Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens

The incubation of an enzyme preparation of Agrobacterium tumefaciens with UDP-[ 14 C]Glc led to the formation of radioactive substances soluble in chloroform/methanol/water (1:1:0.3, by vol.). These substances had properties similar to the polyprenol diphosphate saccharides. The lipid moiety appears...

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Autores principales:	Staneloni, R.J., Tolmasky, M.E., Leloir, L.F.
Formato:	JOUR
Materias:	lipid lipopolysaccharide polysaccharide radioisotope unclassified drug uridine diphosphate glucose c 14 nonhuman Rhizobium radiobacter Agrobacterium tumefaciens
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00221287_v130_n4_p869_Staneloni
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00221287_v130_n4_p869_Staneloni
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spelling	todo:paper_00221287_v130_n4_p869_Staneloni2023-10-03T14:27:20Z Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens Staneloni, R.J. Tolmasky, M.E. Leloir, L.F. lipid lipopolysaccharide polysaccharide radioisotope unclassified drug uridine diphosphate glucose c 14 nonhuman Rhizobium radiobacter Agrobacterium tumefaciens The incubation of an enzyme preparation of Agrobacterium tumefaciens with UDP-[ 14 C]Glc led to the formation of radioactive substances soluble in chloroform/methanol/water (1:1:0.3, by vol.). These substances had properties similar to the polyprenol diphosphate saccharides. The lipid moiety appears to be an unsaturated polyprenol. Mild acid hydrolysis of the substances liberated five water-soluble products which were separated by TLC and characterized by borohydride reduction-acid hydrolysis, partial acid hydrolysis, methylation analysis, TLC and paper chromatography and paper electrophoresis. The products behaved like: galactose; the disaccharide Glc(β1-3)Gal; the tetrasaccharide Glc(β1-4)Glc(β1-4)Glc(β1-3)Gal; an octasaccharide and a pyruvylated octasaccharide. The last two substances were compared by paper electrophoresis, TLC and methylation analysis with an octasaccharide obtained by the action of a hydrolytic enzyme on the exopolysaccharide of Agrobacterium tumefaciens. The octasaccharide and the pyruvylated octasaccharide from the lipid behaved like those obtained by enzyme action on the A. tumefaciens exopolysaccharide. These results suggest that the lipid-bound saccharides are involved in the biosynthesis of the extracellular polysaccharides. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00221287_v130_n4_p869_Staneloni
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	lipid lipopolysaccharide polysaccharide radioisotope unclassified drug uridine diphosphate glucose c 14 nonhuman Rhizobium radiobacter Agrobacterium tumefaciens
spellingShingle	lipid lipopolysaccharide polysaccharide radioisotope unclassified drug uridine diphosphate glucose c 14 nonhuman Rhizobium radiobacter Agrobacterium tumefaciens Staneloni, R.J. Tolmasky, M.E. Leloir, L.F. Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens
topic_facet	lipid lipopolysaccharide polysaccharide radioisotope unclassified drug uridine diphosphate glucose c 14 nonhuman Rhizobium radiobacter Agrobacterium tumefaciens
description	The incubation of an enzyme preparation of Agrobacterium tumefaciens with UDP-[ 14 C]Glc led to the formation of radioactive substances soluble in chloroform/methanol/water (1:1:0.3, by vol.). These substances had properties similar to the polyprenol diphosphate saccharides. The lipid moiety appears to be an unsaturated polyprenol. Mild acid hydrolysis of the substances liberated five water-soluble products which were separated by TLC and characterized by borohydride reduction-acid hydrolysis, partial acid hydrolysis, methylation analysis, TLC and paper chromatography and paper electrophoresis. The products behaved like: galactose; the disaccharide Glc(β1-3)Gal; the tetrasaccharide Glc(β1-4)Glc(β1-4)Glc(β1-3)Gal; an octasaccharide and a pyruvylated octasaccharide. The last two substances were compared by paper electrophoresis, TLC and methylation analysis with an octasaccharide obtained by the action of a hydrolytic enzyme on the exopolysaccharide of Agrobacterium tumefaciens. The octasaccharide and the pyruvylated octasaccharide from the lipid behaved like those obtained by enzyme action on the A. tumefaciens exopolysaccharide. These results suggest that the lipid-bound saccharides are involved in the biosynthesis of the extracellular polysaccharides.
format	JOUR
author	Staneloni, R.J. Tolmasky, M.E. Leloir, L.F.
author_facet	Staneloni, R.J. Tolmasky, M.E. Leloir, L.F.
author_sort	Staneloni, R.J.
title	Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens
title_short	Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens
title_full	Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens
title_fullStr	Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens
title_full_unstemmed	Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens
title_sort	lipid-bound saccharides containing glucose and galactose in agrobacterium tumefaciens
url	http://hdl.handle.net/20.500.12110/paper_00221287_v130_n4_p869_Staneloni
work_keys_str_mv	AT stanelonirj lipidboundsaccharidescontainingglucoseandgalactoseinagrobacteriumtumefaciens AT tolmaskyme lipidboundsaccharidescontainingglucoseandgalactoseinagrobacteriumtumefaciens AT leloirlf lipidboundsaccharidescontainingglucoseandgalactoseinagrobacteriumtumefaciens
_version_	1807314583010934784

Lipid-bound saccharides containing glucose and galactose in Agrobacterium tumefaciens

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