Cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta)

Cell walls in the coenocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) are composed of ∼32% (w/w) β-(1→4)-d-mannans, ∼12% sulfated polysaccharides (SPs), and small amounts of hydroxyproline-rich glycoprotein-like (HRGP-L) compounds of the arabinogalactan proteins (AGP...

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Autores principales: Fernández, P.V., Ciancia, M., Miravalles, A.B., Estevez, J.M.
Formato: JOUR
Materias:
β-(1→4)-d-mannans
Carbohydrate immunolabeling
Cell-wall arrangement
Chemical imaging
Codium vermilara
Coenocytic green seaweed
Hydroxyproline-rich glycoprotein-like
SR-FTIR microspectroscopy
Sulfated polysaccharides
Caulerpales
Chlorophyta
Codium
Codium fragile
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p456_Fernandez
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_00223646_v46_n3_p456_Fernandez2023-10-03T14:32:02Z Cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta) Fernández, P.V. Ciancia, M. Miravalles, A.B. Estevez, J.M. β-(1→4)-d-mannans Carbohydrate immunolabeling Cell-wall arrangement Chemical imaging Codium vermilara Coenocytic green seaweed Hydroxyproline-rich glycoprotein-like SR-FTIR microspectroscopy Sulfated polysaccharides Caulerpales Chlorophyta Codium Codium fragile Codium vermilara Cell walls in the coenocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) are composed of ∼32% (w/w) β-(1→4)-d-mannans, ∼12% sulfated polysaccharides (SPs), and small amounts of hydroxyproline-rich glycoprotein-like (HRGP-L) compounds of the arabinogalactan proteins (AGPs) and arabinosides (extensins). Similar quantities of mannans and SPs were reported previously in the related seaweed C. fragile (Suringar) Hariot. Overall, both seaweed cell walls comprise ∼40%-44% of their dry weights. Within the SP group, a variety of polysaccharide structures from pyruvylated arabinogalactan sulfate and pyruvylated galactan sulfate to pyranosic arabinan sulfate are present in Codium cell walls. In this paper, the in situ distribution of the main cell-wall polymers in the green seaweed C. vermilara was studied, comparing their arrangements with those observed in cell walls from C. fragile. The utricle cell wall in C. vermilara showed by TEM a sandwich structure of two fibrillar-like layers of similar width delimiting a middle amorphous-like zone. By immuno- and chemical imaging, the in situ distribution of β-(1→4)-d-mannans and HRGP-like epitopes was shown to consist of two distinct cell-wall layers, whereas SPs are distributed in the middle area of the wall. The overall cell-wall polymer arrangement of the SPs, HRGP-like epitopes, and mannans in the utricles of C. vermilara is different from the ubiquitous green algae C. fragile, in spite of both being phylogenetically very close. In addition, a preliminary cell-wall model of the utricle moiety is proposed for both seaweeds, C. fragile and C. vermilara. © 2010 Phycological Society of America. Fil:Fernández, P.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ciancia, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Estevez, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p456_Fernandez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic β-(1→4)-d-mannans
Carbohydrate immunolabeling
Cell-wall arrangement
Chemical imaging
Codium vermilara
Coenocytic green seaweed
Hydroxyproline-rich glycoprotein-like
SR-FTIR microspectroscopy
Sulfated polysaccharides
Caulerpales
Chlorophyta
Codium
Codium fragile
Codium vermilara
spellingShingle β-(1→4)-d-mannans
Carbohydrate immunolabeling
Cell-wall arrangement
Chemical imaging
Codium vermilara
Coenocytic green seaweed
Hydroxyproline-rich glycoprotein-like
SR-FTIR microspectroscopy
Sulfated polysaccharides
Caulerpales
Chlorophyta
Codium
Codium fragile
Codium vermilara
Fernández, P.V.
Ciancia, M.
Miravalles, A.B.
Estevez, J.M.
Cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta)
topic_facet β-(1→4)-d-mannans
Carbohydrate immunolabeling
Cell-wall arrangement
Chemical imaging
Codium vermilara
Coenocytic green seaweed
Hydroxyproline-rich glycoprotein-like
SR-FTIR microspectroscopy
Sulfated polysaccharides
Caulerpales
Chlorophyta
Codium
Codium fragile
Codium vermilara
description Cell walls in the coenocytic green seaweed Codium vermilara (Olivi) Chiaje (Bryopsidales, Chlorophyta) are composed of ∼32% (w/w) β-(1→4)-d-mannans, ∼12% sulfated polysaccharides (SPs), and small amounts of hydroxyproline-rich glycoprotein-like (HRGP-L) compounds of the arabinogalactan proteins (AGPs) and arabinosides (extensins). Similar quantities of mannans and SPs were reported previously in the related seaweed C. fragile (Suringar) Hariot. Overall, both seaweed cell walls comprise ∼40%-44% of their dry weights. Within the SP group, a variety of polysaccharide structures from pyruvylated arabinogalactan sulfate and pyruvylated galactan sulfate to pyranosic arabinan sulfate are present in Codium cell walls. In this paper, the in situ distribution of the main cell-wall polymers in the green seaweed C. vermilara was studied, comparing their arrangements with those observed in cell walls from C. fragile. The utricle cell wall in C. vermilara showed by TEM a sandwich structure of two fibrillar-like layers of similar width delimiting a middle amorphous-like zone. By immuno- and chemical imaging, the in situ distribution of β-(1→4)-d-mannans and HRGP-like epitopes was shown to consist of two distinct cell-wall layers, whereas SPs are distributed in the middle area of the wall. The overall cell-wall polymer arrangement of the SPs, HRGP-like epitopes, and mannans in the utricles of C. vermilara is different from the ubiquitous green algae C. fragile, in spite of both being phylogenetically very close. In addition, a preliminary cell-wall model of the utricle moiety is proposed for both seaweeds, C. fragile and C. vermilara. © 2010 Phycological Society of America.
format JOUR
author Fernández, P.V.
Ciancia, M.
Miravalles, A.B.
Estevez, J.M.
author_facet Fernández, P.V.
Ciancia, M.
Miravalles, A.B.
Estevez, J.M.
author_sort Fernández, P.V.
title Cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta)
title_short Cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta)
title_full Cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta)
title_fullStr Cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta)
title_full_unstemmed Cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta)
title_sort cell-wall polymer mapping in the coenocytic macroalga codium vermilara (bryopsidales, chlorophyta)
url http://hdl.handle.net/20.500.12110/paper_00223646_v46_n3_p456_Fernandez
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AT cianciam cellwallpolymermappinginthecoenocyticmacroalgacodiumvermilarabryopsidaleschlorophyta
AT miravallesab cellwallpolymermappinginthecoenocyticmacroalgacodiumvermilarabryopsidaleschlorophyta
AT estevezjm cellwallpolymermappinginthecoenocyticmacroalgacodiumvermilarabryopsidaleschlorophyta
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