Superinfection exclusion in BHK-21 cells persistently infected with Junín virus
We characterized a persistently Junin virus (JUNV)-infected BHK-21 cell line obtained by experimental infection with the XJC13 strain. This cell line, named K3, produced low levels of virus in supernatants which were not influenced by the presence of defective interfering (DI) particles after the fi...
Guardado en:
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00221317_v88_n10_p2730_Ellenberg |
| Aporte de: |
| id |
todo:paper_00221317_v88_n10_p2730_Ellenberg |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00221317_v88_n10_p2730_Ellenberg2023-10-03T14:27:24Z Superinfection exclusion in BHK-21 cells persistently infected with Junín virus Ellenberg, P. Linero, F.N. Scolaro, L.A. cell protein virus antigen virus protein virus RNA animal cell antigenicity Arenavirus article biosynthesis cell budding cell count cell isolation cell membrane cell strain BHK controlled study correlation analysis gene overexpression Junin virus Lymphocytic choriomeningitis virus molecular cloning nonhuman Pichinde arenavirus priority journal superinfection supernatant virus infection virus particle virus replication virus strain Animals Antigens, Viral Arenaviridae Infections Cell Line Cercopithecus aethiops Cricetinae Defective Viruses DNA Primers DNA, Complementary Genome, Viral Haplorhini Junin virus Kidney Polymerase Chain Reaction RNA, Viral Superinfection Vero Cells Arenavirus Junin virus Lymphocytic choriomeningitis virus Pichinde virus RNA viruses Tacaribe virus We characterized a persistently Junin virus (JUNV)-infected BHK-21 cell line obtained by experimental infection with the XJC13 strain. This cell line, named K3, produced low levels of virus in supernatants which were not influenced by the presence of defective interfering (DI) particles after the first year of infection. K3 cells were able to exclude superinfection of the homologous JUNV and the antigenically related Tacaribe virus (TCRV), whereas the non-related arenaviruses lymphocytic choriomeningitis virus (LCMV) and Pichinde virus (PICV) could replicate normally. Although superinfecting virus binding and internalization to persistently infected cells were slightly reduced, earlier biosynthesis of antigenomic RNA was observed in comparison with BHK-21 cells. Despite the fact that superinfection did not increase the number of cells expressing viral antigens, de novo synthesis of superinfecting virus proteins was detected. The virus produced by JUNV-superinfected K3 cells remained mostly cell-associated in the form of particles tethered to the plasma membrane and aberrant tubular structures. JUNV restriction was correlated with an overexpression of cellular protein TSG101 in K3 cells, which has been pointed out as involved in the budding of several RNA viruses. This correlation was also observed in a cell clone isolated from K3. Reduction of TSG101 expression favoured the release of infectious virus to the supernatant of JUNV-superinfected K3 cells. Our data suggest that overexpression of TSG101 in K3 cells is a novel mechanism that may contribute, along with a diminished synthesis of superinfecting virus proteins, to explain superinfection exclusion in persistently arenavirus-infected cells. © 2007 SGM. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221317_v88_n10_p2730_Ellenberg |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cell protein virus antigen virus protein virus RNA animal cell antigenicity Arenavirus article biosynthesis cell budding cell count cell isolation cell membrane cell strain BHK controlled study correlation analysis gene overexpression Junin virus Lymphocytic choriomeningitis virus molecular cloning nonhuman Pichinde arenavirus priority journal superinfection supernatant virus infection virus particle virus replication virus strain Animals Antigens, Viral Arenaviridae Infections Cell Line Cercopithecus aethiops Cricetinae Defective Viruses DNA Primers DNA, Complementary Genome, Viral Haplorhini Junin virus Kidney Polymerase Chain Reaction RNA, Viral Superinfection Vero Cells Arenavirus Junin virus Lymphocytic choriomeningitis virus Pichinde virus RNA viruses Tacaribe virus |
| spellingShingle |
cell protein virus antigen virus protein virus RNA animal cell antigenicity Arenavirus article biosynthesis cell budding cell count cell isolation cell membrane cell strain BHK controlled study correlation analysis gene overexpression Junin virus Lymphocytic choriomeningitis virus molecular cloning nonhuman Pichinde arenavirus priority journal superinfection supernatant virus infection virus particle virus replication virus strain Animals Antigens, Viral Arenaviridae Infections Cell Line Cercopithecus aethiops Cricetinae Defective Viruses DNA Primers DNA, Complementary Genome, Viral Haplorhini Junin virus Kidney Polymerase Chain Reaction RNA, Viral Superinfection Vero Cells Arenavirus Junin virus Lymphocytic choriomeningitis virus Pichinde virus RNA viruses Tacaribe virus Ellenberg, P. Linero, F.N. Scolaro, L.A. Superinfection exclusion in BHK-21 cells persistently infected with Junín virus |
| topic_facet |
cell protein virus antigen virus protein virus RNA animal cell antigenicity Arenavirus article biosynthesis cell budding cell count cell isolation cell membrane cell strain BHK controlled study correlation analysis gene overexpression Junin virus Lymphocytic choriomeningitis virus molecular cloning nonhuman Pichinde arenavirus priority journal superinfection supernatant virus infection virus particle virus replication virus strain Animals Antigens, Viral Arenaviridae Infections Cell Line Cercopithecus aethiops Cricetinae Defective Viruses DNA Primers DNA, Complementary Genome, Viral Haplorhini Junin virus Kidney Polymerase Chain Reaction RNA, Viral Superinfection Vero Cells Arenavirus Junin virus Lymphocytic choriomeningitis virus Pichinde virus RNA viruses Tacaribe virus |
| description |
We characterized a persistently Junin virus (JUNV)-infected BHK-21 cell line obtained by experimental infection with the XJC13 strain. This cell line, named K3, produced low levels of virus in supernatants which were not influenced by the presence of defective interfering (DI) particles after the first year of infection. K3 cells were able to exclude superinfection of the homologous JUNV and the antigenically related Tacaribe virus (TCRV), whereas the non-related arenaviruses lymphocytic choriomeningitis virus (LCMV) and Pichinde virus (PICV) could replicate normally. Although superinfecting virus binding and internalization to persistently infected cells were slightly reduced, earlier biosynthesis of antigenomic RNA was observed in comparison with BHK-21 cells. Despite the fact that superinfection did not increase the number of cells expressing viral antigens, de novo synthesis of superinfecting virus proteins was detected. The virus produced by JUNV-superinfected K3 cells remained mostly cell-associated in the form of particles tethered to the plasma membrane and aberrant tubular structures. JUNV restriction was correlated with an overexpression of cellular protein TSG101 in K3 cells, which has been pointed out as involved in the budding of several RNA viruses. This correlation was also observed in a cell clone isolated from K3. Reduction of TSG101 expression favoured the release of infectious virus to the supernatant of JUNV-superinfected K3 cells. Our data suggest that overexpression of TSG101 in K3 cells is a novel mechanism that may contribute, along with a diminished synthesis of superinfecting virus proteins, to explain superinfection exclusion in persistently arenavirus-infected cells. © 2007 SGM. |
| format |
JOUR |
| author |
Ellenberg, P. Linero, F.N. Scolaro, L.A. |
| author_facet |
Ellenberg, P. Linero, F.N. Scolaro, L.A. |
| author_sort |
Ellenberg, P. |
| title |
Superinfection exclusion in BHK-21 cells persistently infected with Junín virus |
| title_short |
Superinfection exclusion in BHK-21 cells persistently infected with Junín virus |
| title_full |
Superinfection exclusion in BHK-21 cells persistently infected with Junín virus |
| title_fullStr |
Superinfection exclusion in BHK-21 cells persistently infected with Junín virus |
| title_full_unstemmed |
Superinfection exclusion in BHK-21 cells persistently infected with Junín virus |
| title_sort |
superinfection exclusion in bhk-21 cells persistently infected with junín virus |
| url |
http://hdl.handle.net/20.500.12110/paper_00221317_v88_n10_p2730_Ellenberg |
| work_keys_str_mv |
AT ellenbergp superinfectionexclusioninbhk21cellspersistentlyinfectedwithjuninvirus AT linerofn superinfectionexclusioninbhk21cellspersistentlyinfectedwithjuninvirus AT scolarola superinfectionexclusioninbhk21cellspersistentlyinfectedwithjuninvirus |
| _version_ |
1807320577264844800 |