The absence of DNA from the blood serum of Bufo arenarum

The absence of DNA from the blood serum of the toad Bufo arenarum was confirmed by classical colorimetric techniques and by a new method which allows differentiation between DNA and the glycosaminoglycans (GAG). A component of serum which had some of the properties of DNA was identified as a GAG. Th...

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Detalles Bibliográficos
Autores principales: De Recondo, M.E.F., De Arcuri, B.E.F., Recondo, E.F.
Formato: JOUR
Materias:
DNA
glycosaminoglycan
animal
article
blood
colorimetry
isolation and purification
methodology
spectrophotometry
toad
Animal
Bufo arenarum
Colorimetry
Glycosaminoglycans
Spectrophotometry
Support, Non-U.S. Gov't
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0022104X_v243_n3_p445_DeRecondo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The absence of DNA from the blood serum of the toad Bufo arenarum was confirmed by classical colorimetric techniques and by a new method which allows differentiation between DNA and the glycosaminoglycans (GAG). A component of serum which had some of the properties of DNA was identified as a GAG. This component gave positive reactions in colorimetric assays for DNA, but the absorption spectra were completely different from those of authentic DNA. The compound was also not hydrolized at all by treatment with deoxyribonuclease. Copyright © 1987 Wiley‐Liss, Inc., A Wiley Company

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