Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells

The actions of several neuropeptides as hypothalamic mediators in the regulation of Bufo arenarum metamorphosis were investigated. Prometamorphic larvae were injected with 1.5 μg thyrotropin-releasing hormone (TRH), 2 μg ovine corticotropin-releasing factor (oCRF), 2 μg mammalian gonadotropin-releas...

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Autores principales: Miranda, L.A., Affanni, J.M., Paz, D.A.
Formato: JOUR
Materias:
Animalia
Anura
Bufo arenarum
Mammalia
Ovis
Vertebrata
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0022104X_v286_n5_p473_Miranda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_0022104X_v286_n5_p473_Miranda2023-10-03T14:26:25Z Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells Miranda, L.A. Affanni, J.M. Paz, D.A. Animalia Anura Bufo arenarum Bufo arenarum Mammalia Ovis Vertebrata The actions of several neuropeptides as hypothalamic mediators in the regulation of Bufo arenarum metamorphosis were investigated. Prometamorphic larvae were injected with 1.5 μg thyrotropin-releasing hormone (TRH), 2 μg ovine corticotropin-releasing factor (oCRF), 2 μg mammalian gonadotropin-releasing hormone (mGnRH), 2 μg human growth hormone-releasing hormone (hGHRH), or Holtfreter solution (control group). Larvae received two injections with the same dose: one at the beginning of the experiment and the other 7 days later. Several morphologic parameters (total length, tail length, wet weight, hind limb length, and metamorphic stages) were measured as indicators of growth and metamorphic development. These measurements were taken in 20 larvae per treatment or control group at the beginning of the experiment, at day 7 and at day 14 when the experiment ended. We observed that only the administration of exogenous CRF stimulated resorption of the tail and accelerated the rate of metamorphosis. In the pituitary of CRF-treated larvae we observed that thyrotropin (TSH) and adrenocorticotropic hormone (ACTH) producing cells showed a weaker immunoreactivity, a decrease in cell number and a reduction of volume density when compared with normal larvae. In conclusion, the results obtained indicate a possible role for CRF in Bufo arenarum metamorphosis. CRF may regulate interrenal and thyroid activity by acting directly upon TSH and ACTH cells. On the other hand, TRH, GnRH and GHRH were inactive in stimulating growth or metamorphosis of Bufo arenarum. (C) 2000 Wiley-Liss, Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0022104X_v286_n5_p473_Miranda
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Animalia
Anura
Bufo arenarum
Bufo arenarum
Mammalia
Ovis
Vertebrata
spellingShingle Animalia
Anura
Bufo arenarum
Bufo arenarum
Mammalia
Ovis
Vertebrata
Miranda, L.A.
Affanni, J.M.
Paz, D.A.
Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells
topic_facet Animalia
Anura
Bufo arenarum
Bufo arenarum
Mammalia
Ovis
Vertebrata
description The actions of several neuropeptides as hypothalamic mediators in the regulation of Bufo arenarum metamorphosis were investigated. Prometamorphic larvae were injected with 1.5 μg thyrotropin-releasing hormone (TRH), 2 μg ovine corticotropin-releasing factor (oCRF), 2 μg mammalian gonadotropin-releasing hormone (mGnRH), 2 μg human growth hormone-releasing hormone (hGHRH), or Holtfreter solution (control group). Larvae received two injections with the same dose: one at the beginning of the experiment and the other 7 days later. Several morphologic parameters (total length, tail length, wet weight, hind limb length, and metamorphic stages) were measured as indicators of growth and metamorphic development. These measurements were taken in 20 larvae per treatment or control group at the beginning of the experiment, at day 7 and at day 14 when the experiment ended. We observed that only the administration of exogenous CRF stimulated resorption of the tail and accelerated the rate of metamorphosis. In the pituitary of CRF-treated larvae we observed that thyrotropin (TSH) and adrenocorticotropic hormone (ACTH) producing cells showed a weaker immunoreactivity, a decrease in cell number and a reduction of volume density when compared with normal larvae. In conclusion, the results obtained indicate a possible role for CRF in Bufo arenarum metamorphosis. CRF may regulate interrenal and thyroid activity by acting directly upon TSH and ACTH cells. On the other hand, TRH, GnRH and GHRH were inactive in stimulating growth or metamorphosis of Bufo arenarum. (C) 2000 Wiley-Liss, Inc.
format JOUR
author Miranda, L.A.
Affanni, J.M.
Paz, D.A.
author_facet Miranda, L.A.
Affanni, J.M.
Paz, D.A.
author_sort Miranda, L.A.
title Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells
title_short Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells
title_full Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells
title_fullStr Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells
title_full_unstemmed Corticotropin-releasing factor accelerates metamorphosis in Bufo arenarum: Effect on pituitary ACTH and TSH cells
title_sort corticotropin-releasing factor accelerates metamorphosis in bufo arenarum: effect on pituitary acth and tsh cells
url http://hdl.handle.net/20.500.12110/paper_0022104X_v286_n5_p473_Miranda
work_keys_str_mv AT mirandala corticotropinreleasingfactoracceleratesmetamorphosisinbufoarenarumeffectonpituitaryacthandtshcells
AT affannijm corticotropinreleasingfactoracceleratesmetamorphosisinbufoarenarumeffectonpituitaryacthandtshcells
AT pazda corticotropinreleasingfactoracceleratesmetamorphosisinbufoarenarumeffectonpituitaryacthandtshcells
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