Involvement of the cytoskeleton in Junin virus multiplication

The role of the cellular cytoskeleton in Junin virus (JV) infection was explored in two ways. Firstly, the action of inhibitors that affect individual cytoskeletal systems (microtubules or microfilaments) selectively was analysed. It was found that perturbations of microtubule or microfilament netwo...

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Autores principales:	Candurra, N.A., Lago, M.J., Maskin, L., Damonte, E.B.
Formato:	JOUR
Materias:	colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia RNA viruses
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00221317_v80_n1_p147_Candurra
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00221317_v80_n1_p147_Candurra
record_format	dspace
spelling	todo:paper_00221317_v80_n1_p147_Candurra2023-10-03T14:27:23Z Involvement of the cytoskeleton in Junin virus multiplication Candurra, N.A. Lago, M.J. Maskin, L. Damonte, E.B. colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia Junin virus RNA viruses The role of the cellular cytoskeleton in Junin virus (JV) infection was explored in two ways. Firstly, the action of inhibitors that affect individual cytoskeletal systems (microtubules or microfilaments) selectively was analysed. It was found that perturbations of microtubule or microfilament networks caused by colchicine, nocodazole, nifedipine, EGTA or DMSO strongly affected virion production and viral protein expression at non-cytotoxic concentrations. Secondly, the extent of association of viral proteins and infectious virus particles with the cytoskeletal fraction of monkey Vero cells was determined by using three non-ionic detergents, Triton X-100 (TX-100), NP-40 and octyl glucoside (OG). The cytoskeleton retained nearly 70% of the external JV envelope glycoprotein GP38 and about 40% of the JV nucleoprotein NP, according to TX-100 and OG insolubility results. Furthermore, 1% of the total cell-bound infectivity was detected in the detergent-insoluble fraction, suggesting that cytoskeletal components are involved in the initiation of the assembly and budding processes of JV particles at the plasma membrane. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lago, M.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maskin, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221317_v80_n1_p147_Candurra
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia Junin virus RNA viruses
spellingShingle	colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia Junin virus RNA viruses Candurra, N.A. Lago, M.J. Maskin, L. Damonte, E.B. Involvement of the cytoskeleton in Junin virus multiplication
topic_facet	colchicine dimethyl sulfoxide egtazic acid nifedipine nocodazole octyl glucoside triton x 100 virus envelope protein virus glycoprotein virus protein animal cell article cytoskeleton Junin virus microfilament microtubule nonhuman priority journal Vero cell virogenesis Animalia Junin virus RNA viruses
description	The role of the cellular cytoskeleton in Junin virus (JV) infection was explored in two ways. Firstly, the action of inhibitors that affect individual cytoskeletal systems (microtubules or microfilaments) selectively was analysed. It was found that perturbations of microtubule or microfilament networks caused by colchicine, nocodazole, nifedipine, EGTA or DMSO strongly affected virion production and viral protein expression at non-cytotoxic concentrations. Secondly, the extent of association of viral proteins and infectious virus particles with the cytoskeletal fraction of monkey Vero cells was determined by using three non-ionic detergents, Triton X-100 (TX-100), NP-40 and octyl glucoside (OG). The cytoskeleton retained nearly 70% of the external JV envelope glycoprotein GP38 and about 40% of the JV nucleoprotein NP, according to TX-100 and OG insolubility results. Furthermore, 1% of the total cell-bound infectivity was detected in the detergent-insoluble fraction, suggesting that cytoskeletal components are involved in the initiation of the assembly and budding processes of JV particles at the plasma membrane.
format	JOUR
author	Candurra, N.A. Lago, M.J. Maskin, L. Damonte, E.B.
author_facet	Candurra, N.A. Lago, M.J. Maskin, L. Damonte, E.B.
author_sort	Candurra, N.A.
title	Involvement of the cytoskeleton in Junin virus multiplication
title_short	Involvement of the cytoskeleton in Junin virus multiplication
title_full	Involvement of the cytoskeleton in Junin virus multiplication
title_fullStr	Involvement of the cytoskeleton in Junin virus multiplication
title_full_unstemmed	Involvement of the cytoskeleton in Junin virus multiplication
title_sort	involvement of the cytoskeleton in junin virus multiplication
url	http://hdl.handle.net/20.500.12110/paper_00221317_v80_n1_p147_Candurra
work_keys_str_mv	AT candurrana involvementofthecytoskeletoninjuninvirusmultiplication AT lagomj involvementofthecytoskeletoninjuninvirusmultiplication AT maskinl involvementofthecytoskeletoninjuninvirusmultiplication AT damonteeb involvementofthecytoskeletoninjuninvirusmultiplication
_version_	1807316611154051072

Involvement of the cytoskeleton in Junin virus multiplication

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