Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit
The cell cycle has gained attention as a key determinant for cell fate decisions, but the contribution of DNA replication and mitosis in stem cell differentiation has not been extensively studied. To understand if these processes act as “windows of opportunity” for changes in cell identity, we estab...
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todo:paper_00222836_v429_n18_p2802_Waisman2023-10-03T14:30:24Z Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit Waisman, A. Vazquez Echegaray, C. Solari, C. Cosentino, M.S. Martyn, I. Deglincerti, A. Ozair, M.Z. Ruzo, A. Barañao, L. Miriuka, S. Brivanlou, A. Guberman, A. cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation cell division controlled study DNA replication mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal cell differentiation genetic transcription physiology pluripotent stem cell Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic The cell cycle has gained attention as a key determinant for cell fate decisions, but the contribution of DNA replication and mitosis in stem cell differentiation has not been extensively studied. To understand if these processes act as “windows of opportunity” for changes in cell identity, we established synchronized cultures of mouse embryonic stem cells as they exit the ground state of pluripotency. We show that initial transcriptional changes in this transition do not require passage through mitosis and that conversion to primed pluripotency is linked to lineage priming in the G1 phase. Importantly, we demonstrate that impairment of DNA replication severely blocks transcriptional switch to primed pluripotency, even in the absence of p53 activity induced by the DNA damage response. Our data suggest an important role for DNA replication during mouse embryonic stem cell differentiation, which could shed light on why pluripotent cells are only receptive to differentiation signals during G1, that is, before the S phase. © 2017 Elsevier Ltd Fil:Waisman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cosentino, M.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guberman, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00222836_v429_n18_p2802_Waisman |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation cell division controlled study DNA replication mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal cell differentiation genetic transcription physiology pluripotent stem cell Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic |
| spellingShingle |
cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation cell division controlled study DNA replication mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal cell differentiation genetic transcription physiology pluripotent stem cell Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic Waisman, A. Vazquez Echegaray, C. Solari, C. Cosentino, M.S. Martyn, I. Deglincerti, A. Ozair, M.Z. Ruzo, A. Barañao, L. Miriuka, S. Brivanlou, A. Guberman, A. Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit |
| topic_facet |
cell division DNA replication mouse embryonic stem cells introduction primitive ectoderm-like cells DNA methyltransferase 3A kruppel like factor 4 octamer transcription factor 6 protein p53 transcription factor NANOG transcription factor Otx2 animal cell Article cell cycle cell cycle G1 phase cell cycle regulation cell cycle S phase cell differentiation cell division controlled study DNA replication mitosis mouse mouse embryonic stem cell nonhuman pluripotent stem cell priority journal stem cell animal cell differentiation genetic transcription physiology pluripotent stem cell Animals Cell Differentiation Cell Division DNA Replication Mice Mouse Embryonic Stem Cells Pluripotent Stem Cells Transcription, Genetic |
| description |
The cell cycle has gained attention as a key determinant for cell fate decisions, but the contribution of DNA replication and mitosis in stem cell differentiation has not been extensively studied. To understand if these processes act as “windows of opportunity” for changes in cell identity, we established synchronized cultures of mouse embryonic stem cells as they exit the ground state of pluripotency. We show that initial transcriptional changes in this transition do not require passage through mitosis and that conversion to primed pluripotency is linked to lineage priming in the G1 phase. Importantly, we demonstrate that impairment of DNA replication severely blocks transcriptional switch to primed pluripotency, even in the absence of p53 activity induced by the DNA damage response. Our data suggest an important role for DNA replication during mouse embryonic stem cell differentiation, which could shed light on why pluripotent cells are only receptive to differentiation signals during G1, that is, before the S phase. © 2017 Elsevier Ltd |
| format |
JOUR |
| author |
Waisman, A. Vazquez Echegaray, C. Solari, C. Cosentino, M.S. Martyn, I. Deglincerti, A. Ozair, M.Z. Ruzo, A. Barañao, L. Miriuka, S. Brivanlou, A. Guberman, A. |
| author_facet |
Waisman, A. Vazquez Echegaray, C. Solari, C. Cosentino, M.S. Martyn, I. Deglincerti, A. Ozair, M.Z. Ruzo, A. Barañao, L. Miriuka, S. Brivanlou, A. Guberman, A. |
| author_sort |
Waisman, A. |
| title |
Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit |
| title_short |
Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit |
| title_full |
Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit |
| title_fullStr |
Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit |
| title_full_unstemmed |
Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit |
| title_sort |
inhibition of cell division and dna replication impair mouse-naïve pluripotency exit |
| url |
http://hdl.handle.net/20.500.12110/paper_00222836_v429_n18_p2802_Waisman |
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