Persistent infection of Vero cells with Tacaribe virus

Persistently infected cultures have been established from Vero cells surviving primary infection with Tacaribe virus (Vero-T). The growth rate and morphological characteristics of the persistently infected cells were indistinguishable from normal Vero cells. Virus release declined during the first 6...

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Autores principales: Damonte, E.B., D'Aiutola, C., Coto, C.E.
Formato: JOUR
Materias:
animal experiment
cell culture
in vitro study
persistent infection
tacaribe arenavirus
Animalia
Arenavirus
Tacaribe virus
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00221317_v56_n1_p41_Damonte
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00221317_v56_n1_p41_Damonte
record_format dspace
spelling todo:paper_00221317_v56_n1_p41_Damonte2023-10-03T14:27:22Z Persistent infection of Vero cells with Tacaribe virus Damonte, E.B. D'Aiutola, C. Coto, C.E. animal experiment cell culture in vitro study persistent infection tacaribe arenavirus Animalia Arenavirus Tacaribe virus Persistently infected cultures have been established from Vero cells surviving primary infection with Tacaribe virus (Vero-T). The growth rate and morphological characteristics of the persistently infected cells were indistinguishable from normal Vero cells. Virus release declined during the first 6 passages, a cyclical pattern was observed between passages 6 and 16, and subsequently no virus infectivity could be detected. Co-cultivation with normal RK-13 or Vero cells enhanced virus yield from virus-producing cultures of Veri-T cells (passage 15), but the addition of susceptible cells had no effect on non-producer Vero-T cultures (passage 19). Only a small proportion (<1%) of the persistently infected cells tested during the first 16 passages produced infectious virus. The virus released during the early stages of persistence was temperature-sensitive if grown at 40°C, more thermolabile at 50°C than parental virus, and unable to initiate a persistent infection in Vero cells. Vero-T cells consistently showed refractoriness to homotypic Tacaribe virus superinfection and a selective graded resistance to other arenavirus replication. The possible use of viral susceptibility of persistently infected cultures as marker of antigenic relationship among Tacaribe complex viruses is considered. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221317_v56_n1_p41_Damonte
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic animal experiment
cell culture
in vitro study
persistent infection
tacaribe arenavirus
Animalia
Arenavirus
Tacaribe virus
spellingShingle animal experiment
cell culture
in vitro study
persistent infection
tacaribe arenavirus
Animalia
Arenavirus
Tacaribe virus
Damonte, E.B.
D'Aiutola, C.
Coto, C.E.
Persistent infection of Vero cells with Tacaribe virus
topic_facet animal experiment
cell culture
in vitro study
persistent infection
tacaribe arenavirus
Animalia
Arenavirus
Tacaribe virus
description Persistently infected cultures have been established from Vero cells surviving primary infection with Tacaribe virus (Vero-T). The growth rate and morphological characteristics of the persistently infected cells were indistinguishable from normal Vero cells. Virus release declined during the first 6 passages, a cyclical pattern was observed between passages 6 and 16, and subsequently no virus infectivity could be detected. Co-cultivation with normal RK-13 or Vero cells enhanced virus yield from virus-producing cultures of Veri-T cells (passage 15), but the addition of susceptible cells had no effect on non-producer Vero-T cultures (passage 19). Only a small proportion (<1%) of the persistently infected cells tested during the first 16 passages produced infectious virus. The virus released during the early stages of persistence was temperature-sensitive if grown at 40°C, more thermolabile at 50°C than parental virus, and unable to initiate a persistent infection in Vero cells. Vero-T cells consistently showed refractoriness to homotypic Tacaribe virus superinfection and a selective graded resistance to other arenavirus replication. The possible use of viral susceptibility of persistently infected cultures as marker of antigenic relationship among Tacaribe complex viruses is considered.
format JOUR
author Damonte, E.B.
D'Aiutola, C.
Coto, C.E.
author_facet Damonte, E.B.
D'Aiutola, C.
Coto, C.E.
author_sort Damonte, E.B.
title Persistent infection of Vero cells with Tacaribe virus
title_short Persistent infection of Vero cells with Tacaribe virus
title_full Persistent infection of Vero cells with Tacaribe virus
title_fullStr Persistent infection of Vero cells with Tacaribe virus
title_full_unstemmed Persistent infection of Vero cells with Tacaribe virus
title_sort persistent infection of vero cells with tacaribe virus
url http://hdl.handle.net/20.500.12110/paper_00221317_v56_n1_p41_Damonte
work_keys_str_mv AT damonteeb persistentinfectionofverocellswithtacaribevirus
AT daiutolac persistentinfectionofverocellswithtacaribevirus
AT cotoce persistentinfectionofverocellswithtacaribevirus
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