STED nanoscopy with wavelengths at the emission maximum
Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate th...
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Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00223727_v49_n36_p_Bordenave |
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todo:paper_00223727_v49_n36_p_Bordenave2023-10-03T14:32:26Z STED nanoscopy with wavelengths at the emission maximum Bordenave, M.D. Balzarotti, F. Stefani, F.D. Hell, S.W. acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums nanoscopy Stimulated emission depletion Sub-diffraction Super resolution Fluorophores Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission maximum, where the cross section for stimulated emission is up to 10-fold larger than at the red tail. As a result, STED imaging becomes possible at equally lower STED beam power. Besides, fluorophores that have been considered inapplicable in certain wavelength constellations are thus becoming usable. © 2016 IOP Publishing Ltd. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00223727_v49_n36_p_Bordenave |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums nanoscopy Stimulated emission depletion Sub-diffraction Super resolution Fluorophores |
spellingShingle |
acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums nanoscopy Stimulated emission depletion Sub-diffraction Super resolution Fluorophores Bordenave, M.D. Balzarotti, F. Stefani, F.D. Hell, S.W. STED nanoscopy with wavelengths at the emission maximum |
topic_facet |
acousto-optical devices fluorescence microscopy nanoscopy resolution superresolution Acoustooptical devices Emission spectroscopy Fluorescence Fluorescence microscopy Optical resolving power Stimulated emission Beam power Continuous waves Emission maxima Emission spectrums nanoscopy Stimulated emission depletion Sub-diffraction Super resolution Fluorophores |
description |
Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission maximum, where the cross section for stimulated emission is up to 10-fold larger than at the red tail. As a result, STED imaging becomes possible at equally lower STED beam power. Besides, fluorophores that have been considered inapplicable in certain wavelength constellations are thus becoming usable. © 2016 IOP Publishing Ltd. |
format |
JOUR |
author |
Bordenave, M.D. Balzarotti, F. Stefani, F.D. Hell, S.W. |
author_facet |
Bordenave, M.D. Balzarotti, F. Stefani, F.D. Hell, S.W. |
author_sort |
Bordenave, M.D. |
title |
STED nanoscopy with wavelengths at the emission maximum |
title_short |
STED nanoscopy with wavelengths at the emission maximum |
title_full |
STED nanoscopy with wavelengths at the emission maximum |
title_fullStr |
STED nanoscopy with wavelengths at the emission maximum |
title_full_unstemmed |
STED nanoscopy with wavelengths at the emission maximum |
title_sort |
sted nanoscopy with wavelengths at the emission maximum |
url |
http://hdl.handle.net/20.500.12110/paper_00223727_v49_n36_p_Bordenave |
work_keys_str_mv |
AT bordenavemd stednanoscopywithwavelengthsattheemissionmaximum AT balzarottif stednanoscopywithwavelengthsattheemissionmaximum AT stefanifd stednanoscopywithwavelengthsattheemissionmaximum AT hellsw stednanoscopywithwavelengthsattheemissionmaximum |
_version_ |
1782024060221259776 |