The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster
This report shows the biochemical characterization and life cycle-dependent expression of Drosophila melanogaster N-β-alanyldopamine synthase (NBAD-synthase or Ebony protein). This enzyme not only catalyzes the synthesis of NBAD, the main sclerotization and pigmentation precursor of insect brown cut...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00221910_v56_n1_p8_Perez |
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todo:paper_00221910_v56_n1_p8_Perez2023-10-03T14:28:18Z The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster Pérez, M.M. Schachter, J. Berni, J. Quesada-Allué, L.A. Cuticle Insect brain Neurotransmitters Sclerotization Tracheal development DNA binding protein Drosophila protein ebony protein, Drosophila biochemistry cuticle enzyme activity fly life cycle metabolism animal article Drosophila melanogaster enzymology epidermis female gastrointestinal tract immunohistochemistry life cycle metabolism nervous system prenatal development trachea Animals DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Epidermis Female Gastrointestinal Tract Immunohistochemistry Life Cycle Stages Nervous System Trachea Drosophila melanogaster Hexapoda This report shows the biochemical characterization and life cycle-dependent expression of Drosophila melanogaster N-β-alanyldopamine synthase (NBAD-synthase or Ebony protein). This enzyme not only catalyzes the synthesis of NBAD, the main sclerotization and pigmentation precursor of insect brown cuticles, but also plays a role in brain neurotransmitter metabolism. In addition to the epidermis expression our immunodetection experiments show the novel localization of NBAD-synthase in different regions of the adult brain, in the foregut of pharate adult and, surprisingly, in the epidermis of the trachea during embryogenesis. These results demonstrate that NBAD-synthase is a versatile enzyme involved in different, previously unknown, time- and tissue-dependent processes. © 2009 Elsevier Ltd. All rights reserved. Fil:Pérez, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Berni, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Quesada-Allué, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221910_v56_n1_p8_Perez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cuticle Insect brain Neurotransmitters Sclerotization Tracheal development DNA binding protein Drosophila protein ebony protein, Drosophila biochemistry cuticle enzyme activity fly life cycle metabolism animal article Drosophila melanogaster enzymology epidermis female gastrointestinal tract immunohistochemistry life cycle metabolism nervous system prenatal development trachea Animals DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Epidermis Female Gastrointestinal Tract Immunohistochemistry Life Cycle Stages Nervous System Trachea Drosophila melanogaster Hexapoda |
| spellingShingle |
Cuticle Insect brain Neurotransmitters Sclerotization Tracheal development DNA binding protein Drosophila protein ebony protein, Drosophila biochemistry cuticle enzyme activity fly life cycle metabolism animal article Drosophila melanogaster enzymology epidermis female gastrointestinal tract immunohistochemistry life cycle metabolism nervous system prenatal development trachea Animals DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Epidermis Female Gastrointestinal Tract Immunohistochemistry Life Cycle Stages Nervous System Trachea Drosophila melanogaster Hexapoda Pérez, M.M. Schachter, J. Berni, J. Quesada-Allué, L.A. The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster |
| topic_facet |
Cuticle Insect brain Neurotransmitters Sclerotization Tracheal development DNA binding protein Drosophila protein ebony protein, Drosophila biochemistry cuticle enzyme activity fly life cycle metabolism animal article Drosophila melanogaster enzymology epidermis female gastrointestinal tract immunohistochemistry life cycle metabolism nervous system prenatal development trachea Animals DNA-Binding Proteins Drosophila melanogaster Drosophila Proteins Epidermis Female Gastrointestinal Tract Immunohistochemistry Life Cycle Stages Nervous System Trachea Drosophila melanogaster Hexapoda |
| description |
This report shows the biochemical characterization and life cycle-dependent expression of Drosophila melanogaster N-β-alanyldopamine synthase (NBAD-synthase or Ebony protein). This enzyme not only catalyzes the synthesis of NBAD, the main sclerotization and pigmentation precursor of insect brown cuticles, but also plays a role in brain neurotransmitter metabolism. In addition to the epidermis expression our immunodetection experiments show the novel localization of NBAD-synthase in different regions of the adult brain, in the foregut of pharate adult and, surprisingly, in the epidermis of the trachea during embryogenesis. These results demonstrate that NBAD-synthase is a versatile enzyme involved in different, previously unknown, time- and tissue-dependent processes. © 2009 Elsevier Ltd. All rights reserved. |
| format |
JOUR |
| author |
Pérez, M.M. Schachter, J. Berni, J. Quesada-Allué, L.A. |
| author_facet |
Pérez, M.M. Schachter, J. Berni, J. Quesada-Allué, L.A. |
| author_sort |
Pérez, M.M. |
| title |
The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster |
| title_short |
The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster |
| title_full |
The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster |
| title_fullStr |
The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster |
| title_full_unstemmed |
The enzyme NBAD-synthase plays diverse roles during the life cycle of Drosophila melanogaster |
| title_sort |
enzyme nbad-synthase plays diverse roles during the life cycle of drosophila melanogaster |
| url |
http://hdl.handle.net/20.500.12110/paper_00221910_v56_n1_p8_Perez |
| work_keys_str_mv |
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1807317548327239680 |