AT gonzalezecheverriaf antibodiesagainstepididymalglycoproteinsblockfertilizingabilityinrat
AT piazzaad antibodiesagainstepididymalglycoproteinsblockfertilizingabilityinrat
AT cameoms antibodiesagainstepididymalglycoproteinsblockfertilizingabilityinrat
AT blaquierja antibodiesagainstepididymalglycoproteinsblockfertilizingabilityinrat
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