Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress
The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activit...
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todo:paper_00220957_v60_n15_p4249_Rodriguez2023-10-03T14:26:14Z Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress Rodríguez, A.A. Maiale, S.J. Menéndez, A.B. Ruiz, O.A. Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays Zea mays The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activity of polyamine oxidase was up to 20-fold higher than that of the copper-containing amine oxidase. Measurements of H2O2, ·O2-, and HO· production in the presence or absence of the polyamine oxidase inhibitors 1,19-bis- (ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane suggest that, in salinized plants, the oxidation of free apoplastic polyamines by polyamine oxidase by would be the main source of reactive oxygen species in the elongation zone of maize leaf blades. This effect is probably due to increased substrate availability. Incubation with 200 μM spermine doubled segment elongation, whereas the addition of 1,19-bis-(ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane to 200 μM spermine attenuated and reversed the last effect, respectively. Similarly, the addition of MnCl2 (an ·O2- dismutating agent) or the HO· scavenger sodium benzoate along with spermine, annulled the elongating effect of the polyamine on the salinized segments. As a whole, the results obtained here demonstrated that, under salinity, polyamine oxidase activity provides a significant production of reactive oxygen species in the apoplast which contributes to 25-30% of the maize leaf blade elongation. Fil:Rodríguez, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Menéndez, A.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220957_v60_n15_p4249_Rodriguez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays Zea mays |
| spellingShingle |
Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays Zea mays Rodríguez, A.A. Maiale, S.J. Menéndez, A.B. Ruiz, O.A. Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress |
| topic_facet |
Growth Maize Polyamine oxidase Polyamines Reactive oxygen species Salinity oxidoreductase polyamine oxidase sodium chloride vegetable protein article enzymology growth, development and aging maize metabolism physiological stress physiology plant leaf Oxidoreductases Acting on CH-NH Group Donors Plant Leaves Plant Proteins Sodium Chloride Stress, Physiological Zea mays Zea mays |
| description |
The possible involvement of apoplastic reactive oxygen species produced by the oxidation of free polyamines in the leaf growth of salinized maize has been studied here. Salt treatment increased the apoplastic spermine and spermidine levels, mainly in the leaf blade elongation zone. The total activity of polyamine oxidase was up to 20-fold higher than that of the copper-containing amine oxidase. Measurements of H2O2, ·O2-, and HO· production in the presence or absence of the polyamine oxidase inhibitors 1,19-bis- (ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane suggest that, in salinized plants, the oxidation of free apoplastic polyamines by polyamine oxidase by would be the main source of reactive oxygen species in the elongation zone of maize leaf blades. This effect is probably due to increased substrate availability. Incubation with 200 μM spermine doubled segment elongation, whereas the addition of 1,19-bis-(ethylamine)-5,10,15 triazanonadecane and 1,8-diamino-octane to 200 μM spermine attenuated and reversed the last effect, respectively. Similarly, the addition of MnCl2 (an ·O2- dismutating agent) or the HO· scavenger sodium benzoate along with spermine, annulled the elongating effect of the polyamine on the salinized segments. As a whole, the results obtained here demonstrated that, under salinity, polyamine oxidase activity provides a significant production of reactive oxygen species in the apoplast which contributes to 25-30% of the maize leaf blade elongation. |
| format |
JOUR |
| author |
Rodríguez, A.A. Maiale, S.J. Menéndez, A.B. Ruiz, O.A. |
| author_facet |
Rodríguez, A.A. Maiale, S.J. Menéndez, A.B. Ruiz, O.A. |
| author_sort |
Rodríguez, A.A. |
| title |
Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress |
| title_short |
Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress |
| title_full |
Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress |
| title_fullStr |
Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress |
| title_full_unstemmed |
Polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress |
| title_sort |
polyamine oxidase activity contributes to sustain maize leaf elongation under saline stress |
| url |
http://hdl.handle.net/20.500.12110/paper_00220957_v60_n15_p4249_Rodriguez |
| work_keys_str_mv |
AT rodriguezaa polyamineoxidaseactivitycontributestosustainmaizeleafelongationundersalinestress AT maialesj polyamineoxidaseactivitycontributestosustainmaizeleafelongationundersalinestress AT menendezab polyamineoxidaseactivitycontributestosustainmaizeleafelongationundersalinestress AT ruizoa polyamineoxidaseactivitycontributestosustainmaizeleafelongationundersalinestress |
| _version_ |
1807323401012903936 |