Fluctuation of oxidative stress indicators in Salix nigra seeds during priming
Salix nigra seeds subjected to increased humidification show a decrease in normal germination (NG) during early imbibition followed by a recovery in that parameter at increasing imbibition times. Since photo-oxidized seeds contain high levels of reactive oxygen species (ROS), it is possible to infer...
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todo:paper_00220957_v63_n10_p3631_Roqueiro2023-10-03T14:26:15Z Fluctuation of oxidative stress indicators in Salix nigra seeds during priming Roqueiro, G. Maldonado, S. Ríos, M.D.C. Maroder, H. Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra Salix nigra seeds subjected to increased humidification show a decrease in normal germination (NG) during early imbibition followed by a recovery in that parameter at increasing imbibition times. Since photo-oxidized seeds contain high levels of reactive oxygen species (ROS), it is possible to infer that the atypical decrease in NG is a consequence of a higher ROS mobilization at early imbibition and the subsequent recovery from an increase in antioxidant activity. In this study, several oxidative stress indicators were evaluated in photo-oxidized seeds subjected to priming. ROS production was studied using electronic spin resonance spectroscopy, spontaneous chemiluminescence (SCL), spectrophotometry (with XTT), and histochemical (with DAB and NBT) and cytochemical (with CeCl3) techniques. Four indicators of molecular damage were monitored: lipid peroxidation, pigment destruction, protein oxidation, and membrane integrity. Antioxidant activity was evaluated by changes in the enzymes SOD, CAT, APX, and POX. The results revealed that the decrease in NG at the beginning of priming occurs by an oxidative burst, as determined by increases in both SCL and superoxide anion radical (O2·-). Such oxidative burst generates lipid peroxidation, protein oxidation, and a decrease in both pigment content and enzyme activities. With increasing hydration, damages are progressively reversed and NG restored, which coincides with the increased activity of antioxidant defences. It is proposed that these novel observations regarding the occurrence of an oxidative burst are related to the high basal ROS levels and the high membrane content retained in the mature embryo tissues. © 2012 The Author. Fil:Roqueiro, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Maroder, H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220957_v63_n10_p3631_Roqueiro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra |
| spellingShingle |
Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra Roqueiro, G. Maldonado, S. Ríos, M.D.C. Maroder, H. Fluctuation of oxidative stress indicators in Salix nigra seeds during priming |
| topic_facet |
Antioxidant enzymes lipid peroxidation normal germination oxidative burst priming reactive oxygen species (ROS) reactive oxygen metabolite vegetable protein water article germination growth, development and aging metabolism oxidative stress plant seed willow Germination Oxidative Stress Plant Proteins Reactive Oxygen Species Salix Seeds Water Salix nigra |
| description |
Salix nigra seeds subjected to increased humidification show a decrease in normal germination (NG) during early imbibition followed by a recovery in that parameter at increasing imbibition times. Since photo-oxidized seeds contain high levels of reactive oxygen species (ROS), it is possible to infer that the atypical decrease in NG is a consequence of a higher ROS mobilization at early imbibition and the subsequent recovery from an increase in antioxidant activity. In this study, several oxidative stress indicators were evaluated in photo-oxidized seeds subjected to priming. ROS production was studied using electronic spin resonance spectroscopy, spontaneous chemiluminescence (SCL), spectrophotometry (with XTT), and histochemical (with DAB and NBT) and cytochemical (with CeCl3) techniques. Four indicators of molecular damage were monitored: lipid peroxidation, pigment destruction, protein oxidation, and membrane integrity. Antioxidant activity was evaluated by changes in the enzymes SOD, CAT, APX, and POX. The results revealed that the decrease in NG at the beginning of priming occurs by an oxidative burst, as determined by increases in both SCL and superoxide anion radical (O2·-). Such oxidative burst generates lipid peroxidation, protein oxidation, and a decrease in both pigment content and enzyme activities. With increasing hydration, damages are progressively reversed and NG restored, which coincides with the increased activity of antioxidant defences. It is proposed that these novel observations regarding the occurrence of an oxidative burst are related to the high basal ROS levels and the high membrane content retained in the mature embryo tissues. © 2012 The Author. |
| format |
JOUR |
| author |
Roqueiro, G. Maldonado, S. Ríos, M.D.C. Maroder, H. |
| author_facet |
Roqueiro, G. Maldonado, S. Ríos, M.D.C. Maroder, H. |
| author_sort |
Roqueiro, G. |
| title |
Fluctuation of oxidative stress indicators in Salix nigra seeds during priming |
| title_short |
Fluctuation of oxidative stress indicators in Salix nigra seeds during priming |
| title_full |
Fluctuation of oxidative stress indicators in Salix nigra seeds during priming |
| title_fullStr |
Fluctuation of oxidative stress indicators in Salix nigra seeds during priming |
| title_full_unstemmed |
Fluctuation of oxidative stress indicators in Salix nigra seeds during priming |
| title_sort |
fluctuation of oxidative stress indicators in salix nigra seeds during priming |
| url |
http://hdl.handle.net/20.500.12110/paper_00220957_v63_n10_p3631_Roqueiro |
| work_keys_str_mv |
AT roqueirog fluctuationofoxidativestressindicatorsinsalixnigraseedsduringpriming AT maldonados fluctuationofoxidativestressindicatorsinsalixnigraseedsduringpriming AT riosmdc fluctuationofoxidativestressindicatorsinsalixnigraseedsduringpriming AT maroderh fluctuationofoxidativestressindicatorsinsalixnigraseedsduringpriming |
| _version_ |
1782028276244414464 |