StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration
StCDPK1 encodes a calcium-dependent protein kinase (CDPK) from Solanum tuberosum, which is transiently induced upon tuberization in swelling stolons. In situ hybridization determined that StCDPK1 mRNA is localized in the apical dome of tuberizing stolon tips, close to the region where sucrose was re...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00220957_v54_n392_p2589_Raices |
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todo:paper_00220957_v54_n392_p2589_Raices2023-10-03T14:26:12Z StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration Raíces, M. Ulloa, R.M. MacIntosh, G.C. Crespi, M. Téllez-Iñón, M.T. Gene expression Okadaic acid Potato stolons StCDPK1 Sucrose Enzymes Organic acids Plants (botany) RNA Sugar (sucrose) Swelling Hybridization Genetic engineering calcium binding protein CDPK1 protein, plant okadaic acid protein kinase sucrose vegetable protein article drug effect enzymology gene expression regulation genetics kinetics photoperiodicity potato Calcium-Binding Proteins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Kinetics Okadaic Acid Photoperiod Plant Proteins Protein Kinases Solanum tuberosum Sucrose Embryophyta Eukaryota Solanum Solanum tuberosum StCDPK1 encodes a calcium-dependent protein kinase (CDPK) from Solanum tuberosum, which is transiently induced upon tuberization in swelling stolons. In situ hybridization determined that StCDPK1 mRNA is localized in the apical dome of tuberizing stolon tips, close to the region where sucrose was reported to accumulate. The expression of StCDPK1, and other tuber-specific genes was enhanced when in vitro-cultured potato plants were transferred to high sucrose or high sorbitol containing media. Glucose, fructose or a mixture of both showed no effect on CDPK expression. Okadaic acid blocked sucrose-inducible gene expression, suggesting that phosphatases from the PP1/PP2A family could also participate in the regulation of StCDPK1 and other tuberization-related genes. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220957_v54_n392_p2589_Raices |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Gene expression Okadaic acid Potato stolons StCDPK1 Sucrose Enzymes Organic acids Plants (botany) RNA Sugar (sucrose) Swelling Hybridization Genetic engineering calcium binding protein CDPK1 protein, plant okadaic acid protein kinase sucrose vegetable protein article drug effect enzymology gene expression regulation genetics kinetics photoperiodicity potato Calcium-Binding Proteins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Kinetics Okadaic Acid Photoperiod Plant Proteins Protein Kinases Solanum tuberosum Sucrose Embryophyta Eukaryota Solanum Solanum tuberosum |
| spellingShingle |
Gene expression Okadaic acid Potato stolons StCDPK1 Sucrose Enzymes Organic acids Plants (botany) RNA Sugar (sucrose) Swelling Hybridization Genetic engineering calcium binding protein CDPK1 protein, plant okadaic acid protein kinase sucrose vegetable protein article drug effect enzymology gene expression regulation genetics kinetics photoperiodicity potato Calcium-Binding Proteins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Kinetics Okadaic Acid Photoperiod Plant Proteins Protein Kinases Solanum tuberosum Sucrose Embryophyta Eukaryota Solanum Solanum tuberosum Raíces, M. Ulloa, R.M. MacIntosh, G.C. Crespi, M. Téllez-Iñón, M.T. StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration |
| topic_facet |
Gene expression Okadaic acid Potato stolons StCDPK1 Sucrose Enzymes Organic acids Plants (botany) RNA Sugar (sucrose) Swelling Hybridization Genetic engineering calcium binding protein CDPK1 protein, plant okadaic acid protein kinase sucrose vegetable protein article drug effect enzymology gene expression regulation genetics kinetics photoperiodicity potato Calcium-Binding Proteins Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Kinetics Okadaic Acid Photoperiod Plant Proteins Protein Kinases Solanum tuberosum Sucrose Embryophyta Eukaryota Solanum Solanum tuberosum |
| description |
StCDPK1 encodes a calcium-dependent protein kinase (CDPK) from Solanum tuberosum, which is transiently induced upon tuberization in swelling stolons. In situ hybridization determined that StCDPK1 mRNA is localized in the apical dome of tuberizing stolon tips, close to the region where sucrose was reported to accumulate. The expression of StCDPK1, and other tuber-specific genes was enhanced when in vitro-cultured potato plants were transferred to high sucrose or high sorbitol containing media. Glucose, fructose or a mixture of both showed no effect on CDPK expression. Okadaic acid blocked sucrose-inducible gene expression, suggesting that phosphatases from the PP1/PP2A family could also participate in the regulation of StCDPK1 and other tuberization-related genes. |
| format |
JOUR |
| author |
Raíces, M. Ulloa, R.M. MacIntosh, G.C. Crespi, M. Téllez-Iñón, M.T. |
| author_facet |
Raíces, M. Ulloa, R.M. MacIntosh, G.C. Crespi, M. Téllez-Iñón, M.T. |
| author_sort |
Raíces, M. |
| title |
StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration |
| title_short |
StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration |
| title_full |
StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration |
| title_fullStr |
StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration |
| title_full_unstemmed |
StCDPK1 is expressed in potato stolon tips and is induced by high sucrose concentration |
| title_sort |
stcdpk1 is expressed in potato stolon tips and is induced by high sucrose concentration |
| url |
http://hdl.handle.net/20.500.12110/paper_00220957_v54_n392_p2589_Raices |
| work_keys_str_mv |
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