Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei
Methylphenidate (MPH) is widely used to treat children and adolescents diagnosed with attention deficit/hyperactivity disorder. Although MPH shares mechanistic similarities to cocaine, its effects on GABAergic transmission in sensory thalamic nuclei are unknown. Our objective was to compare cocaine...
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todo:paper_00223042_v124_n5_p602_Goitia2023-10-03T14:30:49Z Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei Goitia, B. Raineri, M. González, L.E. Rozas, J.L. Garcia-Rill, E. Bisagno, V. Urbano, F.J. cocaine GABAergic transmission methylphenidate T-type calcium channels thalamic reticular nucleus ventrobasal thalamic nucleus 4 aminobutyric acid calcium channel T type calcium ion cocaine glutamate decarboxylase 67 methylphenidate 4 aminobutyric acid release animal cell animal experiment animal model animal tissue article calcium current controlled study facilitation GABAergic transmission locomotion male mouse nerve cell inhibition nerve projection nonhuman postsynaptic membrane priority journal thalamus reticular nucleus thalamus ventral nucleus Western blotting Animals Blotting, Western Central Nervous System Stimulants Cocaine gamma-Aminobutyric Acid Male Methylphenidate Mice Mice, Inbred C57BL Patch-Clamp Techniques Synaptic Transmission Thalamic Nuclei Mus Methylphenidate (MPH) is widely used to treat children and adolescents diagnosed with attention deficit/hyperactivity disorder. Although MPH shares mechanistic similarities to cocaine, its effects on GABAergic transmission in sensory thalamic nuclei are unknown. Our objective was to compare cocaine and MPH effects on GABAergic projections between thalamic reticular and ventrobasal (VB) nuclei. Mice (P18-30) were subjected to binge-like cocaine and MPH acute and sub-chronic administrations. Cocaine and MPH enhanced hyperlocomotion, although sub-chronic cocaine-mediated effects were stronger than MPH effects. Cocaine and MPH sub-chronic administration altered paired-pulse and spontaneous GABAergic input differently. The effects of cocaine on evoked paired-pulse GABA-mediated currents changed from depression to facilitation with the duration of the protocols used, while MPH induced a constant increase throughout the administration protocols. Thalamic reticular nucleus GAD67 and VB Ca V3.1 protein levels were measured using western blot to better understand their link to increased GABA release. Both proteins were increased by sub-chronic administration of cocaine. MPH showed effects on GABAergic transmission that seems less disruptive than cocaine. Unique effects of cocaine on postsynaptic VB calcium currents might explain deleterious cocaine effects on sensory thalamic nuclei. These results suggest that cocaine and MPH produced distinct presynaptic alterations on GABAergic transmission. © 2012 International Society for Neurochemistry. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00223042_v124_n5_p602_Goitia |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cocaine GABAergic transmission methylphenidate T-type calcium channels thalamic reticular nucleus ventrobasal thalamic nucleus 4 aminobutyric acid calcium channel T type calcium ion cocaine glutamate decarboxylase 67 methylphenidate 4 aminobutyric acid release animal cell animal experiment animal model animal tissue article calcium current controlled study facilitation GABAergic transmission locomotion male mouse nerve cell inhibition nerve projection nonhuman postsynaptic membrane priority journal thalamus reticular nucleus thalamus ventral nucleus Western blotting Animals Blotting, Western Central Nervous System Stimulants Cocaine gamma-Aminobutyric Acid Male Methylphenidate Mice Mice, Inbred C57BL Patch-Clamp Techniques Synaptic Transmission Thalamic Nuclei Mus |
| spellingShingle |
cocaine GABAergic transmission methylphenidate T-type calcium channels thalamic reticular nucleus ventrobasal thalamic nucleus 4 aminobutyric acid calcium channel T type calcium ion cocaine glutamate decarboxylase 67 methylphenidate 4 aminobutyric acid release animal cell animal experiment animal model animal tissue article calcium current controlled study facilitation GABAergic transmission locomotion male mouse nerve cell inhibition nerve projection nonhuman postsynaptic membrane priority journal thalamus reticular nucleus thalamus ventral nucleus Western blotting Animals Blotting, Western Central Nervous System Stimulants Cocaine gamma-Aminobutyric Acid Male Methylphenidate Mice Mice, Inbred C57BL Patch-Clamp Techniques Synaptic Transmission Thalamic Nuclei Mus Goitia, B. Raineri, M. González, L.E. Rozas, J.L. Garcia-Rill, E. Bisagno, V. Urbano, F.J. Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei |
| topic_facet |
cocaine GABAergic transmission methylphenidate T-type calcium channels thalamic reticular nucleus ventrobasal thalamic nucleus 4 aminobutyric acid calcium channel T type calcium ion cocaine glutamate decarboxylase 67 methylphenidate 4 aminobutyric acid release animal cell animal experiment animal model animal tissue article calcium current controlled study facilitation GABAergic transmission locomotion male mouse nerve cell inhibition nerve projection nonhuman postsynaptic membrane priority journal thalamus reticular nucleus thalamus ventral nucleus Western blotting Animals Blotting, Western Central Nervous System Stimulants Cocaine gamma-Aminobutyric Acid Male Methylphenidate Mice Mice, Inbred C57BL Patch-Clamp Techniques Synaptic Transmission Thalamic Nuclei Mus |
| description |
Methylphenidate (MPH) is widely used to treat children and adolescents diagnosed with attention deficit/hyperactivity disorder. Although MPH shares mechanistic similarities to cocaine, its effects on GABAergic transmission in sensory thalamic nuclei are unknown. Our objective was to compare cocaine and MPH effects on GABAergic projections between thalamic reticular and ventrobasal (VB) nuclei. Mice (P18-30) were subjected to binge-like cocaine and MPH acute and sub-chronic administrations. Cocaine and MPH enhanced hyperlocomotion, although sub-chronic cocaine-mediated effects were stronger than MPH effects. Cocaine and MPH sub-chronic administration altered paired-pulse and spontaneous GABAergic input differently. The effects of cocaine on evoked paired-pulse GABA-mediated currents changed from depression to facilitation with the duration of the protocols used, while MPH induced a constant increase throughout the administration protocols. Thalamic reticular nucleus GAD67 and VB Ca V3.1 protein levels were measured using western blot to better understand their link to increased GABA release. Both proteins were increased by sub-chronic administration of cocaine. MPH showed effects on GABAergic transmission that seems less disruptive than cocaine. Unique effects of cocaine on postsynaptic VB calcium currents might explain deleterious cocaine effects on sensory thalamic nuclei. These results suggest that cocaine and MPH produced distinct presynaptic alterations on GABAergic transmission. © 2012 International Society for Neurochemistry. |
| format |
JOUR |
| author |
Goitia, B. Raineri, M. González, L.E. Rozas, J.L. Garcia-Rill, E. Bisagno, V. Urbano, F.J. |
| author_facet |
Goitia, B. Raineri, M. González, L.E. Rozas, J.L. Garcia-Rill, E. Bisagno, V. Urbano, F.J. |
| author_sort |
Goitia, B. |
| title |
Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei |
| title_short |
Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei |
| title_full |
Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei |
| title_fullStr |
Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei |
| title_full_unstemmed |
Differential effects of methylphenidate and cocaine on GABA transmission in sensory thalamic nuclei |
| title_sort |
differential effects of methylphenidate and cocaine on gaba transmission in sensory thalamic nuclei |
| url |
http://hdl.handle.net/20.500.12110/paper_00223042_v124_n5_p602_Goitia |
| work_keys_str_mv |
AT goitiab differentialeffectsofmethylphenidateandcocaineongabatransmissioninsensorythalamicnuclei AT rainerim differentialeffectsofmethylphenidateandcocaineongabatransmissioninsensorythalamicnuclei AT gonzalezle differentialeffectsofmethylphenidateandcocaineongabatransmissioninsensorythalamicnuclei AT rozasjl differentialeffectsofmethylphenidateandcocaineongabatransmissioninsensorythalamicnuclei AT garciarille differentialeffectsofmethylphenidateandcocaineongabatransmissioninsensorythalamicnuclei AT bisagnov differentialeffectsofmethylphenidateandcocaineongabatransmissioninsensorythalamicnuclei AT urbanofj differentialeffectsofmethylphenidateandcocaineongabatransmissioninsensorythalamicnuclei |
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