Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons
The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity...
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todo:paper_00221333_v90_n1_p111_Nieves2023-10-03T14:27:30Z Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons Nieves, M. De Oliveira, E.H.C. Amaral, P.J.S. Nagamachi, C.Y. Pieczarka, J.C. Mühlmann, M.C. Mudry, M.D. Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article Cebus chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics heterochromatin karyotyping microdissection nucleotide repeat Animals Cebus Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Platyrrhini Primates The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus. We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences. © 2011 Indian Academy of Sciences. Fil:Nieves, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221333_v90_n1_p111_Nieves |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article Cebus chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics heterochromatin karyotyping microdissection nucleotide repeat Animals Cebus Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Platyrrhini Primates |
| spellingShingle |
Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article Cebus chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics heterochromatin karyotyping microdissection nucleotide repeat Animals Cebus Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Platyrrhini Primates Nieves, M. De Oliveira, E.H.C. Amaral, P.J.S. Nagamachi, C.Y. Pieczarka, J.C. Mühlmann, M.C. Mudry, M.D. Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons |
| topic_facet |
Cebus chromosome microdissection FISH heterochromatin Platyrrhini primate evolution animal article Cebus chromosome chromosome banding pattern comparative study fluorescence in situ hybridization genetics heterochromatin karyotyping microdissection nucleotide repeat Animals Cebus Chromosome Banding Chromosomes Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Microdissection Repetitive Sequences, Nucleic Acid Callitrichidae Cebidae Cebus Cebus albifrons Cebus apella Cebus kaapori Cebus libidinosus Cebus olivaceus Cebus xanthosternos Platyrrhini Primates |
| description |
The karyotype of the neotropical primate genus Cebus (Platyrrhini: Cebidae), considered the most ancestral one, shows the greatest amount of heterochromatin described among Platyrrhini genera. Banding techniques and restriction enzyme digestion have previously revealed great variability of quantity and composition of heterochromatin in this genus. In this context, we use fluorescence in situ hybridization (FISH) to analyse this genomic region and discuss its possible role in the diversification of Cebus. We used a heterochromatin probe for chromosome 11 of Cebus libidinosus (11qHe+ CLI probe), obtained by chromosome microdissection. Twenty-six specimens belonging to the families Atelidae, Cebidae, Callitrichidae and Pithecidae (Platyrrhini) were studied. Fourteen out of 26 specimens were Cebus (Cebidae) individuals of C. libidinosus, C. xanthosternos, C. apella, C. nigritus, C. albifrons, C. kaapori and C. olivaceus. In Cebus specimens, we found 6 to 22 positive signals located in interstitial and telomeric positions along the different species. No hybridization signal was observed among the remaining Ceboidea species, thus reinforcing the idea of a Cebus-specific heterochromatin composed of a complex system of repetitive sequences. © 2011 Indian Academy of Sciences. |
| format |
JOUR |
| author |
Nieves, M. De Oliveira, E.H.C. Amaral, P.J.S. Nagamachi, C.Y. Pieczarka, J.C. Mühlmann, M.C. Mudry, M.D. |
| author_facet |
Nieves, M. De Oliveira, E.H.C. Amaral, P.J.S. Nagamachi, C.Y. Pieczarka, J.C. Mühlmann, M.C. Mudry, M.D. |
| author_sort |
Nieves, M. |
| title |
Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons |
| title_short |
Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons |
| title_full |
Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons |
| title_fullStr |
Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons |
| title_full_unstemmed |
Analysis of the heterochromatin of Cebus (Primates, Platyrrhini) by micro-FISH and banding pattern comparisons |
| title_sort |
analysis of the heterochromatin of cebus (primates, platyrrhini) by micro-fish and banding pattern comparisons |
| url |
http://hdl.handle.net/20.500.12110/paper_00221333_v90_n1_p111_Nieves |
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